Interaction between Eichornia crassipes with Microbacterium sp. SpR3 and Micrococcus luteus RT-9 in Cr6+ reduction in liquid media




Abstract. Wulandari AD, Meitiniarti VI, Kasmiyati S. 2024. Interaction between Eichornia crassipes with Microbacterium sp. SpR3 and Micrococcus luteus RT-9 in Cr6+ reduction in liquid media. Biodiversitas 25: 957-963. Water Pollution caused by Cr6+ contamination is difficult to remove from the environment. One of the efforts to reduce Cr6+ contamination in water is by bioremediation. Bioremediation using the interaction of rhizosphere bacteria with aquatic plants can be employed to decrease the content of Cr6+ in water environment. This study aimed to determine the effect of adding Microbacterium sp. SpR3, Micrococcus luteus RT-9, and a mixture of both bacteria on the ability of Eichornia crassipes to reduce the concentration of Cr6+ in the growth media and the plant's growth potential. This study was conducted in the Microbiology Laboratory of the Faculty of Biology at Satya Wacana Christian University. Eichornia crassipes plans of similar size were grown in 450 mL of Hoaglands solution with Cr6+ 5 mg.L-1 in a container. The treatment in this study involves the addition of 50 mL (10% of the working volume) of Microbacterium sp SpR3 culture, Micrococcus luteus RT- 9, and a combination of both Cr6+-reducing bacteria. The reduction rate of Cr6+ concentration in the mixture of both types of bacteria reached 88.06%, which was higher compared to the control without the addition of the inoculum (87.08%). The inoculation of both Cr6+ reducing bacteria did not hibit the growth rate of the plants. The ability of Microbacterium sp. SpR3 and Micrococcus luteus RT-9 to reduce Cr6+ in liquid media can be employed in the bioremediation of Cr6+ contaminated wastewater.


Abou-shanab, R. A. I., J. S. Angel, dan P. V. Berkum. 2007. Chromate-Tolerant Bacteria For Enhanced Metal Uptake By Eichhornia crassipes (Mart.). International Journal Of Phytoremediation 9(1): 91-105.
Agarwal, A., H. P. Singh, dan J. P. N. Rai. 2013. Bioremediation of tannery effluent using Pseudomonas fluorescens and Eichhornia crassipes and its effect on Wheat seed germination and plant growth. G-Journal of Environmental Science and Technology 1(1): 20–23.
Ahemad, M. 2015. Enhancing phytoremediation of chromium-stressed soils through plant-growth-promoting bacteria. Journal of Genetic Engineering and Biotechnology 13(1): 51-58.
Andini, A. 2017. Analysis of Chromium VI Content in Water in Tanggulangin Distric, Sidoarjo. Journal Science Health 1(2): 2–5
Arslan, M., A. Imran, dan Q. M. Khan. 2017. Plant–bacteria partnerships for the remediation of persistent organic pollutants. Environment Science Pollution Research 24(1): 4322-4336.
Ashraf, S., Afzal, M., Naveed, M. 2018. Endophytic bacteria enhance the remediation of tannery effluent in constructed wetlands vegetated with Leptochloa fusca. International Journal of Phytoremediation 20(2): 121-128.
Augustynowicz, J., ?. Krzysztof, K. Tokarz, dan B. Jan. 2015. Potential for chromium (VI) bioremediation by the aquatic carnivorous plant Utricularia gibba L . (Lentibulariaceae). Environment Science Pollution Research 22(1): 9742–9748
Coelho, L. M., H. C. Rezende, P. A. R. De Sousa, D. F. O. Melo, dan N. M. M. Coelho. 2015. Bioremediation of Polluted Waters Using Microorganisms. INTECH 1(1): 1–22.
Costa, A. C. A., and F. P. Duta. 2001. Bioaccumulation of copper, zinc, cadmium, and lead by Bacillus sp., Bacillus cereus, Bacillus sphaericus and Bacillus subtilis. Brazillian Journal of Microbiology 2001 (32): 1-5.
Faisal, M., dan S. Hasnain. 2003. Synergistic Removal of Cr(VI) by Eichornia crassipes in Conjuction With Bacterial Strains. Pakistan Journal of Bioremediation Science 6(3): 264-268
Handayani, R. I. 2015. The accumulation of Heavy Metal Chromium (Cr) in the Meat of Red Tilapia (Oreochromis sp) in Floating Net Cages in The Wonogiri River. Yogyakarta. Skripsi: UNNES.
Herdina, S. P., Budiyono, dan Suhartono. 2018. Effectiveness of Old Variation and Contact of Water Hyacinth (Eichornia crassipes) in Reducing Heavy Metal Hexavalent Chromium (Cr6+) in Metallized Coating Industry Waste. Jurnal Kesehatan Masyarakat 6(6): 315-324.
Innation, T.O., V. I. Meitiniarti, dan D. C. Cahyaningrum. 2021. The Reduction Of Cr(VI) in Soil By Microbacterium sp. Strain SpR3 in Vermicompost Carrier. Jurnal Bioteknologi Biosains Indonesia 8(1): 33–41.
Irawati, W., A. Jan, N. Parhusip, N. Sopiah, dan J. A. Tnunay. 2017. The Role of Heavy Metals-Resistant Bacteria Acinetobacter sp . in Copper Phytoremediation using Eichhornia crassipes Mart. Solms. International Conference on Natural Research and Life Science.
Laet, C. D., T. Matringe, E. Petit, dan C. Grison. 2019. Eichhornia crassipes?: a Powerful Bio-indicator for Water Pollution by Emerging Pollutants. Scientific Reports 9(7326): 1-10.
Meitiniarti, V. I., Nugroho R. A., Krave A. S. 2014. Variety of chromium-reducing bacteria from tannery wastewater and rhizosphere of Acalypha indica. Prosiding Seminar Nasional Mikrobiologi: Keanekaragaman dan Pemanfaatan Sumber daya Mikroba Tropika Indonesia. Salatiga: 4 Agustus 2014: 59-63.
Meitiniarti, V. I., E. K. Putri, A. E. Runtu, R. A. Nugroho, dan S. Kasmiyati. 2022. Isolation and identification of Cr-resistant bacteria from the rhizosphere of Tagetes sp. And their ability to reduce Cr(VI). Biodiversitas 23(8): 4117-4123.
Nazir, M. I., I. Idrees, P. Danish, S. Ahmad, Q. Ali, A. Malik. 2020. The potential of Water Hyacinth (Eichornia crassipes) for phytoremediation of Heavy Metal From Waste Water. Biological and Clinical Sciences Research Journal 6(1): 1-6.
Nugroho R. A, V. I. Meitiniarti, dan D. Batunan. 2015. Potential isolates of Microbacterium sp SpR3 and Mezorhizobium sp SpR17 in reducing Cr(VI) in soil. Proc. Annual Meeting Indonesian Society for Microbiology: The contribution of microbes in improving the quality of human life. Semarang, Indonesia, 8-9 October 2015 [Indonesian].
Nurmalasari, A., Oedjijono, dan S. Lestari. 2020. Isolation and In-Vitro Resistance test of Water Hyacinth (Eichornia crassipes) Endophytic Bacteria. Bioeksakta: Jurnal Ilmiah Biologi Unsoed 2(2): 266–272.
Obinna, I. B., dan E. C. Ebere 2019. Phytoremediation of Polluted Waterbodies with Aquatic Plants?: Recent Progress on Heavy Metal and Organic Pollutants. Analytical Methods in Environmental Chemistry Journal 2019: 1-38
Ojuederie, O. B., dan O. O. Babalola. 2017. Microbial and Plant-Assisted Bioremediation of Heavy Metal Polluted Environments?: A Review. International Journal of Environment Research and Public Health 2017 (14): 1-26.
Pramono, A., R. Rosariastuti, Ngadiman, dan D. Irfan. 2013. Bacterial Cr (VI) Reduction and Its Impact In Bioremediation. Jurnal Ilmu Lingkungan 11(2): 120–131
Priyanto, G. Y. 2006. Bioremediation of household waste with a water plant simulation system. Jurnal Bumi Lestari, 8:136- 144.
Riva, V., F. Mapeli, E. Syranidou, E. Crotti, R. Choukrallah, N. Kalogerakis, dan S. Borin. 2019. Root Bacteria Recruited by Phragmites Australis in Constructed Wetlands Have The Potential to Enhance Azo-Dye Phytodepuration. Microorganism 2019(7): 1-21.
Sari, A. M., F. Rachmadiarti, dan H. Fithridayati. 2014. Effect of Chromium Stress in Batik Watewater on the Growth of Eichornia crassipes and Salvinia molesta. LenteraBio 3(1): 67-71.
Setianingrum, I., E. D. Sintadani, V. Viani, D. Uuliyah, M. F. Faridani, dan R. S. Putra. 2017. Eration Method (Combined Process Electro-Assisted Phytoremediation and Aeration) with Vetiver Roots (Vetiveira zizanioides) for Remediation of Fe and Cu Metal Wastewater. Chimica et Natura Acta 5(3): 112–119.
Singh, G., dan A. Sinha. 2011. Phytoremediation of chromium(VI)-laden waste by Eichornia crassipes. International Journal Environmental Technology and Management 14(1): 33-45.
Suryatman, P., M. R. Setiawati, N. N. Kamaludin, dan D. Herdiyantoro. 2022. The Effectiveness of Acetobacter sp. And Pseudomonas sp. On Sorgum plants (Shorgum bicolor L) in The process of Phytoremediation of Petroleum Waste. Jurnal Argo 9(2): 294-307.
Tabinda, A. B., R. Irfan, A. Yasar, A. Iqbal, dan A, Mahmood. 2018. Phytoremediation potential of Pistia stratiotes and Eichhornia crassipes to remove chromium and copper. Environment Technology 2018(1): 1-6.
Vidyawati, D. S., dan H. Fitrihidjati. 2019. Effect of Phytoremediation of Water Hyacinth (Eichornia crassipes) through Dilution on The Quality of Tofu Industry Effluent. LenteraBio 8(2): 113-119.
Wulandari, A., dan V. I Meitiniarti. 2021. Bioremediation of Pb and Cd contaminated soil using microorganisms. Journal of Science and Science Education 5(1): 1-11.