Resistance selection of local sorghum varieties in East Nusa Tenggara, Indonesia against Rhizoctonia solani

##plugins.themes.bootstrap3.article.main##

EWINDA ISENSI FENI
SRI KASMIYATI
V. IRENE MEITINIARTI

Abstract

Abstract. Feni EI, Kasmiyati S, Meitiniarti VI. 2023. Resistance selection of local sorghum varieties in East Nusa Tenggara, Indonesia against Rhizoctonia solani. Biodiversitas 24: 5309-5318. Sorghum is a multifunctional crop that can be used as an alternative food for the people of East Nusa Tenggara. However, one of the challenges in cultivating sorghum in this region is the presence of the Rhizoctonia solani pathogen. This fungus causes a devastating root rot disease in sorghum crops, resulting in loss of crop yields and potentially disrupting food security in the region. Using resistant varieties is one of the most effective ways to prevent the growth of R. solani biologically. Therefore, it is important to study the resistance of local sorghum varieties. The purpose of this study was to determine different sorghum varieties' resistance responses to the R. solani fungus that causes root rot disease. This study used a completely randomized design (CRD) with 2 levels of treatment: the treatment of sorghum varieties and the treatment of fungi inoculums, which were inoculated separately and simultaneously. Each treatment was repeated 3 times. Observations and data collection were carried out on the 7th day, and some of the parameters observed included germination percentage, radicle length, coleoptile length, sprout length, GTI value, and disease incidence value. The sorghum variety with the highest resistance value was found in the red variety. This red variety had a disease incidence value of 63.3%, much lower than the other four test varieties. These results provide important insights for developing a more root rot disease-resistant local sorghum plant variety.

##plugins.themes.bootstrap3.article.details##

References
Abdoulaye AH, Jia J, Abbas A, Hai D, Cheng J, Fu Y, Lin Y, Jiang D, and Xie J. 2022. Fusarivirus accessory helicases present an evolutionary link for viruses infecting plants and fungi. Virologica Sinica, 37(3), 427–436. DOI: https://doi.org/10.1016/j.virs.2022.03.010.
Akinci, I. E., dan Akinci, S. 2010. Effect of chromium toxicity on germination and early seedling growth in melons (Cucumis melon L.). African Journal of Biotechnology, 9 (29), 4589–4594.
Aydin MH. 2022. Rhizoctonia solani and Its biological control. Türkiye Tar?msal Ara?t?rmalar Dergisi, 9(1), 118–135. DOI: https://doi.org/10.19159/tutad.1004550.
Da Silva MP, Tylka GL, and Munkvold GP. 2017. Seed treatment effects on maize seedlings coinfected with Rhizoctonia solani and Pratylenchus penetrans. Plant Disease, 101(6), 957–963. DOI: https://doi.org/10.1094/PDIS-10-16-1417-RE.
Dewi OR, M MT, and Kulsum U. 2020. The effect of chitosan in suppressing the development of the sheath blight disease (Rhizoctonia solani khun) on rice (Oryza sativa l.). cropsaver - Journal of Plant Protection, 3(1), 8. DOI: https://doi.org/10.24198/cropsaver.v3i1.25535.
Ezward C, and Indrawanis E. 2019. Upaya peningkatan produktivitas sorgum (Sorghum bicolor L. Moench) melalui pupuk bioboost. Jurnal Ilmiah Pertanian. 16(1), 46–55. DOI: https://doi.org/10.31849/jip.v16i1.2344.
Guragain RP, Baniya HB, Pradhan SP, Pandey BP, Shrestha B, Fronczak M, Kierzkowska PH, and Subedi DP. 2023. Growth enhancement of radish seed induced by low-temperature argon plasma. Plasma Chemistry and Plasma Processing, 43(1), 111–137. DOI: https://doi.org/10.1007/s11090-022-10291-x.
Istikorini Y, dan Sari, O. Y. 2020. Survey dan identifikasi penyebab penyakit damping-off pada sengon (Paraserianthes falcataria) di persemaian permanen IPB. Jurnal Sylva Lestari, 8(1), 32–41.
Kabdwal BC, Sharma R, Kumar A, Kumar S, Singh KP, and Srivastava RM. 2023. Efficacy of different combinations of microbial biocontrol agents against sheath blight of rice caused by Rhizoctonia solani. Egyptian Journal of Biological Pest Control, 33(1), 1-18. DOI: https://doi.org/10.1186/s41938-023-00671-6.
Kalymbetov GY, Kedelbayev BS, Yelemanova ZR, and Sapargaliyeva B.2023. Effects of Different Biostimulants on Seed Germination of Sorghum Plants. Journal of Ecological Engineering, 24(3), 134–142. DOI: https://doi.org/10.12911/22998993/157568.
Kasmiyati S, Santosa S, Priyambada ID, Dewi K, and Sandradewi R. 2015. Perkecambahan biji dan pertumbuhan kecambah varietas sorgum (Sorghum bicolor L.) pada cekaman krom heksavalen. Bioma?: Berkala Ilmiah Biologi, 17(1), 41. DOI: https://doi.org/10.14710/bioma.17.1.41-54.
Koch E, Zink P, Bernhardt T, Birr T, and Linkies A. 2022. Location of pathogen inoculum in the potting substrate influences damage by Globisporangium ultimum, Fusarium culmorum and Rhizoctonia solani and effectiveness of control agents in maize seedlings. Agronomy, 12(6). DOI: https://doi.org/10.3390/agronomy12061388.
Koima IN, Kilalo DC, Orek CO, Wagacha JM, and Nyaboga EN. 2023. Identification and characterization of Colletotrichum Species causing sorghum anthracnose in Kenya and screening of sorghum germplasm for resistance to Anthracnose. Journal of Fungi, 9(1), 1–20. DOI: https://doi.org/10.3390/jof9010100.
Kumi F, Badji A, Mwila N, Odong T, Ochwossemakula M, Tusiime G, Gibson P, Biruma M, Prom LK, Cuevas HE, Agbahoungba S, and Rubaihayo P. 2019. New sources of sorghum resistant genotypes to downy mildew disease in Uganda. Biodiversitas, 20(11), 3391–3397. DOI https://doi.org/10.13057/biodiv/d201136.
Lamichhane JR, Dürr C, Schwanck AA, Robin MH, Sarthou JP, Cellier V, Messéan A, and Aubertot JN. 2017. Integrated management of damping-off diseases. A review. Agronomy for Sustainable Development, 37(2). DOI: https://doi.org/10.1007/s13593-017-0417-y.
Lone R, Hassan N, Bashir B, Khan G, and Ahmad N. 2023. Plant Stress Role of growth elicitors and microbes in stress management and sustainable production of Sorghum. Plant Stress, 9(March), 100179. https://doi.org/10.1016/j.stress.2023.100179.
Mayo-Prieto S, Rodríguez-González Á, Lorenzana A, Gutiérrez S, and Casquero PA. 2020. Influence of substrates in the development of bean and in pathogenicity of Rhizoctonia solani jg kühn. Agronomy, 10(5), 1–12. DOI: https://doi.org/10.3390/agronomy10050707.
Mofokeng MA, Shimelis H, Laing M, and Shargie N. 2017. Sorghum (Sorghum bicolor (L.) Moench) breeding for resistance to leaf and stalk anthracnose, Colletotrichum sublineolum, and improved yield: Progress and prospects. Australian Journal of Crop Science, 11(9), 1078–1085. DOI: https://doi.org/10.21475/ajcs.17.11.09.pne347.
Molla KA, Karmakar S, Molla J, Bajaj P, Varshney RK, Datta SK, and Datta K. (2020). Understanding sheath blight resistance in rice: the road behind and the road ahead. Plant Biotechnology Journal, 18(4), 895–915. DOI: https://doi.org/10.1111/pbi.13312.
Mukkun, L, Lalel HJD, and Kleden YL. 2021. The physical and chemical characteristics of several accessions of sorghum cultivated on drylands in east Nusa Tenggara, Indonesia. Biodiversitas, 22(5), 2520–2531. Doi: https://doi.org/10.13057/biodiv/d220509.
Nafriana, D. W., Indriyani, S., dan Prayogo, Y. 2013. Respon beberapa galur sorgum (Sorghum bicolor (L .) Moench) pada fase pertumbuhan vegetatif terhadap cendawan Rhizoctonia solani (Kuhn). Jurnal Biotropika, 1(3), 129–134.
Paiva CL, Netto DAM, Queiroz VAV, and Gloria MBA. 2022. Germinated sorghum (Sorghum bicolor L.) and seedlings show expressive contents of putrescine. Lwt-Food Science and Technologi 161 (2022) 113367 1–18 . DOI: https://doi.org/10.1016/j.lwt.2022.113367.
Pramono E, Kamal M, Susilo FX, and Timotiwu PB. 2018. Classification of seed resistance of various genotypes of sorghum (Sorghum bicolor [L.] Moench.) to weevil (Sitophilus sp.) during storage. Journal of Agronomy, 17(2), 81–92. DOI: https://doi.org/10.3923/ja.2018.81.91.
Rusae A, Metboki B, and Atini B. 2018. Identifikasi cendawan patogen pada tanaman sorgum di Timor Tengah Utara. Savana Cendana, 3(04), 69–71. DOI: https://doi.org/10.32938/sc.v3i04.463.
Singh SK, Patel MB, Thakker BN, Hooda KS, and Barad AK. 2019. Rhizoctonia solani.sp. sasakii inciting banded leaf and sheath blight of maize and their management: An Overview. International Journal of Current Microbiology and Applied Sciences, 8(07), 2858–2866. DOI: https://doi.org/10.20546/ijcmas.2019.807.356.
Sturrock CJ, Woodhall J, Brown M, Walker C, Mooney SJ, and Ray RV. 2015. Effects of damping-off caused by Rhizoctonia solani anastomosis group 2-1 on roots of wheat and oil seed rape quantified using X-ray computed tomography and real-time PCR. Frontiers in Plant Science, 6(JUNE), 1–11. DOI: https://doi.org/10.3389/fpls.2015.00461.
Sukto S, Lomthaisong K, Sanitchon J, Chankaew S, Falab S, Lübberstedt T, Lertrat K, and Suriharn K. 2021. Breeding for prolificacy, total carotenoids and resistance to downy mildew in small-ear waxy corn by modified mass selection. Agronomy, 11(9),1-17. DOI: https://doi.org/10.3390/agronomy11091793.
Tnunay IMY, Chikmawati T, and Miftahudin M. 2019. Morphological diversity of local sorghum cultivar (Sorghum bicolor) of East Nusa Tenggara, Indonesia. Biosaintifika: Journal of Biology & Biology Education, 11(1), 47–54. DOI: https://doi.org/10.15294/biosaintifika.v11i1.15199.
Xue CY, Zhou RJ, Li YJ, Xiao D, and Fu JF. 2018. Cell-wall-degrading enzymes produced in vitro and in vivo by Rhizoctonia solani, the causative fungus of peanut sheath blight. PeerJ, (9), 1-22. DOI: https://doi.org/10.7717/peerj.5580.
Yang Q, Yang L, Wang Y, Chen Y, Hu K,Yang W, Zuo S, Xu J, Kang Z, Xiao X, and Li G. 2022. A high-quality genome of Rhizoctonia solani, a devastating fungal pathogen with a wide host range. Molecular Plant-Microbe Interactions?: MPMI, 35(10), 954–958. DOI: https://doi.org/10.1094/MPMI-06-22-0126-A.
Yellareddygari S, Reddy MS and Kloepper. J. 2014. Rice sheath blight: A review of disease and pathogen management approaches. Journal of Plant Pathology and Microbiology, 05(04), 3–7.DOI: https://doi.org/10.4172/2157-7471.1000241.
Yulia E, Widiantini F. 2018. Ekstrak tanaman binahong sebagai pengendali penyakit hawar pelepah daun padi. Jurnal Fitopatologi Indonesia, 14(4), 138. DOI: https://doi.org/10.14692/jfi.14.4.138.
Yulianti T, Suhara C, dan Hidayah, N. 2016. Pengaruh inkubasi bahan organik yang diperkaya dengan mimba terhadap keparahan penyakit rebah kecambah. Buletin Tanaman Tembakau, Serat dan Minyak Industri, 4(2), 68. DOI: https://doi.org/10.21082/bultas.v4n2.2012.68-75.
Zubair A. (2018). SORGUM - Tanaman Multi Manfaat. Unpad Press. Bandung.