The phenetic relationship of citrus plants based on the morphological and anatomical characteristics

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MOHAMAD NURZAMAN
TIA SETIAWATI
RUSDI HASAN
NAZLA KARUNIA QOTRUNNADA
JOKO KUSMORO
NANDANG PERMADI
EUIS JULAEHA

Abstract

Abstract. Nurzaman M, Setiawati T, Hasan R, Qotrunnada NK, Kusmoro J, Permadi N, Julaeha E. 2024. The phenetic relationship of citrus plants based on the morphological and anatomical characteristics. Biodiversitas 25: 1201-1213. Indonesia stands as a global biodiversity hub, boasting an exceptional richness that earns it the distinction of being a mega-biodiversity country. Among its diverse ecosystems, the Rutaceae family holds significance, encompassing numerous genera, notably the Citrus genus, which showcases a splendid array of citrus plants. This research aimed to determine phenetic relationships based on the morphological and anatomical characters of seven citrus plants (Citrus reticulata var. Chokun, C. reticulata var. Fremont, C. reticulata var. Santang, C. reticulata var. Dekopon, C. aurantium var. variegata, C. aurantium, and C. limon) in Parongpong District, West Bandung Regency, West Java. The method used in this research was explorative with purposive sampling. This study collected three samples for respective citrus plants for character observations of leaf morphology, anatomy, and fruit morphology. Morphology and anatomy observations were conducted in the Department of Biology, Padjadjaran University laboratory. The description of morphological and anatomical data obtained from each orange plant was then analyzed using NTSYS PC version 2.11a software to obtain similarity values using the dendrogram analysis method. Phenetic analysis of seven orange plants produced a dendrogram divided into 2 main branches, branch I and branch II, with a similarity coefficient of 0.3846. Branch I consists of Chokun orange (Citrus reticulata var. Chokun), Fremont orange (C. reticulata cv. Fremont), Santang orange (C. reticulata cv. Santang), and variegated orange (C. sinensis cv. variegata) and branch II consists of dekopon orange (C. reticulata var. Dekopon), Sunkist orange (C. aurantium), and lemon (C. limon).

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References
Adlini MN, Umaroh HK. 2021. Karakterisasi tanaman jeruk (Citrus sp.) di Kecamatan Nibung Hangus Kabupaten Batu Bara Sumatera Utara. KLOROFIL: Jurnal Ilmu Biologi dan Terapan. 4 (1). DOI: 10.30821/kfl:jibt.v4i1.8921.
Andrini A, Martasari C, Budiyati E. 2021. Teknologi Inovatif Jeruk Sehat Nusantara. Bogor: IPB Press.
Bashir K, Sohail A, Ali U, Ullah A, Ul Haq Z, Gul B, Ullah I, Sunera, Asghar M. 2020. Foliar micromorphology and its role in identification of the Apocynaceae taxa. Microsc Res Tech. 83 (7). DOI: 10.1002/jemt.23466.
Boruah P, Jamja T, Dhar S. 2023. Socio-Economic Impact of Horticulture?: Empowering New Horizons in Horticulture Revolutionizing Agricultural Practices. (November):1–36.
Buckley TN, John GP, Scoffoni C, Sack L. 2017. The sites of evaporation within leaves. Plant Physiol. 173 (3): 1763–1782. DOI: 10.1104/pp.16.01605.
Cabrera LP, Etxeberria E, Gonzalez P, Reyes TF. 2023. Use of non-intrusive laser exfoliation to improve substance uptake into citrus leaves [version 3?; peer review?: 2 approved ]. (May):1–16.
Castle WS, Krezdorn AH. 2022. Anatomy and morphology of field-sampled citrus fibrous roots as influenced by sampling depth and rootstock1. HortScience. 14 (5). DOI: 10.21273/hortsci.14.5.603.
Chan SROS, Achmad BS, Ferdinant. 2021. Morphological characterization of gunung omeh citrus (Citrus nobilis Lour) in Guguak District, Lima Puluh Kota Regency. IOP Conf Series: Earth and Environmental Science. 1097: 012032. DOI: 10.1088/1755-1315/1097/1/012032
Correa-Marinez A, Grenabo J, Bock D, Wedin A, Angenete E. 2018. The type of stoma matters—morbidity in patients with obstructing colorectal cancer. Int J Colorectal Dis. 33 (12). DOI: 10.1007/s00384-018-3164-x.
Couvreur TLP, Dauby G, Blach-Overgaard A, Deblauwe V, Dessein S, Droissart V, Hardy OJ, Harris DJ, Janssens SB, Ley AC. 2021. Tectonics, climate and the diversification of the tropical African terrestrial flora and fauna. Biological Reviews. 96 (1): 16–51. DOI: 10.1111/brv.12644.
Feng L, Raza MA, Li Z, Chen Y. 2019. The influence of light intensity and leaf movement on photosynthesis characteristics and carbon balance of soybean. 9 (January):1–16. DOI: 10.3389/fpls.2018.01952.
Garvita RV, Wawangningrum H. 2020. Stomata cells studies of Paraphalaenopsis spp. from in vitro and greenhouse condition. Biodiversitas. 21 (3). DOI: 10.13057/biodiv/d210335.
Glime JM. 2017. Water Relations: Leaf Strategies – Cuticles and Waxes. In: Glime, J M Bryophyte Ecology Volume 1 Physiological Ecology. Vol. 1. [place unknown]; p. 1–14.
Habiba, Sarkar D, Haque MdE, Islam MdA, Mukherjee A, Sikda B. 2019. Assessment of genetic diversity and phenetic relationships among some Bangladeshi cultivars of cucumber (Cucumis sativus L.) using isozyme and protein profiling. Vegetos. 32:494–507. DOI: 10.1007/s42535-019-00065-w.
Hanif Z, Agency I, Ashari H, Agency I, Honestin T, Agency I. 2021. Pascapanen dan Pengolahan Hasil Jeruk. [place unknown].
Hoshino R, Yoshida Y, Tsukaya H. 2019. Multiple steps of leaf thickening during sun-leaf formation in Arabidopsis. Plant J. 100 (4): 738–753. DOI: 10.1111/tpj.14467.
IPGRI. 1999. Descriptors for Citrus (Citrus spp.). Rome, Italy.: International Plant Genetic Resources Institute.
Jentzsch M, Becker S, Thielen M, Speck T. 2022. Functional anatomy, impact behavior and energy dissipation of the peel of Citrus × limon: A comparison of Citrus × limon and Citrus maxima. Plants. 11 (7). DOI: 10.3390/plants11070991.
Julaeha E, Pandiangan NS, Eddy DR, Permadi N, Harja A, Wahyudi T, Al-Anshori J. 2023. Ethyl cellulose-based microcapsules of Citrus aurantifolia (Christm.) Swingle essential oil with an optimized emulsifier for antibacterial cosmetotextiles. Polymer (Guildf). 283:126265. DOI: 10.1016/j.polymer.2023.126265.
Kandowangko NY, Febriyanti. 2023. Genetic diversity of the genus Citrus in Tomini Bay coastal areas, Indonesia based on morphological characters. Biodiversitas. 24 (5). DOI: 10.13057/biodiv/d240549.
Kane CN, Jordan GJ, Jansen S, McAdam SAM. 2020. A permeable cuticle, not open stomata, is the primary source of water loss from expanding leaves. Front Plant Sci. 11. DOI: 10.3389/fpls.2020.00774.
Kerstiens G. 1996. Plant cuticles — an integrated functional approach. J Exp Bot. 47:50–60.
Kim S, Choi SK, Van S, Kim ST, Kang YH, Park SR. 2022. Geographic differentiation of morphological characteristics in the brown seaweed Sargassum thunbergii along the Korean coast: A response to local environmental conditions. J Mar Sci Eng. 10 (4). DOI: 10.3390/jmse10040549.
Kosmiatin M, Martasari C, Amelia Kurniawati F, Gandhi Ningrum Prasetia R, Sari Dewi P. 2019. Analysis of stomata, chloroplast, and chromosome of local mandarin Citrus (Citrus reticulata) plants grown from endosperm culture. In: IOP Conf Ser Earth Environ Sci. Vol. 406. DOI: 10.1088/1755-1315/406/1/012010.
Kumari S, Bhowal R, Suprasanna P. 2023. Sustainable approaches for biodiversity and bioprospecting of Citrus. Sustainability (Switzerland). 15 (9): 1–21. DOI: 10.3390/su15097731.
Lin PA, Chen Y, Ponce G, Acevedo FE, Lynch JP, Anderson CT, Ali JG, Felton GW. 2022. Stomata-mediated interactions between plants, herbivores, and the environment. Trends Plant Sci. 27 (3). DOI: 10.1016/j.tplants.2021.08.017
Manasherova E. 2021. The plant cuticle?: An ancient guardian barrier set against long-standing rivals. 12(June). DOI: 10.3389/fpls.2021.663165.
McAdam SAM, Brodribb TJ. 2018. Mesophyll cells are the main site of abscisic acid biosynthesis in water-stressed leaves. Plant Physiol. 177 (3): 911–917. DOI: 10.1104/pp.17.01829.
Muntoreanu T, Cruz R, Melo-de-Pinna G. 2011. Comparative leaf anatomy and morphology of some neotropical Rutaceae: Pilocarpus vahl and related genera. Plant Syst Evol. 296: 87–99. DOI: 10.1007/s00606-011-0478-3.
Nurzaman M, Permadi N, Setiawati T, Hasan R, Irawati Y, Julaeha E, Herlina T. 2022. DPPH free radical scavenging activity of Citrus aurantifolia Swingle peel extracts and their impact in inhibiting the browning of Musa paradisiaca L. var. Kepok Tanjung explants. Jordan J Biol Sci. 15: 771–777. DOI: 10.54319/jjbs/150505.
Ogundare CS, Saheed SA. 2012. Foliar epidermal characters and petiole anatomy of four species of Citrus L. (Rutaceae) from South-Western Nigeria. Bangladesh J Plant Taxon. 19 (1). DOI: 10.3329/bjpt.v19i1.10938.
De Oliveira AC, Novac Garcia A, Cristofani M, Machado MA. 2002. Identification of citrus hybrids through the combination of leaf apex morphology and SSR markers. Euphytica. 128 (3). DOI: 10.1023/A:1021223309212.
Opara U, Studman C, Banks N. 2010. Fruit skin splitting and cracking. In: Hort Rev. Vol. 19.; p. 217–262. DOI: 10.1002/9780470650622.ch4.
Pautov A, Bauer S, Ivanova O, Krylova E, Yakovleva O, Sapach Y, Pautova I. 2021. Stomatal rings: Structure, functions and origin. Bot J Linn So. 195 (3). DOI: 10.1093/botlinnean/boaa096.
Permadi N, Julaeha E, Rosandi Y, Nurzaman M. 2021. Antioxidant activity of non-volatile lime (Citrus aurantifolia Swingle) extract. Jurnal Agrinika?: Jurnal Agroteknologi dan Agribisnis. 5: 122. DOI: doi.org/10.30737/agrinika.v5i2.1919.
Permadi N, Nurzaman M, Alhasnawi AN, Doni F, Julaeha E. 2023. Managing lethal browning and microbial contamination in Musa spp. tissue culture: Synthesis and perspectives. Horticulturae. 9 (4). DOI: 10.3390/horticulturae9040453.
Pratiwi Y, Agustin HY. 2021. Development of morphological characterization magazine of Terigas tangerins (Citrus reticulata Blanco) at Mekarsari citrus picking tour, Blitar Regency. Jurnal Ilmiah Biosmart. 7: 1–17. DOI: 10.30872/jibs.v7i2.658.
Purnama SY, Chatri M, Selaras GH. 2021. Stomata characteristics of several type of Sansevieria. Serambi Biologi. 6 (1).
Qi Z, Pi E, Zhang X, Möller M, Jiang B, Lu H. 2017. Ontogenesis of D-type stomata and cork-warts on the leaf epidermis of Camellia japonica (Theaceae) and functional assessment. Flora: Morphology, Distribution, Functional Ecology of Plants. 228. DOI: 10.1016/j.flora.2017.01.010.
Rahman IU, Afzal A, Calixto ES, Iqbal Z, Abdalla M, Alsamadany H, Parvez R, Romman M, Ali N, Sakhi S, Shah M. 2022. Species-specific and altitude-related variations in stomatal features of Berberis lycium Royle and B. parkeriana C.K. Schneid. Bot Lett. 169 (1). DOI: 10.1080/23818107.2021.1947371.
Rezai S, Etemadi N, Nikbakht A, Yousefi M. 2006. Effect of light intensity on leaf morphology, photosynthetic capacity, and chlorophyll content in sage (Salvia officinalis L.). 46–57.
Rohlf F. 1988. NTSYS-pc - Numerical Taxonomy and Multivariate Analysis System. Applied Biostatistics Inc New York. 2.1.
Roman-Benn A, Contador CA, Li M-W, Lam H-M, Ah-Hen K, Ulloa PE, Ravanal MC. 2023. Pectin: An overview of sources, extraction and applications in food products, biomedical, pharmaceutical and environmental issues. Food Chem Adv. 2:100192. DOI: 10.1016/j.focha.2023.100192.
Schmid, R., Harris, J. G., & Harris MW. 2022. Plant Identification Terminology: An Illustrated Glossary. Taxon. 51(3).
Schrader J, Shi P, Royer D, Peppe D, Gallagher R, Li Y, Wang R, Wright I. 2021. Leaf size estimation based on leaf length, width and shape. Ann Bot. DOI: 10.1093/aob/mcab078.
Seyedi FS, Nafchi MG, Reezi S. 2024. Effects of light spectra on morphological characteristics, primary and specialized metabolites of Thymus vulgaris L. Heliyon. 10(1):e23032. DOI: 10.1016/j.heliyon.2023.e23032.
Shi P, Zhao L, Ratkowsky DA, Niklas KJ, Huang W, Lin S, Ding Y, Hui C, Li B-L. 2019. Influence of the physical dimension of leaf size measures on the goodness of fit for Taylor’s power law using 101 bamboo taxa. Glob Ecol Conserv. 19:e00657. DOI: 10.1016/j.gecco.2019.e00657.
Shtein I, Shelef Y, Marom Z, Zelinger E, Schwartz A, Popper ZA, Bar-On B, Harpaz-Saad S. 2017. Stomatal cell wall composition: Distinctive structural patterns associated with different phylogenetic groups. Ann Bot. 119(6). DOI: 10.1093/aob/mcw275.
Sofiyanti N, Iriani D, Wahyuni PI, Idani N, Lestari P. 2022a. Identification, morphology of Citrus L. (Aurantioideae-Rutaceae Juss.) and its traditional uses in Riau Province, Indonesia. Biodiversitas. 23(2). DOI: 10.13057/biodiv/d230247.
Sofiyanti N, Wahyuni PI, Iriani D. 2022b. Stomatal characteristics of 5 Citrus L. species (Rutaceae) From Pekanbaru, Riau Province. Jurnal Biologi Tropis. 22 (1). DOI: 10.29303/jbt.v22i1.3100.
Suratman, Suranto, Muzzazinah, Purnomo. 2022. Leaf anatomical characters variation of Strobilanthes s.l. from Sumatra, Indonesia and its taxonomic implications. Biodiversitas. 23 (7): 3706–3721. DOI: 10.13057/biodiv/d230748.
Thompson S, Clinton M, Miller B, Fu Z. 2023a. Methods for viewing plant stomata responses. Am Biol Teach. 85 (1): 33–37. DOI: 10.1525/abt.2023.85.1.33.
Tian M, Yu G, He N, Hou J. 2016. Leaf morphological and anatomical traits from tropical to temperate coniferous forests: Mechanisms and influencing factors. Sci Rep. 6:19703. DOI: 10.1038/srep19703.
Tuasamu Y. 2018. Characterization of stomato leaf and anatomic morphology in several species of orange plants (Citrus sp.). AGRIKAN Jurnal Agribisnis Perikanan. 11 (2): 85–90. DOI: 10.29239/j.agrikan.11.2.85.
Tuwo M, Kuswinanti T, Nasruddin A, Tambaru E. 2023. Diverse morphology and anatomy of Citrus Spp. (Orange) in South Sulawesi, Indonesia plantations: A comprehensive study. Pa J Biol Sci. 26 (6): 321–333. DOI: 10.3923/pjbs.2023.321.333.
UNEP. 2018. TEEB Implementation in Indonesia: “Promoting biodiversity and sustainability in the agriculture and food sector project” A background review of agriculture in Indonesia.
Vialet-Chabrand S, Matthews JSA, Simkin AJ, Raines CA, Lawson T. 2017. Importance of fluctuations in light on plant photosynthetic acclimation. Plant Physiol. 173 (4): 2163–2179. DOI: 10.1104/pp.16.01767.
Yu Haibin, Yang M, Lu Z, Wang W, Yu F, Zhang Y, Yin X, Yu Hongjun, Hu J, Deane DC. 2023. A phylogenetic approach identifies patterns of beta diversity and floristic subregions of the Qinghai-Tibet Plateau. Plant Divers. DOI: 10.1016/j.pld.2023.07.006.
Yuan B, Ding G, Ma J, Wang L, Yu L, Ruan X, Zhang X, Zhang W, Wang X, Xie Q. 2020. Comparison of morphological characteristics and determination of different patterns for rubber particles in dandelion and different rubber grass varieties. Plants. 9 (11). DOI: 10.3390/plants9111561.
Zahara M, Win CC. 2019. Morphological and stomatal characteristics of two Indonesian local orchids. J Trop Hortic. 2 (2). DOI: 10.33089/jthort.v2i2.26.
Zhu X, Zhang Y, Du Z, Chen X, Zhou X, Kong X, Sun W, Chen Z, Chen C, Chen M. 2018. Tender leaf and fully-expanded leaf exhibited distinct cuticle structure and wax lipid composition in Camellia sinensis cv. Fuyun 6. Sci Rep. 8 (1): 14944. DOI: 10.1038/s41598-018-33344-8.

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