Antibiotic production by an endophytic Streptomyces isolated from the medicinal plant Poikilospermum suaveolens
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ALIMUDDIN ALI
♥
MUHARRAM PASSITTA
https://orcid.org/0000-0002-0459-1931
HERLINA RANTE
https://orcid.org/0000-0003-4063-2208
ELLY WAHYUDIN
https://orcid.org/0000-0002-4602-787X
NANA JUNIARTI NATSIR DJIDE
https://orcid.org/0000-0001-5495-0958
ROLAND JANSEN POLITAN
https://orcid.org/0009-0000-5489-5792
ELYZA AIMAN NUR
https://orcid.org/0009-0004-2608-4748
SATORU SHIGENO
https://orcid.org/0009-0006-6528-8975
SATOSHI OHTE
https://orcid.org/0000-0002-5153-2176
KEISUKE KOBAYASHI
https://orcid.org/0000-0001-9580-2484
KANJI HOSODA
https://orcid.org/0009-0003-4025-8348
HIROSHI TOMODA
https://orcid.org/0000-0002-1241-8901
TAICHI OHSHIRO
https://orcid.org/0000-0003-3609-0717
Abstract
Abstract. Ali A, Passitta M, Rante H, Wahyudin E, Djide NJN, Politan RJ, Nur EA, Shigeno S, Ohte S, Kobayashi K, Hosoda K, Tomoda H, Ohshiro T. 2024. Antibiotic production by an endophytic Streptomyces isolated from the medicinal plant Poikilospermum suaveolens. Biodiversitas 25: 2221-2229. This study investigates the antibiotic production potential of endophytic actinomycetes isolated from Poikilospermum suaveolens plants. Samples were collected from various parts of the plant in karst ecosystems. Endophytic actinomycetes were cultured and screened for antimicrobial activity using agar diffusion and liquid microdilution methods. One of the 11 isolated strains exhibited significant antibiotic activity against clinically relevant organisms identified as belonging to the Streptomyces genus, specifically Streptomyces mirabilis strain NRRL ISP-5169. Submerged fermentation of this strain over 9 days showed strong antagonistic effects against Gram-positive bacteria. Spectroscopic analyses confirmed the presence of actinomycin D as the bioactive compound. This study provides on the previously unexplored potential of endophytic actinomycetes from P. suaveolens as a promising resource for antibiotic discovery.
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