Microbial quality and macronutrient of vermicompost produced by Eisenia fetida in dairy wastewater solids




Abstract. Marlina ET, Hidayati YA, Harlia E, Badruzzaman DZ, Meynadhea N, Rahayu NA. 2024. Microbial quality and macronutrient of vermicompost produced by Eisenia fetida in dairy wastewater solids. Biodiversitas 25: 2729-2737. Dairy Wastewater Solids (DWS) contain enough nutrients for the growth of earthworms and utilize the nutritional requirements of livestock waste for growth. The growth of earthworms affects the quality of vermicompost or castings that are useful as organic fertilizers. This research aimed to determine the growth of Eisenia fertida using DWS as a growth medium, and its effect on the quality of vermicompost produced. The organic material decomposed in DWS by indigenous microorganisms and earthworms can convert DWS into organic fertilizer through composting. Rice straw was added as a carbon source to obtain the ideal C/N ratio for the growth of decomposing microorganisms. Good growth of decomposer microorganisms can increase the availability of nutrients in DWS as a source of plant nutrition. E. fetida was used in vermicomposting for 14 days after the initial decomposition phase had passed. This research used the experimental method of Completely Randomized Design (CRD) with three stocking density treatments (T1: 6.6 g/L; T2: 13.3 g/L; T3: 20.0 g/L ) and six replications. The results showed that higher stocking density promoted an increase in the biomass weight of earthworms. Various indigenous microorganisms act as decomposers and work with earthworms to convert organic matter into inorganic matter as a plant nutrient. The stocking density treatment had no significant effect on vermicompost production, total nitrogen content, and P2O5 vermicompost but had a significant effect on K2O, Ca, Mg, population of Nitrogen-Fixing Bacteria (NFB) and Phosphate-Solubilizing Bacteria (PSB). Vermicomposting, DWS, and rice straw mixture produced vermicompost with high functional microbial and macronutrient concentrations.


Aguilar-Paredes, A., G. Valdes, N. Araneda, E. Valdebenito, F. Hansen, M. Nuti. 2023. Microbial Community in the Composting Process and Its Positive Impact on the Soil Biota in Sustainable Agriculture. Agronomy 13 (2) :1-24, DOI: 10.3390/agronomy13020542
Agustina, U.P., A. Prasetya, and A. Pertiwiningrum. 2018. Optimization of the Use of Farm Waste and Water Hyacinth for Earthworm (Lumbricus rubellus) Cultivation Media. Bulletin of Animal Science. Buletin Peternakan 42 (2): 157-163, DOI: 10.21059/buletinpeternak.v42i2.31581
Azis, F.A., M. Choo, H. Suhaimi, Pg.E. Abas. 2023. The Effect of Initial Carbon to Nitrogen Ratio on Kitchen waste Composting Maturity. Sustainability 2023, 15 (7): 1-18, DOI: 10.3390/su15076191
Devi, J., R. Pegu, H. Mondal, R. Roy, S. S. Bhattacharya. 2023 Earthworm stocking density regulates microbial community structure and fatty acid profiles during vermicomposting of lignocellulosic waste: Unraveling the microbe-metal and mineralization-humification interactions. Bioresource Tech. 367: 1-11. DOI: 10.1016/j.biortech.2022.128305
Dume, B., A. Hanc, P. Svehla, P. Michal, A. D. Chane, A. Nigussie. 2023. Composting and vermicomposting of sewage sludge at various C/N ratios: Technological feasibility and end-product quality. Ecotoxycology and Environmental Safety 263: 1-11, DOI: 10.1016/j.ecoenv.2023.115255
El Jawaher A. Bin Dohaish, 2020. Vermicomposting of organic waste with Eisenia fetida increases the content of exchangeable nutrients in soil. Pak. J. Biol. Sci., 23 (4): 501-509. DOI: 10.3923/pjbs.2020.501.509
Hadija, T. Kuswinanti, M.Jayadi and S.H Larekeng. 2021. Isolation, characterization and identification of nitrogen fixing bacteria with organic fertilizer applications in paddy soil. IOP Conf. Series: Earth and Environmental Science 807 : 1-7, DOI: 10.1088/1755-1315/807/2/022024
Hannah M.J. Vos, Tjisse Hiemstra, Miguel Prado Lopez, Jan Willem van Groenigen, Andreas Voegelin, Stefan Mangold, Gerwin F. Koopmans. 2023. Earthworms affect reactive surface area and thereby phosphate solubility in iron-(hydr)oxide dominated soils. Geoderma 428:1-9, DOI: 10.1016/j.geoderma.2022.116212
Hu, Y., B.J. Jin, X.P. Liu, X.Y. Li, W.F. Wu, X.T. Wang, C. Sun, G. Li, Y.G. Zhu, X. Lin. 2024. Earthworms Facilitate Soil Organic Phosphorus Mineralization Primarily by Their Gut Microbiome. SSRN: https://ssrn.com/abstract=4704827 or http://dx.doi.org/10.2139/ssrn.4704827
Ikhwani, N. Saribanon, T. Mitra Setia, E. Yuniarti, Jumakir. 2021. Nitrogen-fixation and phosphate-solubilization bacteria isolated from alluvial and latosol soil paddy field. BIODIVERSITAS 22 (11): 4722-4730 DOI: 10.13057/biodiv/d221103
Janati, W., R. Bouabid, K. MikouL, L. El Ghadraoui, F. Errachidi. 2023. Phosphate solubilizing bacteria from soils with varying environmental conditions: Occurrence and function. PLoS ONE 18(12): 1 - 14, DOI: 10.1371/journal.pone.0289127
Jesna, M., and A. R. Livingstone. 2023. Effect of temperature on regeneration of Eisenia fetida. Int. J. of Engineering Tech. and Management Sciences. 7 (3): 52-55. DOI:10.46647/ijetms.2023.v07i04.011
Jose, J.V. 2023. Chapter one - Physologcal and molecular aspects of macronutrent uptake by higher plants, Sustanable Plant Nutrition, Molecular Interventions and Advancements for Crop Improvement, 2023, p 1-21, DOI: 10.1016/B978-0-443-18675-2.00010-9
Khan, H., W. A. Akbar, Z. Shah, H. Ur. Rahim, A. Taj, J.M. Alatalo. 2022. Coupling phosphate-solubilizing bacteria (PSB) with inorganic phosphorus fertilizer improves mungbean (Vigna radiata) phosphorus acquisition, nitrogen fixation, and yield in alkaline-calcareous soil. Heliyon 8 (3): 1-10, DOI: 10.1016/j.heliyon.2022.e09081
Minister of Agriculture of the Republic of Indonesia. 2019. Regulation of the Minister of Agriculture of the Republic of Indonesia Number 01 of 2019.
Niazi, P and A.W. Monib. 2023. Function of Macronutrients in Plant Growth and Human. Int. J. of Scientific Development and Research, 2023, 8 (4) : 1265-1274. DOI: 10.1729/Journal.33883
Ndegwa, P.M., S.A. Thompson, K.C. Das. 2000. Effect of Stocking density and feeding rate on vermicomposting of biosolids. Bioresource Tech. 71 (1) : 5-12. DOI: 10.1016/S0960-8524(99)00055-3
Papale, M., I. Romano, I. Finore, A.L. Giudice, A. Piccolo, S. Cangemi, V.D. Meo, B. Nicolaus, A. Poli. 2021. Prokaryotic Diversity of the Composting Thermophilic Phase: The Case of Ground Coffee Compost. Microorganisms 218, 9(2) : 1-19, DOI: 10.3390/microorganisms9020218
Putri, I.D., D. Indrawati, R. Ratnaningsih. 2020. The Effect of Worm Density in Vermicomposting of Vegetable Waste and Cow Manure Using Lumbricus rubellus. Int, J. of Sci. & Tech. Research, Vol. 9, Issue 03, p. 166-170.
Raimondo, M., G. D. R. Simeone, G. P. Coppola, M. Zaccardelli, F. Caracciolo, M. A. Rao. 2023. Economic benefits and Soil improvement: Impacts of vermicompost use in spinach production through industrial symbiosis. Journal of Agriculture and Food Research 14:1-8, DOI: 10.1016/j.jafr.2023.100845
Raza, S.T., Z. Bo, Z. Ali and T. J. Liang. 2019. Vermicomposting by Eisenia fetida is a Sustainable and Eco-Friendly Technology for Better Nutrient Recovery and Organic WasteManagement in Upland Areas of China. Pakistan J. Zool. 51(3) : 1027-1034, DOI: 10.17582/journal.pjz/2019.51.3.1027.1034
Ramnarain, Y.I., A.A. Ansari, L. Ori. 2018. Vermicomposting of different organic materials using the epigeic earthworm Eisenia foetida. International Journal of Recycling of Organic Waste in Agriculture 8:23–36, DOI: 10.1007/s40093-018-0225-7
Shafique, I., S. Andleeb, F. Naeem, S. Ali, T. Tabassam, T. Sultan, M. A. Abbasi. 2023. Cow dung putrefaction via vermicomposting using Eisenia fetida and its influence on seed sprouting and vegetative growth of Viola wittrockiana (pansy). PLoS ONE 18(2): 1-18, DOI: 10.1371/journal.pone.0279828
Timofeeva, A.; Galyamova, M.; Sedykh, S. 2022. Prospects for Using Phosphate-Solubilizing Microorganisms as Natural Fertilizers in Agriculture. Plants 11 (16): 1-23, DOI: 10.3390/plants11162119
Thomas, J., Sajeshkumar N.K, Jiby John Mathew and Prem Jose Vazhacharickal 2021. Isolation of nitrogen fixing, phosphate solubilizing bacteria and development of biofertilizer for crop improvement First Edition – January 2021 ISBN 9798596254617 ISBN 9798596254617
Tortosa, G., A.J. Fernandez-Gonzalez, A.V. Lasa, E. Aranda, F. Torralbo, C. Gonzalez-Murua, M. Fernandez-Lopez, E. Benitez, E. J. Bedmar. 2021. Involvement of the metabolically active bacteria in the organic matter degradation during olive mill waste composting. Science of the Total Environment 789: 1-10, DOI: 10.1016/j.scitotenv.2021.147975
Viljoen, S.A and A.J. Reinecke. 1992. The temperature requirements of the epigeic earthworm species Eudrilus eugeniae (Oligochaeta)-a laboratory study. Soil Biology and Biochemistry 24 (12): 1345-1250, DOI: 10.1016/0038-0717(92)90116-F
Yakkou, L., S. Houida, M. Chelkha, S. Bilen, M. Raouane, S. Amghar, A. El Harti. 2024. Chapter 2- How do earthworms affect the microbial community during vermicomposting for organic waste recycling? Earthworm Tech. in Organic Waste Management, Waste and the Environment: Underlying Burdens and Management Strategies, p. 15-39, DOI: 10.1016/B978-0-443-16050-9.00032-3
Zhang X, Tong J, Dong M, Akhtar K, He B. 2022. Isolation, identification and characterization of nitrogen fixing endophytic bacteria and their effects on cassava production. PeerJ 10:1 - 21, DOI: 10.7717/peerj.12677.

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