Natural association of the entomopathogenic fungi Aschersonia placenta with spiralling whitefly (Aleurodicus dugesii) in Bali, Indonesia
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Abstract. Sudiarta IP, Suputra IPW, Mertaningsih NP, Wirya GNAS, Selangga DGW, Utama IWEK, Gargita IWD, Klett K, Yudha IKW. 2024. Natural association of the entomopathogenic fungi Aschersonia placenta with spiralling whitefly (Aleurodicus dugesii) in Bali, Indonesia. Biodiversitas 25: 4067-4073. The whitefly is one of the most common pests in agriculture, and one highly polyphagous species is the spiral whitefly (Aleurodicus sp.). The fungal genus Aschersonia can be used to control Aleurodicus sp.. This research has potential applications in agriculture because it can produce a new environmentally friendly method to control whitefly infestations. The aim of this study was to analyze the morphology and molecular characteristics of Aschersonia and their hosts in Bali. The samples were taken from several areas in Bali Province. Morphological identification was carried out at the Plant Pathology Laboratory, Faculty of Agriculture, Universitas Udayana. The conidia of Aschersonia were fusoid with tapered ends, 9-16 ?m long and 1.5-2 ?m wide, and the color of the culture was white to yellowish white. Molecular analysis exhibited that DNA bands measuring between 500-600 bp were successfully amplified with universal primers ITS1/ITS4. Aschersonia infected spiralling whiteflies has never been reported in mulberry plants. Further phylogenetic analysis showed that Aschersonia-infected spiralling whiteflies were in the same group as Aschersonia placenta/ Hypocrella raciborskii isolates from Thailand and India. Aleurodicus dugesii Cockerell 1896 had morphological characteristics, such as vasiform orifice with lingula extending beyond the borders of orifice, compound pores present on puparia, and thoracic legs with claws. The results of morphological analysis showed that A. dugesii from Bali (LC491422) had the closest kinship to A. dugesii from the USA (AY521251). This is the first report of the identification of Aschersonia placenta associated with A. dugesii in Indonesia.
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References
Francis AW, Stocks IC, Smith TR, Boughton AJ, Mannion CM, Osborne LS. 2016. Host plants and natural enemies of rugose spiraling whitefly (Hemiptera: Aleyrodidae) in Florida. Florida Entomol 99 (1): 150-153. DOI: 10.1653/024.099.0134.
Gregory S, Hodges, Evans GA. 2005. An identification guide to the whiteflies (Hemiptera: Aleyrodidae) of the southeastern United States. Florida Ent 88 (4): 518-534. DOI: 10.1653/0015-4040(2005)88[518:AIGTTW]2.0.CO;2.
Hidayat P, Bintoro D, Nurulalia L, Basri M. 2018. Species identification, host range, and identification key of whiteflies of Bogor and surrounding area. J HPT Trop 18 (2): 127-150. DOI: 10.23960/j.hptt.218127-150.
Hidayat P, Dhango VH, Nurulalia L, Nurulita S. 2023. Characterization and identification of two economically important whitefly species, genus Aleurodicus, subfamily Aleurodicinae (Hemiptera: Aleyrodidae) from Western Java, Indonesia. Serangga 28 (2): 28-39. DOI: 10.17576/serangga-2023-2802-03.
Hidayat P, Maryana E, Kusumah YM, Nurulaila L. 2020. Host range and population density of the giant whitefly Aleurodicus dugesii Cockerell (Hemiptera: Aleyrodidae) on horticultural crops in Cipanas-Cianjur, West Java, Indonesia. IOP Conf Ser: Earth Environ Sci 468: 012008. DOI: 10.1088/1755-1315/468/1/012008.
Ingle YV, Bhosale DN, Karande VD, Bramhankar SB, Mane SS, Paithankar DH, Sadawarte AK. 2022. Identification, pathogenesis and compatibility of Aschersonia aleyrodis (Webber) with selected fungicides and insecticides. Intl J Trop Insect Sci 42: 2093-2101. DOI: 10.1007/s42690-022-00730-9.
Kajita H, Samudra IM, Naito A. 1991. Discovery of the spiraling whitefly Aleurodicus dispersus Russell (Homoptera: Aleyrodidae) from Indonesia, with notes on its host plants and natural enemies. Appl Ent Zool 26 (3): 397-400. DOI: 10.1303/aez.26.397.
Kumashiro BR, Lai PY, Funasaki GY, Teramoto KK. 1983. Efficacy of Nephaspis amnicola and Encarsia haitiensis in controlling Aleurodicus dispersus in Hawaii. Proc Hawaiian Entomol Soc 24: 261-269.
Kurniawan HA, Fitria F. 2021. Life balance of whitefly (Bemisia tabaci Genn.) (Hemiptera: Aleyrodidae) in chili plant (Capsicum annuum L.). Agrinula 4 (1): 22-26. DOI: 10.36490/agri.v4i1.108.
Liu M, Chaverri P, Hodge KT. 2006. A taxonomic revision of the insect biocontrol fungus Aschersonia aleyrodis, its allies with white stromata and their Hypocrella sexual states. Mycol Res 110: 537-554. DOI: 10.1016/j.mycres.2006.01.013.
Pérez-González O, Gomez-Flores R, Tamez-Guerra P. 2022. Biocontrol of citrus blackfly, Aleurocanthus woglumi Ashby (Homoptera: Aleyrodidae), by spraying Aschersonia sp. conidia collected from infected nymphs in Quintana Roo, Mexico. Florida Entomol 105 (3): 250-254. DOI: 10.1653/024.105.0311.
Prayogo Y, Bayu MSYI, Indiati SW, Mejaya MJ. 2022. Morphological characters and efficacy of thirteen entomopathogenic fungi of Aschersonia aleyrodis webber isolates on whitefly (Bemisia tabaci Gennadius). AIP Conf Proce 2462: 060008. DOI: 10.1063/5.0076067.
Pusposendjojo N. 1985. Petunjuk Praktikum Mikroskop Elektron Skaning (SEM). LAKFIP UGM (Mimeograph), Yogyakarta. [Indonesian]
Qiu J, Song F, Qiu Y, Li X, Guan X. 2013. Optimization of the medium composition of a biphasic production system for mycelial growth and spore production of Aschersonia placenta using response surface methodology. J Inv Path 112: 108-115. DOI: 10.1016/j.jip.2012.10.010.
Rizali A, Oktaviyani, Putri SDPS, Doananda M, Linggani A. 2021. Invasion of fall armyworm Spodoptera frugiperda, a new invasive pest, alters native herbivore attack intensity and natural enemy diversity. Biodiversitas 22: 3482-3488. DOI: 10.13057/biodiv/d220847.
Sani I, Ismail SI, Abdullah S, Jalinas J, Jamian S, Saad N. 2020. A review of the biology and control of whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), with special reference to biological control using entomopathogenic fungi. Insects 11: 619. DOI: 10.3390/insects11090619.
Selangga DGW, Listihani L, Temaja IGRM, Wirya GNAS, Sudiarta IP, Yuliadhi KA. 2023. Determinants of symptom variation of Pepper yellow leaf curl Indonesia virus in bell pepper and its spread by Bemisia tabaci. Biodiversitas 24: 869-877. DOI: 10.13057/biodiv/d240224.
Sudarjat, Widiantini F, Salsabila Y, Mubarok S. 2024. The effect of conidia density and application frequency of the entomopathogenic fungus Aschersonia aleyrodis Webber in controlling silverleaf whitefly (Bemisia tabaci Gennadius) on tomato (Solanum lycopersicum Linnaeus). Plant Prot Sci 60 (1): 80-88. DOI: 10.17221/74/2023-PPS.
Sudiarta IP, Suputra IPW, Wirya GNAS. 2019. New report of insect pathogenic fungi (Aschersonia sp.) of citrus whitefly (Dialeurodes sp.) in Bali Indonesia. Agri Vet Sci 3 (1): 22-27.
Sulistyo A, Inayati A. 2016. Mechanisms of antixenosis, antibiosis, and tolerance of fourteen soybean genotypes in response to whiteflies (Bemisia tabaci). Biodiversitas 17: 447-453. DOI: 10.13057/biodiv/d170207.
Suputra IPW, Sudiarta IP, Wirya GNAS, Sumiartha IK. 2019. New report of distribution of entomopathogenic fungi Aschersonia sp. infected whitefly in Bali. Agrotrop 9 (2): 188-196. DOI: 10.24843/AJoAS.2019.v09.i02.p10.
Taufik M, Hasan A, Mallarangeng R, Gusnawaty HS, Khaeruni A, Botek M, Syair S. 2023. Relationship between whitefly (Bemisia tabaci) population and pepper yellow leaf curl disease on chili plant yield in the field. Cropsaver 6: 19-25. DOI: 10.24198/cropsaver.v6i1.44927.
Tricahyati T,Suparman, Irsan C. 2022. Natural enemies of Pentalonia nigronervosa, vector of banana bunchy top virus. Biodiversitas 23 (7): 3675-3684. DOI: 10.13057/biodiv/d230745.
Wang, Pingping, Xuhong Song, Hongyu Zhang. 2013. Isolation and characterization of Aschersonia placenta from citrus orchards and its pathogenicity towards Dialeurodes citri (Ashmead). J Invert Path 112 (2): 122-128. DOI: 10.1016/j.jip.2012.10.005.
Xie L, Han JH, Kim JJ, Lee SY. 2016. Effects of culture conditions on conidial production of the sweet potato whitefly pathogenic fungus Isaria javanica. Mycosciense 57: 64-70. DOI: 10.1016/j.myc.2015.09.002.
Yuliadhi KA, Listihani L, Selangga DGW, Sudiarta IP, Wirya GNAS, Temaja IGRM. 2024. Current status of research on Aleurodicus dugesii (Hemiptera: Aleyrodidae) on dutch eggplant. Biodiversitas 25 (5): 2241-2248. DOI: 10.13057/biodiv/d250542.
Zhang C, Ali S, Musa PD, Wang XM, Qiu BL. 2017. Evaluation of the pathogenicity of Aschersonia aleyrodis on Bemisia tabaci in the laboratory and greenhouse. Bio Sci Technol 27 (2): 210-221. DOI: 10.1080/09583157.2016.1274878.
Zhang C, Shao ZF, Han YY, Wang XM, Wang ZQ, Musa PD, Qiu BL, Ali S. 2018. Effects of Aschersonia aleyrodis on the life table and demographic parameters of Bemisia tabaci. J Integr Agric 17: 389-396. DOI: 10.1016/S2095-3119(17)61773-8.
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