Genetic variability and multi-trait selection of biomass and stalk juice for bioenergy in sorghum segregating populations

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Vol. 25 No. 11 (2024)
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DARIUS DARE
Program of Plant Breeding and Biotechnology, Graduate School, Institut Pertanian Bogor. Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, West Java, Indonesia
TRIKOESOEMANINGTYAS ♥
Department of Agronomy and Horticulture, Faculty of Agriculture, Institut Pertanian Bogor. Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, West Java, Indonesia
DESTA WIRNAS
Department of Agronomy and Horticulture, Faculty of Agriculture, Institut Pertanian Bogor. Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, West Java, Indonesia
DIDY SOEPANDI
Department of Agronomy and Horticulture, Faculty of Agriculture, Institut Pertanian Bogor. Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, West Java, Indonesia

Abstract

Abstract. Dare D, Trikoesoemaningtyas, Wirnas D, Soepandi D. 2024. Genetic variability and multi-trait selection of biomass and stalk juice for bioenergy in sorghum segregating populations. Biodiversitas 25: 4425-4437. Sorghum (Sorghum bicolor (L.) Moench) is a promising crop for biofuel production due to its high sugar content in stem juice, which can be converted into bioethanol. However, the challenge for plant breeders developing bioenergy sorghum is creating superior genotypes with enhanced biomass-related traits. This study aimed to evaluate the genetic variability and multi-trait selection of biomass and stalk juice in sorghum segregating populations. A total of 320 F2 genotypes and six check genotypes were evaluated. Observed parameters included biomass-related traits and grain yield. Data analysis comprised gene action and genetic parameter estimation, Pearson correlation, path analysis, transgressive segregant estimation, multi-trait selection, cluster, and principal component analysis. The results showed high genetic variability for biomass-related traits, with coefficients of variation for fresh biomass and juice volume of 40.64% and 68.03%, respectively. Broad-sense heritability for these traits ranged from low (0%) to high (89.27) and was influenced by additive genes and complementary epistasis. All characters showed significant correlations with target traits, except for the Brix value. Direct selection for juice volume and fresh biomass weight can be achieved through stem weight. The transgressive index revealed high values, with transgressive segregants at 5.43% and 1.94%, respectively. Multi characters selection identified the 26 best genotypes for further testing.

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References
Abu-Ellail FFB, Sadan ASA, Fares WM. 2023. Cluster analysis and genetic variability of sweet sorghum (Sorghum bicolor L. Moench) genotypes using agro-morphological and juice quality traits. Electron J Plant Breed 14 (2): 371-382. DOI: 10.37992/2023.1402.041.
Anshori MF, Purwoko BS, Dewi IS, Ardie SW, Suwarno WB, Safitri H. 2019. Selection index based on multivariate analysis for selecting doubled-haploid rice lines in lowland saline prone area. Sabrao J Breed Genet 51 (2): 161-174.
Appiah-Nkansah NB, Li J, Rooney W, Wang D. 2019. A review of sweet sorgum as a viable renewable bioenergy crop and its techno-economic analysis. Renew Energy 143 (1): 1121-1132. DOI: 10.1016/j.renene.2019.05.066.
Bandara AY, Weerasooriya DK, Gobena DD, Hopper DJ, Tesso TT, Little CR. 2020. Improving sweet sorghum for enhanced juice traits and biomass. Plant Breed 139 (1): 131-140. DOI: 10.1111/pbr.12764.
Birhan T, Kassahun B, Andrew P, Mihrete G, Aregash G. 2020. Evaluation and genetic analysis of a segregating sorgum population under moisture stress conditions. J Crop Sci Biotechnol 23: 29-38. DOI: 10.1007/s12892-019-0091-0.
BMKG. 2023. BMKG online data center. Meteorological, Climatological, and Geophysical Agency, Bogor. https://bogorkab.bps.go.id/indicator /151/80/1/jumlah-curah-hujan-menurut-bulan-dan-stasiun-pos-hujan.html
Botelho TT, Leite PSDS, Parrella RADC, Nunes JAR. 2021. Strategies for multi-trait selection of sweet sorghum progenies. Crop Breed Appl Biot 21 (4): e388221410. DOI: 10.1590/1984-70332021v21n4a59.
Carvalho G, Rooney WL. 2017. Assessment of stalk properties to predict juice yield in sorghum. BioEnergy Res 10: 657-670. DOI: 10.1007/s12155-017-9829-4.
Cazzola F, Bermejo CJ, Cointry E. 2020. Transgressive segregations in two pea F2 populations and their respective F2:3 families. Pesq Agropec Bras 55: e01623. DOI: 10.1590/S1678-3921.pab2020.v55.01623.
Chudasama CR, Gami RA, Patel RN, Patel MA. 2022. Genetic analysis using griffing's approach for forage yield and components in sorghum. Electron J Plant Breed 13 (3): 1042-1050. DOI: 10.37992/2022.1303.138.
da Silva Leite PS, Botelho TT, de Oliveira Ribeiro PC, Schaffert RE, da Costa Parrella RA, Nunes JAR. 2020. Intrapopulation recurrent selection in sweet sorghum for improving sugar yield. Ind Crops Prod 143: 111910. DOI: 10.1016/j.indcrop.2019.111910.
da Silva MJ, Pastina MM, de Souza VF, Schaffert RE, Carneiro PCS, Noda RW, de Souza Carneiro JE, Damasceno CMB, da Costa Parrella RA. 2017. Phenotypic and molecular characterization of sweet sorgum accessions for bioenergy production. PLoS One 12 (8): e0183504. DOI: 10.1371/journal.pone.0183504.
de Oliveira TC, Barelli MAA, da Silva VP, Felipin-Azevedo R, Goncalves DDL, dos Santos PRJ, Poletine JP, Galbiati C, Tardin FD. 2021. Correlations between characters and path analysis in sweet sorghum (Sorghum bicolor (L.) Moench) genotypes for juice production. Aust J Crop Sci 15 (2): 290-296. DOI: 10.21475/ajcs.21.15.02.p3083.
de Resende MDV, Alves RS. 2020. Linear, generalized, hierarchical, Bayesian and random regression mixed models in genetics/genomics in plant breeding. Funct Plant Breed J 3: 121-152. DOI: 10.35418/2526-4117/v2n2a1.
Figueiredo JMMD, Parrella RADC, Nunes JAR. 2024. Genetic parameters and selection for multiple traits in sorghum for forage purposes. Crop Breed Appl Biot 24 (2): e48122429. DOI: 10.1590/1984-70332024v24n2a22.
Gebregergs G, Mekbib F, Moral MT. 2020. Estimation of genetic variability, heritability, and genetic advance in advanced lines for grain yield and yield components of sorghum (Sorghum bicolor (L.) Moench) at Humera, Western Tigray, Ethiopia. Cogent Food Agric 6: 1764181. DOI: 10.1080/23311932.2020.1764181.
Guden B, Erdurmus C, Erdal S, Uzun B. 2021. Evaluation of sweet sorghum genotypes for bioethanol yield and related traits. Biofuels Bioprod Biorefin 15 (2): 545-562. DOI: 10.1002/bbb.2169.
Herawati R, Masdar, Alnopri. 2019. Genetic analysis of grain yield of F4 populations for developing new type of upland rice. Sabrao J Breed Genet 51 (1): 68-79.
Hernandez A. 2023. Effect of the incorporation of GWAS-selected markers in genomic selection, study model: Flavonoid pigmentation traits in sorghum. Preprints 2023: 2023041235. DOI: 10.20944/preprints202304.1235.v1.
Insan RR, Wirnas D, Trikoesoemaningtyas. 2016. Estimation of genetic parameters and selection of sorghum [Sorghum bicolor (L.) Moench] RILS F5 derived from single seed descent. Intl J Agric Res 8: 95-103. DOI: 10.31227/osf.io/c3ney.
Ishimori M, Takanashi H, Hamazaki K, Atagi Y, Kajiya-Kanegae H, Fujimoto M, Yoneda J, Tokunaga T, Tsutsumi N, Iwata H. 2020. Dissecting the genetic architecture of biofuel-related traits in a sorghum breeding population. G3-Genes Genom Genet 10 (12): 4565-4577. DOI: 10.1534/g3.120.401582.
Jambormias E, Sutjahjo SH, Mattjik AA, Wahyu Y, Wirnas D, Siregar A, Patty JR, Laisina JK, Madubun EL, Ririhena RE. 2015. Transgressive segregation analysis of multiple traits in mungbean (Vigna radiata (L.) Wilczek). Sabrao J Breed Genet 47 (2): 201-213.
Jayaramachandran M, Kumaravadivel N, Eapen S, Kandasamy G. 2010. Gene action for yield attributing characters in segregating generation (M2) of sorghum (Sorghum bicolor L.). Electron J Plant Breed 1 (4): 802-805.
Karnwal MK, Singh K. 2009. Studies on genetic variability, character association and path coefficient for seed yield and its contributing traits in soybean (Glycine max (L.) Merrill). Legume Res 32: 70-73.
Kawahigashi H, Kasuga S, Okuizumi H, Hiradate S, Yonemaru JI. 2013. Evaluation of brix and sugar content in stem juice from sorghum varieties. Grassl Sci 59 (1): 11-19. DOI: 10.1111/grs.12006.
Knight R. 1979. Quantitative Genetics, Statistics and Plant Breeding. Academy Press, Brisbane.
Koide Y, Uchiyama T, Ota Y, Sakaguchi S, Tezuka A, Nagano AJ, Ishiguro S, Takamure I, Kishima Y. 2019. Genetic basis of transgressive segregation in rice heading phenotypes. G3-Genes Genom Genet 9: 1655-1662. DOI: 10.1101/257766.
Lestari EG, Dewi IS, Yunita R. 2023. Genetic variability, heritability and selection of M2 sorghum super 2 mutant lines derived from irradiation using gamma rays. Romanian Agric Res 40: 1222-4227. DOI: 10.59665/rar4003.
Lestari R, Magandhi M, Rachmadiyanto AN, Tyas KN, Primananda E, Husaini IPA, Kobayashi M. 2024. Genetic characterization of Indonesian sorghum landraces (Sorghum bicolor (L.) Moench) for yield traits. AIMS Agric Food 9 (1): 129-147. DOI: 10.3934/agrfood.2024008.
Lestari R, Tyas KN, Rachmadiyanto AN, Magandhi M, Primananda E, Husaini IPA, Kobayashi M. 2021. Response of biomass, grain production, and sugar content of four sorghum plant varieties (Sorghum bicolor (L.) Moench) to different plant densities. Open Agric 6 (1): 761-770. DOI: 10.1515/opag-2021-0055.
Liu H, Ren L, Spiertz H, Zhu Y, Hui G. 2015. An economic analysis of sweet sorghum cultivation for ethanol production in North China. GCB Bioenergy 7 (5): 1176-1184. DOI: 10.1111/gcbb.12222.
López SI, Zavala GF, Levin L, Ruiz HA, Hernández LCE, Gutiérrez SG. 2021. Evaluation of bioethanol production from sweet sorghum variety roger under different tillage and fertilizer treatments. BioEnerg Res 14 (4): 1058-1069. DOI: 10.4067/S0718-58392021000100092.
Lorentz LH, Fortes FDO, Lúcio ADC. 2006. Análise de trilha entre as variáveis das análises de sementes de espécies florestais exóticas do Rio Grande do Sul. Rev Árvore 30 (4): 567-574. DOI: 10.1590/S0100-67622006000400009.
Luquet D, Perrier L, Clément VA, Jaffuel S, Verdeil JL, Roques S, Pot D. 2019. Genotypic covariations of traits underlying sorghum stem biomass production and quality and their regulations by water availability: Insight from studies at organ and tissue levels. GCB Bioenergy 11 (2): 444-462. DOI: 10.1111/gcbb.12571.
Maryono MY, Wirnas D, Human S. 2019. Analisis genetik dan seleksi segregan transgresif pada populasi F2 sorgum hasil persilangan B69× Numbu dan B69× Kawali. J Agron Indonesia 47 (2): 163-170. DOI: 10.24831/jai.v47i2.24991. [Indonesian]
Mathur S, Umakanth AV, Tonapi VA, Sharma R, Sharma MK. 2017. Sweet sorghum as biofuel feedstock: Recent advances and available resources. Biotechnol Biofuels 10: 146. DOI: 10.1186/s13068-017-0834-9.
Mohammed R, Are AK, Bhavanasi R, Munghate RS, Kavi Kishor PB, Sharma HC. 2015. Quantitative genetic analysis of agronomic and morphological traits in sorgum (Sorgum bicolor). Front Plant Sci 6: 945. DOI: 10.3389/fpls.2015.00945.
Munarti, Wirnas D, Trikoesoemaningtyas, Syukur M, Sobir, Sopandie D. 2022. Genetic control of stay greenness and yield and identification of transgressive segregants in sorghum F2 populations. Indonesian J Agron 50 (1): 41-48. DOI: 10.24831/jai.v50i1.39067. [Indonesian]
Mustafa M, Syukur M, Sutjahjo SH. 2019. Inheritance study for fruit characters of tomato IPBT78 x IPBT73 using joint scaling test. IOP Conf Ser Earth Environ Sci 382: 012009. DOI: 10.1088/1755-1315/382/1/012009.
Oliveira ICM, Marçal TDS, Bernardino KDC, Ribeiro PCDO, Parrella RADC, Carneiro PCS, Schaffert RE, Carneiro JEDS. 2019. Combining ability of biomass sorgum lines for agroindustrial characters and multi-trait selection of photosensitive hybrids for energy cogeneration. Crop Sci 59: 1554-156. DOI: 10.2135/cropsci2018.11.0693.
Olivoto T, Nardino M. 2021. MGIDI: Toward an effective multivariate selection in biological experiments. Bioinformatics 37 (10): 1383-1389. DOI: 10.1093/bioinformatics/btaa981.
Putri NE, Sutjahjo SH, Trikoesoemaningtyas, Nur A, Suwarno WB, Wahyu Y. 2020. Wheat transgressive segregants and their adaptation in the tropical region. Sabrao J Breed Genet 52 (4): 506-522.
Reddy PS, Reddy BVS, Rao PS. 2014. Genotype by sowing date interaction effects on sugar yield components in sweet sorghum (Sorghum bicolor L. Moench). Sabrao J Breed Genet 46 (2): 241-255.
Rick CM, Smith PG. 1953. Novel variation in tomato species hybrids. Am Nat 87 (837): 359-373. DOI: 10.1086/281796.
Rini EP, Marwiyah S, Wirnas D. 2022. Genetic analysis in an F2 population derived from Indonesian local sorghum with new superior variety. J Agron Indonesia 50: 275-282. DOI: 10.24831/jai.v50i3.44699.
Ruan R, Zhang Y, Chen P, Liu S, Fan L, Zhou N, Ding K, Peng P, Addy M, Cheng Y, Anderson Y, Wang Y, Liu Y, Lei H, Li B. 2019. Biofuels: Introduction. In: Pandey A, Larroche C, Dussap C-G, Gnansounou E, Khanal SK, Rickie S (eds). Biofuels: Alternative Feedstocks and Conversion Processes for the Production of Liquid and Gaseous Biofuels. Second Edition. Academic Press, Cambridge. DOI: 10.1016/B978-0-12-816856-1.00001-4.
Shakoor N, Nair R, Crasta O, Morris G, Feltus A, Kresovich S. 2014. A Sorghum bicolor expression atlas reveals dynamic cultivar-specific expression profiles for vegetative tissues of grain, sweet and bioenergy sorgums. BMC Plant Biol 14: 35. DOI: 10.1186/1471-2229-14-35.
Stansfield WD. 1991. Schaum’s Outline of Theory and Problems of Genetics 3rd Edition. McGraw Hill, New York.
Sulistyowati Y, Trikoesoemaningtyas, Soepandi D, Ardie SW, Nugroho S. 2016. Estimation of genetic parameters and gene actions of sorghum (Sorghum bicolor L. Moench) tolerance to low P condition. Intl J Agron Agric Res 7 (3): 38-46.
Sun R, Zhou X, Li Y, Liu Y, Liu X, Ding M, Lv X, Li F. 2024. Identifying quantitative trait locus and candidate genes for maize (Zea mays L.) plant architecture traits based on segregating populations constructed by high-generation sister lines. Plant Breed 143 (3): 305-319. DOI: 10.1111/pbr.13166.
Vitazek I, Tulik J, Klucik J. 2018. Combustible in selected biofuels. Agronomy Res 16 (2): 593-603. DOI: 10.15159/AR.18.057.
Wirnas D, Oktanti N, Rahmi HN, Andriani D, Faturrahman F, Rini EP, Marwiyah S, Trikoesoemaningtyas, Sopandie D. 2021. Genetic analysis for designing an ideotype of high-yielding sorghum based on existing lines performance. Biodiversitas 22 (12): 5286-5292. DOI: 10.13057/biodiv/d221208.
Xu YJ, Li J, Moore C, Xin ZG, Wang DH. 2018. Physico-chemical characterization of pedigreed sorghum mutant stalks for biofuel production. Indus Crop Prod 124: 806-811. DOI: 10.1016/j.indcrop.2018.08.049.
Yahaya MA, Shimelis H, Nebie B, Ojiewo CO, Rathore A, Das R. 2023. Genetic variability and population structure of African sorghum (Sorghum bicolor (L). Moench) accessions assessed through single nucleotide polymorphisms markers. Genes 14 (7): 1480. DOI: 10.3390/genes14071480.
Ziegler ACDF, Tambarussi EV. 2022. Classifying coefficients of genetic variation and heritability for Eucalyptus spp. Crop Breed Appl Biotecnol 22: e40372222. DOI: 10.1590/1984- 70332022v22n2a12.