Phytochemicals’ compound and antioxidant activities in the food of Pongo tapanuliensis from Batang Toru Forest, North Sumatra, Indonesia

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Vol. 25 No. 10 (2024)
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HERNA FEBRIANTY SIANIPAR ♥
Department of Water Resource Management, Faculty of Engineering and Water Resource Management, Universitas HKBP Nommensen. Jl. Sangnawaluh No. 4, Siopat Suhu, Pematangsiantar 21136, North Sumatera, Indonesia
http://orcid.org/0009-0009-5297-5890
WAHYU WIDORETNO
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia
http://orcid.org/0009-0006-4453-1921
LUCHMAN HAKIM
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia
http://orcid.org/0000-0002-6059-3341
REZI RAHMI AMOLIA
Yayasan Ekosistem Lestari. Jl. Bunga Sedap Malam IX No. 3, Medan 20132, North Sumatera, Indonesia
FATCHIYAH FATCHIYAH
Institute of Biosains, Universitas Brawijaya. Jl. MT. Haryono No.161, Malang 65145, East Java, Indonesia

Abstract

Abstract. Sianipar HF, Widoretno W, Hakim L, Amolia RR, Fatchiyah F. 2024. Phytochemicals’ compound and antioxidant activities in the food of Pongo tapanuliensis from Batang Toru Forest, North Sumatra, Indonesia. Biodiversitas 25: 3454-3463. Campnosperma auriculatum (Blume) Hook.fil. and Agathis borneensis Warb. are food for Pongo tapanuliensis Nurcahyo, Meijaard, Nowak, Fredriksson & Groves, 2017 found in the Batang Toru Forest, Indonesia. Studying the phytochemical properties of both plant species is important part of conservation framework for Tapanuli orangutans because information regarding their nutrient contents and benefits still needs to be investigated. This research examined the closest components, amino acids, and phytochemicals compounds that have antioxidant activity. The C. auriculatum and A. borneensis fruit were tested for proximate, amino acid, phytochemical, total flavonoid content, LC-MS/MS, and FRAP to determine antioxidant activity. The results of proximate and amino acid tests showed that C. auriculatum has higher contents of carbohydrates (74.49%), protein (7.54%), lipids (3.73%), energy from fat (33.57Kcal/100 g), total energy (361.71Kcal /100g), L-Histidine (1704.975ppm), L-Leucine (3759.28ppm), and L-Lysine (2099.038ppm) compared to A. borneensis. The phytochemical tests of both samples were positive for containing alkaloids, flavonoids, saponins, steroids, tannins, phenolics, and quinolines. The higher flavonoid content is found in C. auriculatum (94.44±2.77mg QE/g). LC-MS/MS results for both samples, C. auriculatum that is (3alpha,20R,24Z)-3-Hydroxy-21-oxoeupha-8,24-dien-26-oic acid, terpenoid group, and A. borneensis, that is Procyanidin B2, the flavonoid group. The higher antioxidant capacity was found in C. auriculatum (4.63±0.111µg/mL). The C. auriculatum has excellent potential as a natural source of nutrition and antioxidants for the Tapanuli orangutan; therefore, it is necessary to develop a strategy by cultivating these two plants and using them in pharmacognosy for P. tapanuliensis.

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