Phytochemical, antioxidant, in silico, and in vitro anti-breast cancer effects of Erigeron sumatrensis from Gayo Highlands (Indonesia) against MCF-7 cells

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Vol. 25 No. 12 (2024)
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VIVIVERA RUSELLI PUSPA
Department of Biology Education, Faculty of Teacher Training and Education, Universitas Syiah Kuala. Jl. Teungku Hasan Krueng Kalee, Banda Aceh 23111, Aceh, Indonesia
MUHAMMAD ANSARI ADISTA
Department of Medicine, Faculty of Medicine, Universitas Syiah Kuala. Jl. Teungku Tanah Abee, Banda Aceh 23111, Aceh, Indonesia
DJUFRI ♥
Department of Biology Education, Faculty of Teacher Training and Education, Universitas Syiah Kuala. Jl. Teungku Hasan Krueng Kalee, Banda Aceh 23111, Aceh, Indonesia

Abstract

Abstract. Puspa VR, Adista MA, Djufri. 2024. Phytochemical, antioxidant, in silico, and in vitro anti-breast cancer effects of Erigeron sumatrensis from Gayo Highlands (Indonesia) against MCF-7 cells. Biodiversitas 25: 5123-5137. This study investigated the therapeutic potential of Erigeron sumatrensis, a wild herb from the Gayo Highlands, by evaluating its methanol stem extract's phytochemical profile, antioxidant activity, biological activity, molecular docking, ADMET prediction, and MTT assay using MCF-7 cells. Phytochemical analysis revealed the presence of various secondary metabolites, including flavonoids, phenolics, terpenoids, steroids, tannins, and alkaloids. The stem extract exhibited the highest total phenolic content at 959.091 mgQE/g. Antioxidant activity, assessed using the DPPH assay, was strong with an IC50 value of 96 ?g/mL. Gas chromatography-mass spectrometry analysis of the methanol extract of E. sumatrensis stems led to the identification of 24 compounds. Molecular docking studies identified three compounds-Stigmast-7-en-3-ol, (3?,5?,24S)-, Caryophyllene oxide and 1H-Cycloprop[e]azulen-7-ol, decahydro- 1,1,7-trimethyl-4-methylene-, [1ar(1a?,4a?,7?,7a?,7b?)] exhibiting potential anti-proliferative activity against MCF-7 cells. ADMET analysis predicted compliance with all identified compounds, suggesting their suitability as oral drug candidates. The in vitro cytotoxicity assay against MCF-7 cells yielded a moderate cytotoxic classification with an IC50 of 192.90 ?g/mL. These findings highlight the promising potential of E. sumatrensis as a source of bioactive compounds for developing novel anti-breast cancer drugs. However, further research is needed to explore its full therapeutic potential and facilitate its development into pharmaceutical products.

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