Investigation of endophytic bacteria associated with Paederia foetida: Population, characterization, antibacterial, and pathogenicity analysis
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Yunita M, Ohiwal M, Warella JC, Azizah SN, Asmin E, Siahaya PG. 2025. Investigation of endophytic bacteria associated with Paederia foetida: Population, characterization, antibacterial, and pathogenicity analysis 26: 981-991. The aim of this study was to obtain non-pathogenic endophytic bacterial isolates that can be developed as antibacterials for further applications. Endophyte isolation was carried out in 4 different media to obtain diverse endophytes and characterized macroscopically and microscopically. Endophyte screening was done through antibacterial tests against 10 different pathogenic bacteria. Pathogenicity analysis was performed on Blood Agar Plate (BAP) to differentiate pathogenic and non-pathogenic group of endophytic bacteria. The results showed that Starch Casein Agar was the best media for isolation in terms of total population and morphological characteristics compared to the other three media. A total of 27 isolates were collected according to the different morphology. Screening through antibacterial tests revealed that 19 endophytic isolates were significantly able to inhibit various species of pathogenic bacteria (p-value of 0.001). Pathogenicity analysis showed that 8 of 19 isolates (HR1, HR2, HR3, K2, NL3, S2, A2, and A9) were categorized as ? hemolysis, while the other 11 isolates were categorized as ? hemolysis and ? hemolysis. The study concluded that 8 endophytic bacterial isolates associated with P. foetida leaves had the strong potential to be developed and can be applied in advanced antibacterial applications.
##plugins.themes.bootstrap3.article.details##
References
Afzal I, Shinwari ZK, Sikandar, S, Shahzad, S. 2019. Plant beneficial endophytic bacteria: Mechanisms, diversity, host range and genetic determinants. Microbiol Res 221: 36-49. DOI: 10.1016/j.micres.2019.02.001.
Ali MA, Ahmed T, Ibrahim E, Rizwan M, Chong KP, Yong JWH. 2024. A review on mechanisms and prospects of endophytic bacteria in biocontrol of plant pathogenic fungi and their plant growth-promoting activities. Heliyon 10: e31573. DOI: 10.1016/j.heliyon.2024.e31573.
Anitha KG. 2019. Antioxidant producing endophytic bacterial consortium as biological tool for enhancing the antioxidant activity of bhendi under salt stress. J Pharm Phytochem 8 (5): 934-937.
Anjum N, Chandra R. 2015. Endophytic bacteria: Optimizaton of isolation procedure from various medicinal plants and their preliminary characterization. Asian J Pharm Clin Res 8 (4): 233-238.
Aswani R, Jishma P, Radhakrishnan EK. 2020. Endophytic bacteria from the medicinal plants and their potential applications. In: Ajay Kumar, Vipin Kumar Singh (eds). Food Science, Technology and Nutrition. Woodhead Publishing, Cambridge. DOI: 10.1016/B978-0-12-818734-0.00002-4.
Beiranvand M, Amin M, Hashemi-Shahraki A, Romani B, Yaghoubi S, Sadeghi P. 2017. Antimicrobial activity of endophytic bacterial populations isolated from medical plants of Iran. Iran J Microbiol 9 (1): 11-18.
Brader G, Compant S, Mitter B, Trognitz F, Sessitsch A. 2014. Metabolic potential of endophytic bacteria. Curr Opinion Biotechnol 27 (1): 30-37. DOI: 10.1016/j.copbio.2013.09.012.
Cardoso VM, Campos FF, Santos ARO, Ottoni MHF, Rosa CA, Almeida VG, Grael CFF. 2020. Biotechnological applications of the medicinal plant Pseudobrickellia brasiliensis and its isolated endophytic bacteria. J Appl Microbiol 129: 926-934. DOI: 10.1111/jam.14666.
Chanda S, Sarethy IP, De B, Singh K. 2013. Paederia foetida - a promising ethno-medicinal tribal plant of northeastern India. J For Res 24 (4): 801-808. DOI: 10.1007/s11676-013-0369-2.
dos Santos ML, Berlitz DL, Wiest SLF, Schünemann R, Knaak N, Fiuza, LM. 2018. Benefits associated with the interaction of endophytic bacteria and plants. Braz Arch Biol Technol 61 (1): e18160431. DOI: 10.1590/1678-4324-2018160431.
Duhan P, Bansal P, Rani S. 2020. Isolation, identification and characterization of endophytic bacteria from medicinal plant Tinospora cordifolia. S Afr J Bot 134: 43-49. DOI: 10.1016/j.sajb.2020.01.047.
Dutta PP, Marbaniang K, Sen S, Dey BK, Talukdar NC. 2023. A review on phytochemistry of Paederia foetida Linn. Phytomed Plus 3 (1): 100411. DOI: 10.1016/j.phyplu.2023.100411.
Eevers N, Gielen M, Sánchez-López A, Jaspers S, White JC, Vangronsveld J, Weyens N. 2015. Optimization of isolation and cultivation of bacterial endophytes through addition of plant extract to nutrient media. Microb Biotechnol 8 (4): 707-715. DOI: 10.1111/1751-7915.12291.
El-Shatoury S, Abdulla H, El-Karaaly O, El-Kazzaz W, Dewedar A. 2006. Bioactivities of endophytic actinomycetes from selected medicinal plants in the world heritage site of Saint Katherine, Egypt. Intl J Bot 2 (3): 307-312. DOI: 10.3923/ijb.2006.307.312.
Fatimah, Asritafriha L, Salsabila S, Dewi EK, Geraldi A, Ramadhan R, Suwito H, Rahman A, Riyadi L. 2024. Potential of antimicrobial-producing endophytic bacteria from Balikpapan endemic ginger (Etlingera balikpapanensis A.D. Poulsen). Biodiversitas 25 (9): 3005-3013. DOI: 10.13057/biodiv/d250922.
Hikmawati F, Susilowati A, Setyaningsih R. 2019. Deteksi jumlah dan uji patogenitas Vibrio spp. pada kerang hijau (Perna viridis) di kawasan wisata Pantai Yogyakarta. J Pros Sem Nas Masy Biodiv Indo 5 (2): 334-339. [Indonesian]
Ira P, Sujatha S, Chandra PS. 2013. Virulence factors in clinical and commensal isolates of Enterococcus species. Indones J Pathol Microbiol 56 (1): 24-30. DOI: 0.4103/0377-4929.116144.
Kandasamy GD, Kathirvel P. 2023. Insights into bacterial endophytic diversity and isolation with a focus on their potential applications-A review. Microbiol Res 266: 127256. DOI: 10.1016/j.micres.2022.127256.
Khare E, Mishra J, Arora NK. 2018. Multifaceted interactions between endophytes and plant: Developments and prospects. Front Microbiol 9: 2732. DOI: 10.3389/fmicb.2018.02732.
Kumar V, Al-Abbasi FA, Ahmed D, Verma A, Mujeeb M, Anwar F. 2015. Paederia foetida Linn. inhibits adjuvant induced arthritis by suppression of PGE2 and COX-2 expression via nuclear factor-?B. Food Funct 6 (5): 1652-1666. DOI: 10.1039/c5fo00178a.
Kumar V, Anwar F, Ahmed D, Verma A, Ahmed A, Damanhouri ZA, Mishra V, Ramteke PW, Bhatt PC, Mujeeb M. 2014. Paederia foetida Linn. leaf extract: An antihyperlipidemic, antihyperglycaemic and antioxidant activity. BMC Compl Alter Med 14: 76. DOI: 10.1186/1472-6882-14-76.
Liu YH, Guo JW, Salam N, Li L, Zhang YG, Han J, Mohamad OA, Li WJ. 2016. Culturable endophytic bacteria associated with medicinal plant Ferula songorica: Molecular phylogeny, distribution and screening for industrially important traits. 3 Biotech 6 (2): 209. DOI: 10.1007/s13205-016-0522-7.
Maela MP, van der Walt H, Serepa-Dlamini MH. 2022. The antibacterial, antitumor activities, and bioactive constituents’ identification of Alectra sessiliflora bacterial endophytes. Front Microbiol 1 (1): 870821. DOI: 10.3389/fmicb.2022.870821.
Morales-Cedeño LR, Orozco-Mosqueda M del C, Loeza-Lara PD, Parra-Cota F, de los Santos-Villalobos S, Santoyo G. 2021. Plant growth-promoting bacterial endophytes as biocontrol agents of pre- and post-harvest diseases: Fundamentals, methods of application and future perspectives. Microbiol Res 242: 126612. DOI: 10.1016/j.micres.2020.126612.
Moretti C, Rezzonico F, Orfei B, Cortese C, Moreno?Pérez A. van den, Burg HA, Buonaurio R. 2021. Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP?PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP?PG 722. Mol Plant Pathol 22 (10): 1209-1225. DOI: 10.1111/mpp.13105.
Mugiastuti E, Manan A, Soesanto L. 2023. Biological control of maize downy mildew with the antagonistic bacterial consortium. Biodiversitas 24 (9): 4644-4650. DOI: 10.13057/biodiv/d240904.
Newman DJ. 2018. Are microbial endophytes the actual producers of bioactive antitumor agents?. Trends Cancer 4 (10): 662-670. DOI: 10.1016/j.trecan.2018.08.002.
Pal A, Chattopadhyay A, Paul AK. 2012. Diversity and antimicrobial spectrum of endophytic bacteria isolates from Peaderia foetida L. Intl J Curr Pharm Res 4 (3): 32.
Parul B, Sharma B, Jain U. 2014. Virulence associated factors and antibiotic sensitivity pattern of Escherichia coli isolated from cattle and soil. Vet World 7: 369-372. DOI: 10.14202/vetworld.2014.369-372.
Passari AK, Leo VV, Chandra P, Kumar B, Nayak C, Hashem A, Abd Allah EF, Alqarawi AA, Singh BP. 2018. Bioprospection of actinobacteria derived from freshwater sediments for their potential to produce antimicrobial compounds. Microb Cell Fact 17 (1): 68. DOI: 10.1186/s12934-018-0912-0.
Rajesh PS, Ravishankar RV. 2014. Quorum quenching activity in cell-free lysate of endophytic bacteria isolated from Pterocarpus santalinus Linn., and its effect on quorum sensing regulated biofilm in Pseudomonas aeruginosa PAO1. Microbiol Res 169 (7-8): 561-569. DOI: 10.1016/j.micres.2013.10.005.
Russell FM, Biribo SSN, Selvaraj G, Oppedisano F, Warren S, Seduadua A, Mulholland EK, Carapetis JR. 2006. As a bacterial culture medium, citrated sheep blood agar is a practical alternative to citrated human blood agar in laboratories of developing countries. J Clin Microbiol 44 (9): 3346-3351. DOI: 10.1128/JCM.02631-05.
Rustamova N, Wubulikasimu A, Mukhamedov N, Gao Y, Egamberdieva D, Yili A. 2020. Endophytic bacteria associated with medicinal plant Vernonia anthelmintica: Diversity and characterization. Curr Microbiol 77 (8): 1457-1465. DOI: 10.1007/s00284-020-01924-5.
Said A, El-Gamal MS, Abu-Elghait M, Salem SS. 2021. Isolation, Identification and antibiotic susceptibility pattern of urinary tract infection bacterial isolates. Lett Appl NanoBioSci 10 (4): 2820-2830. DOI: 10.33263/LIANBS104.28202830.
Savitri L, Tiyas EW, Krissanjaya R. 2024. Testing antibacterial activity of daun kentut (Paederia foetida L.) leaf extract against Escherichia coli in vitro. Biol Med Nat Prod Chem 13 (1): 277-283. DOI: 10.14421/biomedich.2024.131.277-283.
Singh AK, Sharma RK, Sharma V, Singh T, Kumar R, Kumari D. 2017. Isolation, morphological identification and in vitro antibacterial activity of endophytic bacteria isolated from Azadirachta indica (neem) leaves. Vet World 10: 510-516. DOI: 10.14202/vetworld.2017.510-516.
Singh R, Bharose R, Singh VK. 2018. Effect of Modern agricultural practices on biological properties of rhizospheric and non rhizospheric soils of chaka block of Allahabad District, U.P. Intl J Curr Microbiol Appl Sci 7 (12): 1496-1504. DOI: 10.20546/ijcmas.2018.712.176.
Singh R, Pandey KD, Singh M, Singh SK, Hashem A, Al?arjani ABF, Abd_allah EF, Singh PK, Kumar A. 2022. Isolation and characterization of endophytes bacterial strains of Momordica charantia L. and their possible approach in stress management. Microorganisms 10 (2): 290. DOI: 10.3390/microorganisms10020290.
Sulistiyani S, Ardyati T, Winarsih S. 2016. Antimicrobial and antioxidant activity of endophyte bacteria associated with Curcuma longa rhizome. J Exp Life Sci 6: 45-51. DOI: 10.21776/ub.jels.2016.006.01.11.
Sushma M, Jayasangkar M, Vinu AK. 2018. Antibacterial activity of endophytic bacteria isolated from few medicinal plants of BR hills, Karnataka. J Pharm Phytochem 7 (5): 2338-2342.
Triandriani W, Sogandi, Saputri DD, Suhendar U. 2020. Antioxidant activity of endophytic bacterial extract isolated from clove leaf (Syzygium aromaticum L.). J Agri Appl Biol 1 (1): 9-17. DOI: 10.11594/jaab.01.01.02.
Vinodkumar S, Nakkeeran S, Renukadevi P, Mohankumar S. 2018. Diversity and antiviral potential of rhizospheric and endophytic Bacillus species and phyto-antiviral principles against tobacco streak virus in cotton. Agric Ecosyst Environ 267: 42-51. DOI: 0.1016/j.agee.2018.08.008.
Widjajanti H, Handayani CV, Nurnawati E. 2021. Antibacterial activity of endophytic fungi from Sembukan (Paederia foetida L.) leaves. Sci Technol Indonesia 6: 189-195. DOI: 10.26554/sti.2021.6.3.189-195.
Wo?niak M, Ty?kiewicz R, Siebielec S, Ga??zka A, Jaroszuk-?cise? J. 2023. Metabolic profiling of endophytic bacteria in relation to their potential application as components of multi-task biopreparations. Microbiol Ecol 86: 2527-2540. DOI: 10.1007/s00248-023-02260-4.
Wu W, Chen W, Liu S, Wu J, Zhu Y, Qin L, Zhu B. 2021. Beneficial relationships between endophytic bacteria and medicinal plants. Front Plant Sci 12 (1): 646146. DOI: 10.3389/fpls.2021.646146.
Yunita M, Mubarik NR, Solihin DD. 2016. Isolation and identification of chitinolytic bacteria as biocontrol agent of pathogenic fungi on gold silkworm cocoon Cricula trifenestrata. Malay J Microbiol 12(1): 69-75. DOI: 10.21161/mjm.74415.
Yunita M, Ohiwal M, Dirks CS, Angkejaya OW, Sukmawati S, Ilsan NA. 2022a. Endophytic bacteria associated with Myristica fragrans: Improved media, bacterial population, preliminary characterization, and potential as antibacterials. Biodiversitas 23 (8): 4047-4054. DOI: 10.13057/biodiv/d230824.
Yunita M, Manse Y, Wulandari MC, Sukmawati S, Ohiwal M. 2022b. Isolation of endophytic bacteria from nutmeg plant as antibacterial agents against pathogenic bacteria. Jurnal Pendidikan Biologi 7 (1): 115-122. DOI: 10.31932/jpbio.v7i1.1522.
Yunita M, Ohiwal M, Elfitrasya MZ, Rahawarin H. 2023a. Antibacterial activity of Paederia foetida leaves using two different extraction procedures against pathogenic bacteria. Biodiversitas 24 (11): 5920-5927. DOI: 10.13057/biodiv/d241110.
Yunita M, Lumbantobing RM, Tahitu R. 2023b. Antifungal activity of endophytic bacteria isolated from miana plants (Coleus scutellarioides (L.) Benth.) against Candida albicans. Folia Med Indonesia 59 (3): 274-281. DOI: 10.20473/fmi.v59i3.43989.