Identification of rhizobacteria isolate from Bali Barat National Park, Indonesia and the potential as biological agents against soybean seed-borne pathogen
Article Sidebar
Abstract Views: 321
File Views: 57
Main Article Content
Abstract
Abstract. Khalimi K, Pranatayana IBG, Yuliadhi KA, Gargita IWD, Yudha IKW. 2025. Identification of rhizobacteria isolate from Bali Barat National Park, Indonesia and the potential as biological agents against soybean seed-borne pathogen. Biodiversitas 26: 1799-1806. The use of biological agents is a method of suppressing plant pathogenic fungi. Therefore, this study aimed to determine biochemical characteristics, identity, antifungal activity, and types of compounds produced by rhizobacteria isolates from Taman National Bali Barat (West Bali National Park/TNBB), Bali, Indonesia. Rhizobacteria was characterized using Microbact Biochemical Kits and molecular identification of rhizobacteria based on the 16S rRNA gene sequence analysis. Antifungal compounds were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). The results showed that rhizobacteria used gelatin and arabinose as carbon sources, producing indole, acetoin, urease, and tryptophan deaminase compounds. The identity of rhizobacteria isolate TNBB is Bacillus thuringiensis. Furthermore, rhizobacteria filtrate of TNBB isolate inhibited the growth of Alternaria alternata, Aspergillus flavus, Aspergillus parasiticus, Aspergillus ochraceus, Aspergillus niger, Aspergillus tubingensis, Aspergillus aculeatus, and Rhizopus oryzae fungi. The diameter of the inhibition zone formed was categorized as having very strong power. The filtrate of TNBB isolate rhizobacteria contained 16 antifungal compounds, namely 1,3,5-triazine-2,4,6-triamine, 2(1H)-pyridinone 6-hydroxy-, tridecanoic acid, N-acetyl-d-glucosamine, hexadecanoic acid, butanoic acid, pentyl ester, pentanoic acid butyl ester, pentanoic acid pentyl ester, linoleic acid, 1-naphthalene-sulfonic acid, 2-naphthalene-sulfonic acid, stearic acid, cyclotrisiloxane hexamethyl-, cyclotrisiloxane hexamethyl, 1,2-benzenedicarboxylic acid, diisooctyl ester, and benzo[h]quinoline, 2,4-dimethyl-. The results of this study provided information that TNBB isolate rhizobacteria are suitable for use as biological agents.
Article Details
Issue
Section
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
References
Amza J. 2018. Seed borne fungi, food spoilage, the negative impact and their management: A review. Food Sci Quality Manag 81: 70-79.
Antoci V, Oniciuc L, Mantu DA, Moldoveanu C, Mangalagiu V, Amarandei AM, Lungu CN, Dunca S, Mangalagiu II, Zbancioc G. 2021. Benzoquinoline derivatives: A straightforward and efficient route to antibacterial and antifungal agents. Pharmaceuticals 14 (335): 1-21. DOI: 10.3390/ph14040335.
Bonnet M, Lagier JC, Raoult D, Khelaifia S. 2019. Bacterial culture through selective and non-selective conditions: The evolution of culture media in clinical microbiology. New Microbe New Infect 34: 1-11. DOI: 10.1016/j.nmni.2019.100622.
Chadotra SN, Baldaniya BB. 2019. Synthesis and characterizations of some new 1,3,5-triazine-2,4,6-Triamine based derivatives as potent antimicrobial and antiinfective agents. Intl J Sci Res Chem 4 (1): 29-38.
Choub V, Won SJ, Ajuna HB, Moon JH, Choi SI, Lim HI, Ahn YS. 2022. Antifungal activity of volatile organic compounds from Bacillus velezensis CE100 against Colletotrichum gloeosporioides. Horticulturae 8 (557): 2-13. DOI: 10.3390/horticulturae8060557.
Garbeva P, Weisskop L. 2020. Airborne medicine: Bacterial volatiles and their in?uence on plant health. New Phytol 226: 32-43. DOI: 10.1111/nph.16282.
Guimaraes A, Venancio A. 2022. The potential of fatty acids and their derivatives as antifungal agents: A review. Toxins 14 (188): 1-21. DOI: 10.3390/toxins14030188.
Hashem AH, Saied E, Amin BH, Alotibi FO, Askar AA, Arishi A, Elkady FM, Elbahnasawy MA. 2022. Antifungal activity of biosynthesized silver nanoparticles (AgNPs) against Aspergilli causing Aspergillosis: Ultrastructure study. J Funct Biomater 13 (242): 2-17. DOI: 10.3390/jfb13040242.
Hawar SN, Taha ZK, Hamied AS, Shmgani HS, Sulaiman GM, Elsilk S. 2023. Antifungal activity of bioactive compounds produced by the endophytic fungus Paecilomyces sp. (JN227071.1) against Rhizoctonia solani. Intl J Biomaterials 2023 (1): 2411555. DOI: 10.1155/2023/2411555.
He CN, Ye WQ, Zhu YY, Zhou WW. 2020. Antifungal activity of volatile organic compounds produced by Bacillus methylotrophicus and Bacillus thuringiensis against five common spoilage fungi on loquats. Molecules 25 (3360): 1-14. DOI: 10.3390/molecules25153360.
Kadjo AC, Beugre GC, Tchotch DAS, Kedjebo KBD, Mounjouenpou P, Durand N, Fontana A, Guehi ST. 2023. Screening of anti-fungal bacillus strains and influence of their application on cocoa beans fermentation and final bean quality. J Adv Micro 23 (1): 8-17. DOI: 10.9734/JAMB/2023/v23i1700.
Kazerooni EA, Maharachchikumbura SSN, Sadi AM, Kang SM, Yun BW, Lee IJ. 2021. Biocontrol potential of Bacillus amyloliquefaciens against Botrytis pelargonii and Alternaria alternata on Capsicum annuum. J Fungi 7 (472): 2-27. DOI: 10.3390/jof7060472.
Kryszczuk IP, Pankiewicz U, Paszt LS. 2023. Biological control of Aspergillus parasiticus and Aspergillus ochraceus and reductions in the amount of ochratoxin a and aflatoxins in bread by selected non-conventional yeast. Food 12 (3871): 1-14. DOI: 10.3390/foods12203871.
Lee JH, Kim YG, Khadke SK, Lee J. 2020. Antibiofilm and antifungal activities of medium-chain fatty acids against Candida albicans via mimicking of the quorum-sensing molecule farnesol. Microb Biotech 14 (4): 1353-1366. DOI: 10.1111/1751-7915.13710.
Lin S, Liu C, Zhao X, Han X, Li X, Ye Y, Li Z. 2022. Recent advances of pyridinone in medicinal chemistry. Front Chem 10: 869860. DOI: 10.3389/fchem.2022.869860.
Matniyazova H, Tillaboyeva D, Ergasheva G, Shaxmurova G, Yuldashov U, Sherimbetov. 2024. Alternaria alternata fungus effects on physiological and biochemical processes of soybean. SABRAO J Breed Genet 56 (3): 1060-1071. DOI: 10.54910/sabrao2024.56.3.14.
Mena L, Billamboz M, Charlet R, Despres B, Sendid B, Ghinet A, Jawhara S. 2022. Two new compounds containing pyridinone or triazine heterocycles have antifungal properties against Candida albicans. Antibiotics 11 (72): 2-16. DOI: 10.3390/antibiotics11010072.
Minervini F, Missaoui J, Celano G, Calasso M, Achour L, Saidane D, Gobbetti M, Angelis MD. 2019. Use of autochthonous Lactobacilli to increase the safety of Zgougou. Microorganisms 8 (29): 2-20. DOI: 10.3390/microorganisms8010029.
Nnamani GC, Gana AS, Shaahu A, Msaakpa TS, Lemibe PC, Nnamani MA, Nwanyanwu EO. 2021. Effects of aflatoxin on soybean. J Plant Develop 28: 161-168. DOI: 10.47743/jpd.2021.28.1.855.
Patel JK, Mistry Y, Soni R, Jha A. 2024. Evaluation of antifungal activity of endophytic Bacillus spp. and identification of secondary metabolites produced against the phytopathogenic fungi. Curr Microbiol 81 (5): 128. DOI: 10.1007/s00284-024-03652-6.
Paudel B, Bhattarai HD, Kim IC, Lee H, Sofronov R, Ivanova L, Yim JH. 2014. Estimation of antioxidant, antimicrobial activity and brine shrimp toxicity of plants collected from Oymyakon region of the Republic of Sakha (Yakutia), Russia. Biol Res 47 (1): 1-6. DOI: 10.1186/0717-6287-47-10.
Pippi B, Paula R, Gabriella RMM, Vanessa ZB, Daiane FDL, Mario LT, Lucas LF, Ricardo JA, Saulo FA, Alexandre MF. 2017. Evaluation of 8-Hydroxyquinoline derivatives as hits for antifungal drug design. Med Mycol 55 (7): 763-773. DOI: 10.1093/mmy/myx003.
Prasath KG, Tharani H, Mourya SK, Shunmugiah KP. 2020. Palmitic acid inhibits the virulence factors of candida tropicalis: Biofilms, cell surface hydrophobicity, ergosterol biosynthesis, and enzymatic activity. Front Microbiol 11 (864): 1-21. DOI: 10.3389/fmicb.2020.00864.
Ravn JL, Martens HJ, Pettersson D, Pedersen NR. 2015. Enzymatic solubilisation and degradation of soybean fibre demonstrated by viscosity, fibre analysis and microscopy. Intl J Biol Sci 7 (9): 1-13. DOI: 10.5539/jas.v7n9p1.
Saleh AE, Hassan ZU, Zeidan R, Shamary N, Yafei T, Alnaimi H, Higazy NS, Migheli Q, Jaoua S. 2021. Biocontrol activity of Bacillus megaterium BM344-1. ACS Omega 6: 10984-10990. DOI: 10.1021/acsomega.1c00816.
Saleh LY, Altintas B, Filiciotto L, Zorlu Y, Luque R, Ulger M, Dondas HA, Altug C. 2022. Structural assessment of novel spiro-naphthalene-1.2'- [1,3,4] oxadiazol-4-ones prepared under batch and flow chemistry with a concise antifungal and anti(myco) bacterial activity. Tetrahedron 131: 1-9. DOI: 10.1016/j.tet.2022.133231.
Santoso I, Fadhilah QG, Maryanto AE, Yasman. 2020. Antagonist effect of Bacillus spp. against Aspergillus niger CP isolated from cocopeat powder. IOP Conf Ser Earth Environ Sci 846 (1): p.012001. DOI: 10.1088/1755-1315/846/1/012001.
Seyfarth F, Schliemann S, Elsner P, Hipler UC. 2008. Antifungal effect of high- and low-molecular-weight chitosan hydrochloride, carboxymethyl chitosan, chitosan oligosaccharide and N-acetyl-d-glucosamine against Candida albicans, Candida krusei and Candida glabrata. Intl J Pharm 353: 139-148. DOI: 10.1016/j.ijpharm.2007.11.029.
Soesanto L, Hartono ARR, Mugiastuti E, Widarta H. 2020. Seed-borne pathogenic fungi on some soybean varieties. Biodiversitas 21 (9): 410-415. DOI: 10.13057/biodiv/d210911.
Souza TD, Massarolo KC, Tralamazza SM, Furlong EB. 2018. Monitoring of fungal biomass changed by Rhizopus oryzae in relation to amino acid and essential fatty acids profile in soybean meal, wheat and rice. CyTA - J Food 16 (1): 156-164. DOI: 10.1080/19476337.2017.1359676.
Xie F, Hao Y, Liu J, Bao J, Ni T, Liu Y, Chi X, Wang T, Yu S, Jin Y, Li L, Zhang D, Yan L. 2022. Discovery of novel thiosemicarbazides containing 1,3,5-Triazines derivatives as potential synergists against fluconazole-resistant Candida albicans. Pharmaceutics 14 (2334): 1-16. DOI: 10.3390/pharmaceutics14112334.
Yang MQ, Wang ZJ, Zhai CB, Chen LQ. 2024. Research progress on the application of 16S rRNA gene sequencing and machine learning in forensic microbiome individual identification. Front Microbiol 15: 1360457. DOI: 10.3389/fmicb.2024.1360457.
Yao H, Liu S, Chang R, Liu T, Ma D, Han X, Lin C, Zhou Z, Mao J. 2024. Purification, characterisation and visualisation of soybean protein hydrolysis by aspergillopepsin I from mangrove Aspergillus tubingensis. Intl Biodeterior Biodegrad 190: 105798. DOI: 10.1016/j.ibiod.2024.105798.
Yuan S, Wu Y, Jin J, Tong S, Zhang L, Cai Y. 2023. Biocontrol capabilities of Bacillus subtilis E11 against Aspergillus flavus in vitro and for dried red chili (Capsicum annuum L.). Toxins 15 (308): 2-15. DOI: 10.3390/toxins15050308.
Zeatar AR, Enaina IMMA, Zayedb A, Mahmoudc AG, Elkhawaga MA. 2022. Screening and identification of actinomycetes from animals’ dung for antimicrobial activity. Delta J Sci 44 (2): 35-56. DOI: 10.21608/djs.2022.135387.1022.
Zhu X, Hua Y, Li X, Kong X, Zhang C, Chen Y. 2021. Isolation and characterization of an activator-dependent protease from Aspergillus ochraceus screened from low denatured defatted soybean meal and the proteolysis of soy proteins. LWT-Food Sci Tech 150: 112026. DOI: 10.1016/j.lwt.2021.112026.