Symbiotic potential of endophytic fungi from native orchids with histological insights and growth responses in Dendrobium hybrid
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Abstract. Sukamto DS, Avivi S, Nurcahyanti SD, Restanto DP. 2025. Symbiotic potential of endophytic fungi from native orchids with histological insights and growth responses in Dendrobium hybrid. Biodiversitas 26: 6389-6398. Endophytic fungi play an essential role in orchid development by facilitating nutrient exchange and supporting early growth through peloton formation in root tissues. This study evaluated the symbiotic potential of eight fungal isolates obtained from native orchids in Jember, East Java, Indonesia, and examined their effects on peloton induction, root colonization, and seedling growth of a Dendrobium hybrid. Each isolate was identified morphologically and tested through inoculation assays, histological analyses, and seedling performance measurements. Four isolates—Ceratobasidium (1), Rhizoctonia sp. (3 and 4), and Tulasnella (6)—successfully colonized the root cortex and produced pelotons, whereas isolates 2 (Fusarium) and 8 (Cladosporium) caused pathogenic symptoms, including root and leaf rot, and were excluded from growth evaluation. Growth assays showed that Rhizoctonia isolate 4 produced the strongest symbiotic response, increasing shoot number by 175% and shoot length by more than tenfold relative to controls, followed by moderate enhancements from isolates 1, 5, 6, and 7. These findings show that native orchid-associated endophytes possess strong mycorrhizal potential and can be developed as effective bioinoculants to enhance the early growth of Dendrobium hybrids. This study provides a foundational step toward utilizing locally sourced symbiotic fungi to support orchid propagation and conservation efforts.
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References
Al-Zaidi AKN, Al-Mharib MZK. 2025. Response of growth and yield of potato plants to the addition of biofertilizers, Nile flower peat fertilizer, and spraying with its extract. Iraqi J Agric Sci 56: 456-468. DOI: 10.36103/h499ej86.
Aljuboori FK, Ibrahim BY, Mohamed AH. 2022. Biological control of the complex disease of Rhizoctonia solani and root-knot nematode Meloidogyne javanica on chickpea by Glomus spp. and Pseudomonas sp. Iraqi J Agric Sci 53: 669-676. DOI: 10.36103/ijas.v53i3.1577.
Arobaya AYS. 2022. Variasi morfologi bunga anggrek bulan hybrida Phalaenopsis amabilis: Analisa karakter dengan pendekatan numerik. Biota Jurnal Ilmiah Ilmu-Ilmu Hayati 7: 70-85. DOI: 10.24002/biota.v7i1.4207. [Indonesian]
Bell J, Yokoya K, Kendon JP, Sarasan V. 2020. Diversity of root-associated culturable fungi of Cephalanthera rubra in relation to soil characteristics. PeerJ 8: e8695. DOI: 10.7717/peerj.8695.
Bhuiyan MS, Hossain MM, Hossain KS, Islam MN. 2021. Isolation and identification of mycorrhizal fungus from an epiphytic orchid (Rhynchostylis retusa). Bangladesh J Bot 50: 85-91. DOI: 10.3329/bjb.v50i1.52675.
Damanik AJ, Masitoh S, Prayogo H. 2018. Studi keanekaragaman jenis anggrek (Orchidaceae) berdasarkan ketinggian tempat di Bukit Wangkang Kabupaten Kubu Raya. Jurnal Hutan Lestari 6: 447-455. DOI: 10.26418/jhl.v10i4. [Indonesian]
Dearnaley J, Perotto S, Selosse MA. 2017. Structure and development of orchid mycorrhizas. In: Molecular Mycorrhizal Symbiosis. John Wiley & Sons, Inc., New Jersey. DOI: 10.1002/9781118951446.ch5.
Decruse SW, Neethu RS, Pradeep NS. 2018. Seed germination and seedling growth promoted by a Ceratobasidiaceae clone in Vanda thwaitesii, an endangered orchid species. S Afr J Bot 116: 222-229. DOI: 10.1016/j.sajb.2018.04.002.
El-Dawy EGAEM, Gherbawy YA, Hussein MA. 2021. Morphological, molecular characterization, plant pathogenicity, and biocontrol of Cladosporium complex groups associated with faba beans. Sci Rep 11: 1-12. DOI: 10.1038/s41598-021-93123-w.
Eldina G, Wirawan IGPS, Sritamin M. 2021. Microscopic identification of vesicular arbuscular mycorrhizae associated with Cymbidium sp. Intl J Biosci Biotechnol 9: 15-23. DOI: 10.24843/IJBB.2021.v09.i01.p02.
Fujimori S, Abe JP, Okane I, Yamaoka Y. 2019. Three new species in the genus Tulasnella isolated from orchid mycorrhiza of Spiranthes sinensis var. amoena. Mycoscience 60: 71-81. DOI: 10.1016/j.myc.2018.09.003.
Gao Y, Zhao Z, Li J, Liu N, Jacquemyn H, Guo S, Xing X. 2020. Do fungal associates of co-occurring orchids promote seed germination of Gymnadenia conopsea? Mycorrhiza 30: 221-228. DOI: 10.1007/s00572-020-00943-1.
Heidari K, Mehrabi-Koushki M, Farokhinejad R. 2018. Curvularia mosaddeghii sp. nov., a novel species from Pleosporaceae. Mycosphere 9: 635-646. DOI: 10.5943/mycosphere/9/4/2.
Jiang J, Zhang K, Cheng S, Nie Q, Zhou SX, Chen Q, Zhou J, Zhen X, Li XT, Zhen TW, Xu M. 2019. Fusarium oxysporum KB-3 from Bletilla striata: An orchid mycorrhizal fungus. Mycorrhiza 29: 531-540. DOI: 10.1007/s00572-019-00904-3.
Li Y, Zhang X, Song X, Xu M, He K, Chi Y, Guo Z. 2025. Identification of a novel pathogen of peanut root rot, Ceratobasidium sp. AG-A and biocontrol potential of bacteria. J Fungi 11: 472. DOI: 10.3390/jof11070472.
Li Y, Kang Z, Zhang X, Sun P, Jiang X, Han Z. 2021. Mycorrhizal fungi of Cymbidium promote the growth of Dendrobium officinale by increasing stress tolerance. PeerJ 9: e12555. DOI: 10.7717/peerj.12555.
Ma XY, Mala B, Tan L. 2024. Two novel orchid mycorrhizal Tulasnella species and their ability to facilitate symbiotic seed germination. Research Square. DOI: 10.21203/rs.3.rs-5430346/v1.
Matloob AAH, Abid AY, Khadhair KZ. 2020. Efficiency of AMF and PGPR to control Fusarium chlamydosporum on date palm offshoots. Iraqi J Agric Sci 48 (2): 507. DOI: 10.36103/ijas.v48i2.418.
Matthapan L, Prasong W, Leeyaphan C, Bunyaratavej S, Lertrujiwanit K. 2018. A novel technique of aluminum multiplier slide culture for fungal identification. Siriraj Med J 70: 438-441. DOI: 10.14456/smj.2018.68.
Meng YY, Fan XL, Zhou LR, Shao SC, Liu Q, Selosse MA, Gao JY. 2019a. Symbiotic fungi undergo a taxonomic and functional bottleneck during germination of Dendrobium moniliforme. Symbiosis 79: 205-212. DOI: 10.1007/s13199-019-00647-x.
Meng YY, Shao SC, Liu SJ, Gao JY. 2019b. Do fungi associated with adult orchid roots support seed germination? A case study on Dendrobium exile. Glob Ecol Conserv 17: e00582. DOI: 10.1016/j.gecco.2019.e00582.
Meng YY, Zhang WL, Selosse MA, Gao JY. 2019c. Are fungi from adult orchid roots the best symbionts? Mycorrhiza 29: 541-547. DOI: 10.1007/s00572-019-00907-0.
Nuaimy MAA, Hawar SN. 2024. Antagonistic activity of endophytic fungi from Aloe vera leaves. Iraqi J Agric Sci 55: 63-79. DOI: 10.36103/ijas.v55iSpecial.1886.
Pereira ML, Carvalho JL, Lima JM, Barbier E, Bernard E, Bezerra JD, Souza-Motta CM. 2022. Richness of Cladosporium in a tropical bat cave with two new species. Mycol Prog 21: 345-357. DOI: 10.1007/s11557-021-01760-2.
Phillips RD, Reiter N, Peakall R. 2020. Orchid conservation: From theory to practice. Ann Bot 126: 345-362. DOI: 10.1093/aob/mcaa093.
Pitt JI, Hocking AD. 2013. Fungi and Food Spoilage. Springer, New York. DOI: 10.1007/978-0-387-92207-2.
Prawira DS, Yuliawati E, Purba E. 2019. Keanekaragaman anggrek di Hutan Bukit Kukus. Ekotonia 4: 57-64. DOI: 10.33019/ekotonia.v4i2.1785. [Indonesian]
Priwiratama H, Wiyono S, Hidayat SH, Wening S, Tondok ET. 2024. Identification and characterization of Curvularia causing leaf spot of oil palm seedlings. J Saudi Soc Agric Sci 2024. DOI: 10.1016/j.jssas.2024.10.003.
dos Reis JBA, Sturion Lorenzi A, Martins do Vale HM. 2022. Methods used in the study of endophytic fungi. Arch Microbiol 204: 675. DOI: 10.1007/s00203-022-03283-0.
Risman RDS, Sungthongwises K. 2025. Enhancing Candida tropicalis for the growth and yield of vegetable soybean. Iraqi J Agric Sci 56: 677-691. DOI: 10.36103/06qvnm20.
Rosanti D, Widianjaya RR. 2018. Morfologi Orchidaceae di Kebun Raya Liwa. Sainmatika 15: 84-90. DOI: 10.31851/sainmatika.v15i2.2371. [Indonesian]
Rungjindamai N, Jones EBG. 2024. Why so few Basidiomycota as endophytes? J Fungi 10: 67. DOI: 10.3390/jof10010067.
Sarsaiya S, Jain A, Jia Q, Fan X, Shu F, Chen Z, Zhou Q, Shi J, Chen J. 2020. Molecular identification of endophytic fungi and pathogenicity against Dendrobium. Intl J Mol Sci 21: 316. DOI: 10.3390/ijms21010316.
Sayem MA, Miah S, Hossain MM. 2024. Isolation and identification of endophytic fungi from roots of epiphytic orchids. Research Square. DOI: 10.21203/rs.3.rs-4463026/v1.
Schweiger JMI, Bidartondo MI, Gebauer G. 2018. Stable isotope signatures of underground seedlings reveal orchid carbon gain. Funct Ecol 32: 870-881. DOI: 10.1111/1365-2435.13042.
Sebastian J, Kathiresan D, Kuriakose G. 2021. Species diversity and abundance patterns of epiphytic orchids in Aralam Wildlife Sanctuary in Kerala, India. J Threatened Taxa 13: 19060-19069. DOI: 10.11609/jott.4852.13.8.19060-19069.
Soelistijono R, Utami DS, Priyatmojo A. 2017. Identifikasi Rhizoctonia dan Fusarium pada Ascocentrum miniatum. Biota 2: 7-13. DOI: 10.24002/biota.v2i1.1504. [Indonesian]
Soelistijono R, Utami DS, Daryanti, Faizin M, Dian R. 2020. Characterization of Rhizoctonia-like mycorrhizae in Dendrobium. Biodiversitas 21 (3): 1007-1011. DOI: 10.13057/biodiv/d210321.
Sofian S, Nurhasanah, Sopialena. 2025. Isolation and characterization of endophytic fungi antagonistic to Fusarium spp. Biodiversitas 26 (4): 1816-1825. DOI: 10.13057/biodiv/d260431.
Sundari D, Perdana NG, Mose W, Gutierrez-Marcos JF, Semiarti E. 2023. Detection of the AtRKD4 gene and somatic embryo induction in Phalaenopsis amabilis. J Trop Biodivers Biotechnol 8: 1-11. DOI: 10.22146/jtbb.71211.
Suryantini R, Wulandari RS, Kasiamdari RS. 2015. Orchid mycorrhiza: Identification of Rhizoctonia in West Kalimantan. Microbiol Indones 9: 157-162. DOI: 10.5454/mi.9.4.3.
Vierheilig H, Coughlan AP, Wyss U, Piche Y. 1998. Ink and vinegar staining technique for AM fungi. Appl Environ Microbiol 64: 5004-5007. DOI: 10.1128/AEM.64.12.5004-5007.1998.
Wang D, Gebauer G, Jacquemyn H, Zahn FE, Gomes SI, Lorenz J, van der Hagen H, Schilthuizen M, Merckx VS. 2023. Variation in mycorrhizal communities and mycoheterotrophy in Neottia ovata. Funct Ecol 37: 1948-1961. DOI: 10.1111/1365-2435.14354.
Wulandari FA, Sukamto DSS, Putri HR. 2025. Characterization of orchid root fungi as teaching material. J Penelitian Pendidikan IPA 11: 202-214. DOI: 10.29303/jppipa.vi1i5.10934.
Yang J, Ahmed W, Zhang J, Gao S, Wang Z, Yang H, Bai X, Luo K, Xu C, Ji G. 2024. Identification of Fusarium oxysporum causing leaf blight on Dendrobium chrysotoxum in Yunnan Province, China. Life 14: 285. DOI: 10.3390/life14030285.
Zhang Y, Li YY, Chen XM, Guo SX, Lee YI. 2020. Effect of different mycobionts on germination of Dendrobium officinale. Bot Stud 61 (1): 2. DOI: 10.1186/s40529-019-0278-6.
Zumri M, Daryanti, Soelistijono R. 2017. Isolasi dan identifikasi Rhizoctonia anggrek Vanda tricolor. Agrineca 17: 854-2813. DOI: 10.36728/afp.v17i1.560. [Indonesian]