Isolation and selection of maize plants rhizobacteria with the potential of entomopathogens against Spodoptera litura (Lepidoptera: Noctuidae)
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Abstract
Abstract. Nelly N, Khairul U, Putri AY, Hamid H, Syahrawati MY. 2020. Isolation and selection of maize plants rhizobacteria with the potential of entomopathogens against Spodoptera litura F (Lepidoptera: Noctuidae). Biodiversitas 21: 753-758. Spodoptera litura is a polyphagous pest that can be controlled with entomopathogen. Furthermore, Rhizobacteria derived from the rhizosphere of corn plants are expected to control this pest. This research, therefore, was conducted to obtain the entomopathogenic rhizobacteria to control S. litura derived from the rhizosphere of corn plants. Data sampling was carried out at corn plantations in West Pasaman Regency, while Isolation, selection, and testing of S. litura were conducted at the Biological Control Laboratory of the Plant Protection Department, Faculty of Agriculture, Andalas University. The method uses serial glowing techniques cultured on Nutrient Agar (NA) media, with the selected isolates tested for their ability as an entomopathogen against S. litura. The feed dip method given to S. litura larvae was used to test for rhizobacteria virulence determination with entomopathogen, used to observe the larvae mortality rate, percentage of pupae, and adult formed. Furthermore, observation of morphological and physiological characters was conducted on entomopathogenic rhizobacteria, with hypersensitive testing conducted on Mirabilis jalapa plants. The results of rhizobacterial isolation from corn rhizosphere obtained 12 entomopathogenic isolates. Deadly larvae of S. litura 51-91.11%, 0.00-8.89% pupae, and 0.00- 6.67% adult were formed. The gram test results obtained approximately 8;4 positive (+) and four negative isolates (-), respectively. Hypersensitivity test results found that one of 12 isolates caused necrotic reactions in Mirabilis jalapa. Therefore, a total of 11 rhizobacteria isolates are entomopathogenic and safe to be used as S. litura biological control agents.
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Barea, J.M., Pozo, M.J., Azcon, R., and Aguilar, C.A. 2005. Microbial co-operation in the rhizosphere. J. Exp. Bot. 56: 1761-1778
Castagnola, A. and Stock, S.P. 2014. Common Virulence Factors and Tissue Targets of Entomopathogenic Bacteria for Biological Control of Lepidoptera Pests. Insects. Vol 5; 139-166
Chapman, R.R. 1971. The Insect, Structure and Funtion. 2nd Edition. Elsevier. New York.
Dar GH, Sofi S, Padder SA, Aisha Kabli. 2018. Molecular characterization of rhizobacteria isolated from walnut (Juglans regia) rhizosphere in Western Himalayas and assessment of their plant growth promoting activities. Biodiversitas 19: 712-719.
Fatimah, Siti and T. Habazar and D. Arbain, A and Nurbailis, 2014. Characterization of Indigenous Rhizobacterial Isolates from Healthy Chilli Rhizosphere Capable of Inducing Resistance Against Anthracnose Disease (Colletotrichum Gloeosporioides). IJASET, 4 (3). pp. 72-76.
Khaeruni, A, Rahayu, Purnamaningrum, N.T. 2012. Isolasi Bacillus thuringiensis Berl. Dari tanah dan patogenesitasnya terhadap larva Crocidolomia binotalis Zell. Pada tanaman sawi (Brassica juncea L). Jurnal Agroteknos. 2(1): 21-27
Klement Z, K. Rudolph, D.C. Sand (Eds.), 1990. Methods in Phytobacteriology, Akademiai Kiado, Budapest, Hungary pp. 101-102.
Laoh, J.H., Puspita, F., Hendra. 2003. Kerentanan Larva Spodoptera litura F. Terhadap Virus Nuklear Polyhedrosis. Jurnal Natur Indonesia. 5(2): 145-151
Marwoto dan Suharsono. 2008. Strategi dan komponen teknologi pengendalian ulat grayak (Spodoptera litura Fabricius) pada Tanaman Kedelai. Balai Penelitia Tanaman Kacang-kacangan dan Umbi-umbian, Malang, Jurnal Litbang Pertanian. 27(4): 131-136
Rahma H, Arneti, Nofrianti S. 2018. In vitro selection of rhizobacteria for control Diplodia maydis cause of ear rot disease in maize. Pros Sem Nas Masy Biodiv Indon 4: 225-230
Rini, M.S., Rahadian, R., Hadi, M. Zulfiana, D. 2016. Uji Efikasi Beberapa Isolat Bakteri Entomopatogen terhadap Kecoa (Orthoptera) Periplaneta americana (L.) dan Blatella germanica (L.) dalam Skala Laboratorium. Jurnal Biologi. 5(2): 1-10
Rusmana, I., dan Hadioetomo, R.S, 1994. Isolasi Bacillus thuringiensis Berl. dari Peternakan Ulat Sutera dan Toksisitasnya terhadap Larva Crocidolomia binotalis Zell. dan Spodoptera litura F. Hayati. 1(1): 21-23
Salaki, C. 2011. Isolasi dan Karakterisasi Bakteri Indigenous (Bacillus cereus Frank) Sebagai Agensia Pengendalian Hayati Hama Kubis. Jurnal Ilmu Pertanian Eugenia. Vol 17 (1): 10-15
Salaki, C.L. Tarore, D., Manengkey, G. 2013. Prospek Pemanfaatan Biopestisida Bakteri Entomopatogenik Isolat Lokal Sebagai Agen Pengendali Hayati Hama Tanaman Sayuran. Jurnal Ilmu Pertanian Eugenia Vol. 19. No.1. hal 1-7
Sari, T.A. 2016. Uji Tingkat Kerapatan Populasi Bakteri Merah Serratia Sp. Terhadap Hama Spodoptera Litura F (Lepidoptera : Noctuidae). [Skripsi]. Padang. Fakultas Pertanian. Universitas Andalas. 47 hal.
Simatupang, DS. 2008. Berbagai Mikroorganisme Rizosfer Tanaman Pepaya (Carica papaya L.) di Pusat Kajian Buah-buahan Tropika (PKBT) IPB Desa Ciomas, Kecamatan Pasirkuda, Kabupaten Bogor, Jawa Barat. [Skripsi]. Departemen Proteksi Tanaman. Fakultas Pertanian. Institut Pertanian Bogor. 40 hal
Tampubolon, D.Y., Pangestiningsih, Y., Zahara, F., dan Manik, F. 2013. Uji Patogenesitas Bacillus thuringiensis dan Metarhizium anisopliae Terhadap Mortalitas Spodoptera litura Fabr (Lepidoptera : Noctuidae) Di Laboratorium. Jurnal Online Agroekoteknologi. 1(3) : 783-792
Tanada, Y. dan H.K, Kaya. 1993. Insect Pathology. Academic Press, San Diego, California
Untung, K. 1996. Pengantar Pengelolaan Hama Terpadu. Gadjah Mada University Press. Yogyakarta
Untung, K.2006. Pengantar pengelolaan hama terpadu. Gadjah mada university Press. Yogyakarta
Widyati, E. 2013. Memahami Interaksi Tanaman – Mikroba. Tekno Hutan Tanaman, 6(1): 13-20
Yanti,Y., Habazar, T., Resti, Z., dan Suhailita, D. 2013. Penampisan Isolat Rizobakteri Dari Perakaran Tanaman Kedelai Yang Sehat Untuk Pengendalian Penyakit Pustul Bakteri (Xanthomonas axonopodis Pv. Glycines). Jurnal HPT Tropika. 13(1) : 24-34
Yudiawati, E. 2016. Potensi Jamur Entomopatogen Beauveria bassiana (Balsamo) Vuillemin dan Metarhizium spp. Untuk Pengendalian Hama Walang Sangit (Leptocorisa acuta Thunb.). [Tesis]. Padang. Fakultas Pertanian. Universitas Andalas. 70 hal.
Zhu, W., M.M. Magbanua, F.F. White. 2000. Identification of two novel Hrp-Associated genes in the Hrp gene cluster of Xanthomonas oryzae pv. Oryzae. J. Bacteriol 182: 1844-1853
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