Short Communication: The presence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli on layer chicken farms in Blitar Area, Indonesia
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FRESHINTA JELLIA WIBISONO
https://orcid.org/0000-0001-9716-2230
BAMBANG SUMIARTO
http://orcid.org/0000-0003-1736-9691
TRI UNTARI
http://orcid.org/0000-0001-7370-5915
MUSTOFA HELMI EFFENDI
♥
DIAN AYU PERMATASARI
https://orcid.org/0000-0001-7423-3075
ADIANA MUTAMSARI WITANINGRUM
https://orcid.org/0000-0001-6071-1910
Abstract
Abstract. Wibisono FJ, Sumiarto B, Untari T, Effendi MH, Permatasari DA, Witaningrum AM. 2020. Short Communication: The presence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli on layer chicken farms in Blitar Area, Indonesia. Biodiversitas 21: 2667-2671. This study was aimed to determine the incidence of Extended-Spectrum Beta-Lactamase (ESBL) producing Escherichia coli on layer chicken in Blitar area. This was a cross-sectional study with a total of 205 cloacal swabs of layer chicken taken randomly. The sample was in isolation identification on MacConkey media and ESBL confirmation test produced by Escherichia coli was then carried out by the Double Disc Synergy Test (DDST) method and the VITEK® 2 Compact Automated System method. This study showed that 185 (90.24%) isolates of positive Escherichia coli from a total of 205 samples of cloacal swabs of the layer chicken. The incidence of ESBL-producing Escherichia coli in cloacal swabs on layer chicken with the Double Disc Synergy Test (DDST) method and the VITEK® 2 compact automatic method was 13 (7.03%). Results in this study indicated that layer chicken has potential as reservoir for spreading ESBL to public health and needs strict hygienic measures to prevent their transmission to humans.
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References
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Blaak H, Kruijf P De, Hamidjaja RA, Hoek AHAM Van, Maria A, Husman DR, Schets FM. 2014. Prevalence and characteristics of ESBL-producing E. coli in Dutch recreational waters influenced by wastewater treatment plants. Veterinary Microbiology 7: 448–459.
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Canton R, Novais A, Valverde A, Machado E, Peixe L, Baquero F, Coque TM. 2008. Prevalence and spread of extended spectrum beta lactamase-producing Enterobacteriaceae in Europe. Clinical Microbiol Rev 14: 144–153.
CLSI. 2017. M100 Performance Standards for Antimicrobial, 27th ed. Clinical and Laboratory Standards Institute, USA.
Duggal S, Gaind R, Tandon N, Deb M, Chugh T Das. 2012. Comparison of an Automated System with Conventional Identification and Antimicrobial Susceptibility Testing. international scholarly research notices microbiology.
Effendi MH, Harijani N, Yanestria SM. and Hastutiek P. 2018. Identification of Shiga toxin-producing Escherichia coli in raw milk samples from dairy cows in Surabaya, Indonesia. Philippine Journal of Veterinary Medicine 55: 109–114.
Effendi MH, Harijani N, Budiarto, Triningtya NP, Tyasningsih W, Plumeriastuti H. 2019. Prevalence of pathogenic Escherichia coli isolated from subclinical mastitis in East Java Province, Indonesia. Indian Veterinary Journal 96: 22–25.
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Gao L, Tan Y, Zhang X, Hu J, Miao Z. 2015. Emissions of Escherichia coli carrying extended spectrum ?-lactamase resistance from pig farms to the surrounding environment. International Journal of Environmental Research and Public Health 12: 4203–4213.
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Gundogan N, Avci E. 2013. Prevalence and antibiotic resistance of extended spectrum beta-lactamase (ESBL) producing Escherichia coli and Klebsiella species isolated from foods of animal origin in Turkey. African Journal of Microbiology Research 7: 4059–4064.
Handayani RS, Siahaan S, Herman MJ. 2017. Resistensi antimikroba dan penerapan kebijakan pengendalian di Rumah Sakit di Indonesia. Jurnal penelitian dan pengembangan pelayanan kesehatan 1: 131–140.
Huizen J, Seladi-Schulman J. 2017. Extended Spectrum Beta-Lactamases (ESBL): Infection and treatment. Medical News Today. MediLexicon, Intl., 26 Sep. 2017. Web.
26 Jun. 2018. www.medicalnewstoday.com/articles/319535.php
Kang D, Sinuraya RK, Rostinawati T, Abdulah R. 2017. Gene blaCTX?M mutation as risk factor of antibiotic resistance. Jurnal Farmasi Klini Indonesia 6: 135–152.
Kwoji ID, Musa JA, Daniel N, Mohzo DL, Bitrus AA. 2019. Extended-spectrum beta-lactamase-producing Escherichia coli in chickens from small-scale (backyard) poultry farms in Maiduguri, Nigeria. International Journal of One Health 5: 26–30.
Laube H, Friese A, Guerra B, Ro U. 2014. Transmission of ESBL/AmpC-producing Escherichia coli from broiler chicken farms to surrounding areas. Veterinary Journal 172: 519–527.
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Lukman DW, Sudarwanto MB, Purnawarman T, Latif H, Pisestyani H, Sukmawinata E, Akineden Ö. 2016. CTX-M-1 and CTX-M-55 producing Escherichia coli isolated from broiler feces in poultry slaughterhouse, Bogor, West Java Province. Global Advanced Research Journal of Medicine and Medical Sciences 5: 287–291.
Masruroh CA, Sudarwanto MB, Latif H. 2016. Tingkat kejadian Escherichia coli penghasil extended spectrum beta-lactamase yang diisolasi dari feses broiler di Kota Bogor. Jurnal Sain Veteriner 34: 42–49.
Mehdi Y, Létourneau-Montminy MP, Gaucher ML, Chorfi Y, Suresh G, Rouissi T, Brar SK, Côté C, Ramirez AA, Godbout S. 2018. Use of antibiotics in broiler production: Global impacts and alternatives. Animal Nutrition 4: 170–178.
Memish ZA, Balkhy HH, Shibl AM, Barrozo CP, Gray GC. 2004. Streptococcus pneumoniae in Saudi Arabia: Antibiotic resistance and serotypes of recent clinical isolates. International Journal of Antimicrobial Agents 23: 32–38.
Niasono AB, Latif H, Purnawarman T. 2019. Resistensi antibiotik terhadap bakteri Escherichia coli yang diisolasi dari peternakan ayam pedaging di Kabupaten Subang, Jawa Barat. Jurnal Veteriner 20: 187–195.
O’Hara CM. 2005. Manual and automated instrumentation for identification of Enterobacteriaceae and other aerobic Gram-Negative Bacilli. Clinical microbiology reviews 18: 147–162.
OIE. 2015. OIE?: Harmonisation of national antimicrobial resistance surveillance and monitoring programmes. Terrestrial Animal Health Code 1–6. www.oie.int
Overdevest I, Willemsen I, Rijnsburger M, Eustace A, Xu L, Hawkey P, Heck M, Savelkoul P, Vandenbroucke-grauls C, Zwaluw K Van Der, Huijsdens X, Kluytmans J. 2011. Genes of Escherichia coli in chicken meat and humans, the Netherlands. Emerging Infectious Diseases 17: 1216-1222.
Paterson DL, Bonomo RA. 2005. Extended spectrum beta-lactamases?: a clinical update. Clinical Microbiology Reviews 18: 657–686.
Putra ARS, Effendi MH, Koesdarto S, Tyasningsih W. 2019. Molecular identification of extended spectrum beta-lactamase (ESBL) producing Escherichia coli isolated from dairy cows in East Java Province, Indonesia. Indian Veterinary Journal 96: 26–30.
Reinthaler FF, Feierl G, Galler H, Haas D, Leitner E, Mascher F, Melkes A, Posch J, Winter I, Zarfel G, Marth E. 2010. ESBL-producing E. coli in Austrian sewage sludge. Water Research 44: 1981–1985.
Saliu BK, Sule IO, Agbabiaka TO. 2012. Comparative study of bacteria in the digestive tracts of chicken reared as free rangers and those reared in poultry. Biological and Environmental Sciences Journal for the Tropics 9: 26–39.
Santos LL dos, Moura RA, Aguilar-Ramires P, Castro AP De, Lincopan N. 2013. Current status of extended-spectrum ?-lactamase (ESBL)-producing Enterobacteriaceae in animals. Microbial pathogens and strategies for combating them: science, technology and education (A. Méndez-Vilas, Ed.) : 1600–07.
Savira M. 2014. Validitas metode konvensional modifikasi terhadap metode konvensional dan chromidTM ESBL untuk deteksi bakteri-bakteri penghasil extended-spectrum beta-lactamases. JIK 2: 81–90.
Seni J, Falgenhauer L, Simeo N, Mirambo MM, Imirzalioglu C, Matee M, Rweyemamu M, Chakraborty T, Mshana SE. 2016. Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids. Frontiers in Microbiology 7: 1–8.
Shoaib M, Kamboh AA, Sajid A, Mughal GA, Leghari RA, Malhi KK, Bughio SUD, Ali A, Alam S, Khan S, Ali S. 2016. Prevalence of extended spectrum beta-lactamase producing Enterobacteriaceae in commercial broilers and backyard chickens. Advances in Animal and Veterinary Sciences 4: 209–214.
Sugiartha IGE. 2016. Perbandingan hasil identifikasi metode Analytical Profile Index (API) dan tes kepekaan antibiotika konvensional dengan metode Technical Dedicated Reasonable (TDR)-300B. Universitas Airlangga. Surabaya. [Indonesian]
Utami ER. 2011. Antibiotika, resistensi, dan rasionalitas terapi. El Hayah 1: 191–198.
WHO. 2017. Stop using antibiotics in healthy animals to prevent the spread of antibiotic resistance. www.who.int
Yusha’u M, Umar, MI. 2016. Phenotypic detection of extended spectrum beta lactamases in bacterial isolates from meat products sold within Kaduna Metropolis in Nigeria. Proceeding of International Conference On Biodiversity Society for Indonesian Biodiversity. Sebelas Maret University, Surakarta, 14-16 January 2016. [Indonesian]
Zheng H, Zeng Z, Chen S, Liu Y, Yao Q, Deng Y, Chen X, Lv L, Zhuo C, Chen Z, Liu J. 2012. Prevalence and characterisation of CTX-M beta-lactamases among Escherichia coli isolates from healthy food animals in China. International Journal of Antimicrobial Agents 39: 305–310.
Anggraini D, Hasanah U, Savira M, Andrini F, Irawan D, Prima R. 2018. Prevalence and susceptibility profile of ESBL-producing Enterobacteriaceae in Arifin Achmad General Hospital Pekanbaru. Jurnal Kedokteran Brawijaya 30: 47–52.
Bellet C. 2018. Drug resistance and the dynamics of livestock farm practices. Journal of Rural Studies 63: 57–64.
Biomerieux. 2017. AST and Resistance Detection, Antibiotic Suceptibility Testing bioMérieux Industry website. www.biomerieux-diagnostics.com
Biutifasari V. 2018. Extended spectrum beta–lactamase (ESBL). Oceana Biomedicina Journal 1: 1–6.
Blaak H, Kruijf P De, Hamidjaja RA, Hoek AHAM Van, Maria A, Husman DR, Schets FM. 2014. Prevalence and characteristics of ESBL-producing E. coli in Dutch recreational waters influenced by wastewater treatment plants. Veterinary Microbiology 7: 448–459.
Brooks GF, Carroll KC, Butel J, Morse SA, Mietzner T. 2013. Medical Microbiology, 26th ed, Medical Microbiology. Mc Graw Hill. United States.
Brower CH, Mandal S, Hayer S, Sran M, Zehra A, Patel SJ, Kaur R, Chatterjee L, Mishra S, Das BR, Singh P, Singh R, Gill JPS, Laxminarayan R. 2017. The prevalence of extended spectrum beta lactamase-producing multidrug-resistant Escherichia coli in poultry chickens and variation according to farming practices in Punjab, India. Environmental Health Perspectives 125: 1–10.
Canton R, Novais A, Valverde A, Machado E, Peixe L, Baquero F, Coque TM. 2008. Prevalence and spread of extended spectrum beta lactamase-producing Enterobacteriaceae in Europe. Clinical Microbiol Rev 14: 144–153.
CLSI. 2017. M100 Performance Standards for Antimicrobial, 27th ed. Clinical and Laboratory Standards Institute, USA.
Duggal S, Gaind R, Tandon N, Deb M, Chugh T Das. 2012. Comparison of an Automated System with Conventional Identification and Antimicrobial Susceptibility Testing. international scholarly research notices microbiology.
Effendi MH, Harijani N, Yanestria SM. and Hastutiek P. 2018. Identification of Shiga toxin-producing Escherichia coli in raw milk samples from dairy cows in Surabaya, Indonesia. Philippine Journal of Veterinary Medicine 55: 109–114.
Effendi MH, Harijani N, Budiarto, Triningtya NP, Tyasningsih W, Plumeriastuti H. 2019. Prevalence of pathogenic Escherichia coli isolated from subclinical mastitis in East Java Province, Indonesia. Indian Veterinary Journal 96: 22–25.
Estiningsih D, Puspitasari I, Nuryastuti T. 2016. Identification of multidrug resistant organisms infection in Neonatal Intensive Care Unit (UNIT). Jurnal Manajemen dan Pelayanan Farmasi 6: 243–248.
Etikaningrum, Iwantoro S. 2017. Kajian residu antibiotika pada produk ternak unggas di Indonesia. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan 05: 29–33.
Gao L, Tan Y, Zhang X, Hu J, Miao Z. 2015. Emissions of Escherichia coli carrying extended spectrum ?-lactamase resistance from pig farms to the surrounding environment. International Journal of Environmental Research and Public Health 12: 4203–4213.
Guentzel MN. 1996. Medical Microbiology?: Chapter 26 Escherichia, Klebsiella, Enterobacter, Serratia, Citrobacter, and Proteus, 4th ed, Medical Microbiology. University of Texas Medical Branch at Galveston, Texas.
Gundogan N, Avci E. 2013. Prevalence and antibiotic resistance of extended spectrum beta-lactamase (ESBL) producing Escherichia coli and Klebsiella species isolated from foods of animal origin in Turkey. African Journal of Microbiology Research 7: 4059–4064.
Handayani RS, Siahaan S, Herman MJ. 2017. Resistensi antimikroba dan penerapan kebijakan pengendalian di Rumah Sakit di Indonesia. Jurnal penelitian dan pengembangan pelayanan kesehatan 1: 131–140.
Huizen J, Seladi-Schulman J. 2017. Extended Spectrum Beta-Lactamases (ESBL): Infection and treatment. Medical News Today. MediLexicon, Intl., 26 Sep. 2017. Web.
26 Jun. 2018. www.medicalnewstoday.com/articles/319535.php
Kang D, Sinuraya RK, Rostinawati T, Abdulah R. 2017. Gene blaCTX?M mutation as risk factor of antibiotic resistance. Jurnal Farmasi Klini Indonesia 6: 135–152.
Kwoji ID, Musa JA, Daniel N, Mohzo DL, Bitrus AA. 2019. Extended-spectrum beta-lactamase-producing Escherichia coli in chickens from small-scale (backyard) poultry farms in Maiduguri, Nigeria. International Journal of One Health 5: 26–30.
Laube H, Friese A, Guerra B, Ro U. 2014. Transmission of ESBL/AmpC-producing Escherichia coli from broiler chicken farms to surrounding areas. Veterinary Journal 172: 519–527.
Leboffe MJ, Pierce BE. 2011. A Photograpic Atlas for the Microbiology Laboratory, 4th ed. Morton Publishing Company, United States of America.
Lukman DW, Sudarwanto MB, Purnawarman T, Latif H, Pisestyani H, Sukmawinata E, Akineden Ö. 2016. CTX-M-1 and CTX-M-55 producing Escherichia coli isolated from broiler feces in poultry slaughterhouse, Bogor, West Java Province. Global Advanced Research Journal of Medicine and Medical Sciences 5: 287–291.
Masruroh CA, Sudarwanto MB, Latif H. 2016. Tingkat kejadian Escherichia coli penghasil extended spectrum beta-lactamase yang diisolasi dari feses broiler di Kota Bogor. Jurnal Sain Veteriner 34: 42–49.
Mehdi Y, Létourneau-Montminy MP, Gaucher ML, Chorfi Y, Suresh G, Rouissi T, Brar SK, Côté C, Ramirez AA, Godbout S. 2018. Use of antibiotics in broiler production: Global impacts and alternatives. Animal Nutrition 4: 170–178.
Memish ZA, Balkhy HH, Shibl AM, Barrozo CP, Gray GC. 2004. Streptococcus pneumoniae in Saudi Arabia: Antibiotic resistance and serotypes of recent clinical isolates. International Journal of Antimicrobial Agents 23: 32–38.
Niasono AB, Latif H, Purnawarman T. 2019. Resistensi antibiotik terhadap bakteri Escherichia coli yang diisolasi dari peternakan ayam pedaging di Kabupaten Subang, Jawa Barat. Jurnal Veteriner 20: 187–195.
O’Hara CM. 2005. Manual and automated instrumentation for identification of Enterobacteriaceae and other aerobic Gram-Negative Bacilli. Clinical microbiology reviews 18: 147–162.
OIE. 2015. OIE?: Harmonisation of national antimicrobial resistance surveillance and monitoring programmes. Terrestrial Animal Health Code 1–6. www.oie.int
Overdevest I, Willemsen I, Rijnsburger M, Eustace A, Xu L, Hawkey P, Heck M, Savelkoul P, Vandenbroucke-grauls C, Zwaluw K Van Der, Huijsdens X, Kluytmans J. 2011. Genes of Escherichia coli in chicken meat and humans, the Netherlands. Emerging Infectious Diseases 17: 1216-1222.
Paterson DL, Bonomo RA. 2005. Extended spectrum beta-lactamases?: a clinical update. Clinical Microbiology Reviews 18: 657–686.
Putra ARS, Effendi MH, Koesdarto S, Tyasningsih W. 2019. Molecular identification of extended spectrum beta-lactamase (ESBL) producing Escherichia coli isolated from dairy cows in East Java Province, Indonesia. Indian Veterinary Journal 96: 26–30.
Reinthaler FF, Feierl G, Galler H, Haas D, Leitner E, Mascher F, Melkes A, Posch J, Winter I, Zarfel G, Marth E. 2010. ESBL-producing E. coli in Austrian sewage sludge. Water Research 44: 1981–1985.
Saliu BK, Sule IO, Agbabiaka TO. 2012. Comparative study of bacteria in the digestive tracts of chicken reared as free rangers and those reared in poultry. Biological and Environmental Sciences Journal for the Tropics 9: 26–39.
Santos LL dos, Moura RA, Aguilar-Ramires P, Castro AP De, Lincopan N. 2013. Current status of extended-spectrum ?-lactamase (ESBL)-producing Enterobacteriaceae in animals. Microbial pathogens and strategies for combating them: science, technology and education (A. Méndez-Vilas, Ed.) : 1600–07.
Savira M. 2014. Validitas metode konvensional modifikasi terhadap metode konvensional dan chromidTM ESBL untuk deteksi bakteri-bakteri penghasil extended-spectrum beta-lactamases. JIK 2: 81–90.
Seni J, Falgenhauer L, Simeo N, Mirambo MM, Imirzalioglu C, Matee M, Rweyemamu M, Chakraborty T, Mshana SE. 2016. Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids. Frontiers in Microbiology 7: 1–8.
Shoaib M, Kamboh AA, Sajid A, Mughal GA, Leghari RA, Malhi KK, Bughio SUD, Ali A, Alam S, Khan S, Ali S. 2016. Prevalence of extended spectrum beta-lactamase producing Enterobacteriaceae in commercial broilers and backyard chickens. Advances in Animal and Veterinary Sciences 4: 209–214.
Sugiartha IGE. 2016. Perbandingan hasil identifikasi metode Analytical Profile Index (API) dan tes kepekaan antibiotika konvensional dengan metode Technical Dedicated Reasonable (TDR)-300B. Universitas Airlangga. Surabaya. [Indonesian]
Utami ER. 2011. Antibiotika, resistensi, dan rasionalitas terapi. El Hayah 1: 191–198.
WHO. 2017. Stop using antibiotics in healthy animals to prevent the spread of antibiotic resistance. www.who.int
Yusha’u M, Umar, MI. 2016. Phenotypic detection of extended spectrum beta lactamases in bacterial isolates from meat products sold within Kaduna Metropolis in Nigeria. Proceeding of International Conference On Biodiversity Society for Indonesian Biodiversity. Sebelas Maret University, Surakarta, 14-16 January 2016. [Indonesian]
Zheng H, Zeng Z, Chen S, Liu Y, Yao Q, Deng Y, Chen X, Lv L, Zhuo C, Chen Z, Liu J. 2012. Prevalence and characterisation of CTX-M beta-lactamases among Escherichia coli isolates from healthy food animals in China. International Journal of Antimicrobial Agents 39: 305–310.
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