Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure
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Abstract. Ambarwati A, Wahyuono S, Moeljopawiro S, Yuwono T. 2020. Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure. Biodiversitas 21: 3380-3390. The goal of the research was to determine the antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and predict their chemical structure of the bioactive compounds based on the AntiSMASH analysis of the whole genome sequencing. Streptomyces sp. CRB46 was isolated from Cyperus rotundus L. rhizosphere in the Cemoro Sewu highland, Indonesia (1920 meters above sea level). The results showed that the supernatant ethyl acetate (EAS) extract could inhibit 11 of 12 tested microorganisms with a diameter of the inhibition zone ranging from 17-35 mm (paper disc method). The supernatant extract was found more effective in inhibiting microorganisms than the ethyl acetate extract of pellets (EAP). The EAP extract was only able to inhibit the growth of seven tested microorganisms (16-26 mm). The results of LCMS analysis showed that crude extract of EAS consisted of 20 mixtures of organic compounds. Based on FTIR analysis, 16 functional groups were found in the EAS extracts. In addition, based on AntiSMASH analysis, it was also found that Streptomyces sp. CRB46 produced eight classes of bioactive compounds, but only six compounds have been identified as their chemical structures. It can, therefore, be concluded that Streptomyces sp. CRB46 has the potential to produce bioactive compounds that can be used for microbial infection treatment.
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References
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Arifuzzaman M, Khatun MR, Rahman H. 2010. Isolation and screening of Actinomycetes from Sundarbans soil for antibacterial activity. Afr J Biotechnol 9(29): 4615-4619.
Blin K, Shaw S, Steinke K, Villebro R, Ziemert N, Lee SY, Medema MH, Weber T. 2019. AntiSMASH 5.0: updates to the secondary metabolite genome mining pipeline. Nucleic Acids Res 47(W1):W81–W87.
Charousová I, Javoreková S, Wink J. 2015. Isolation and Characterization of Streptomyces rishiriensis (VY31) with antibiotic activity against various pathogenic microorganisms. J Microbiol Biotechnol Food Sci 4:23-27.
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Gulve RM, Deshmukh AM. 2012. Antimicrobial activity of the marine Actinomycetes. Int Multidiscip Res J 2(3):16-22.
Haque MA, Sarker AK, Islam MS, Anwar-Ul-Islam M. 2015. Antibacterial potential of ethyl-acetate extracts of marine Streptomyces spp. AIAH-10 against drug-resistant Escherichia coli. Br Microbiol Res J 7(3): 143-150.
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Shetty PR, Buddana SK, Tatipamula VB, Naga YVV, Ahmad J. 2014. Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity. Braz J Microbiol 45(1):303-312.
Sunaryanto R, Marwoto R. 2010. Marine Actinomycetes screening of Banten West Coast and their antibiotics purification. Biodiversitas 11(4):2085-4722.
Tangjitjaroenkun, J., 2018. Evaluation of antioxidant, antibacterial, and gas chromatography analysis of ethyl acetate extract of Streptomyces omiyaensis SCH2. Asian J Pharm Clin Re.11(7):271-6.
Ullah I, Arshed M, Noureen U, Chuadhry MJI, Jadoon MA. 2012. Isolation of Streptomyces from the sediments of selected thermal springs of northern Pakistan and its intrinsic susceptibility and resistance. Records: Zoological Survey of Pakistan 21:47-50.
Anderson AS, Wellington EMH. 2001. The taxonomy of Streptomyces and related genera. Int. J. Syst Evol Microbiol 51:797-814.
Apsari PP, Budiarti S, Wahyudi AT. 2019. Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria. Biodiversitas 20(5):2085-4722.
Arifuzzaman M, Khatun MR, Rahman H. 2010. Isolation and screening of Actinomycetes from Sundarbans soil for antibacterial activity. Afr J Biotechnol 9(29): 4615-4619.
Blin K, Shaw S, Steinke K, Villebro R, Ziemert N, Lee SY, Medema MH, Weber T. 2019. AntiSMASH 5.0: updates to the secondary metabolite genome mining pipeline. Nucleic Acids Res 47(W1):W81–W87.
Charousová I, Javoreková S, Wink J. 2015. Isolation and Characterization of Streptomyces rishiriensis (VY31) with antibiotic activity against various pathogenic microorganisms. J Microbiol Biotechnol Food Sci 4:23-27.
Farnet CM, Zazopoulos E. 2005. Improving drug discovery from microorganisms. In: Zhang, L., Demain, A.L. (eds). Natural Products. Humana Press. Springer: 95-106.
Gulve RM, Deshmukh AM. 2012. Antimicrobial activity of the marine Actinomycetes. Int Multidiscip Res J 2(3):16-22.
Haque MA, Sarker AK, Islam MS, Anwar-Ul-Islam M. 2015. Antibacterial potential of ethyl-acetate extracts of marine Streptomyces spp. AIAH-10 against drug-resistant Escherichia coli. Br Microbiol Res J 7(3): 143-150.
Herdini H, Mubarika S, Hariwiyanto B, Wijayanti N, Hosoyama A, Yamazoe A, Nojiri H, Widada J. 2017. Secondary bioactive metabolite gene clusters identification of anticandida-producing Streptomyces sp. GMR22 isolated from Wanagama forest as revealed by the genome mining approach. Indonesian J Pharm 28(1): 26-33.
Hikida T, Shimizu K, Umeta T, Utsumi M, Sugiura N. 2011. Influence of environmental factor on musty odor production by Streptomyces coelicolor A3(2). 14th World Lake Conference Lakes, Rivers, Groundwater & Coastal area: understanding Lingkages Austin. 31 October-4 November 2011. Austin Convention Center, Austin, Texas USA.
Ignacimuthu S, Devadass BJ, Paulraj MG, Theoder AS, Dhabi NA. 2017. Identification of antimicrobial compounds from Streptomyces sp. isolated from western ghats soil in Tamil Nadu. EC Microbiol 8(4): 222-231.
Korn-Wendisch F, Kutzner HJ. 1992. The family Streptomycetaceae. In the prokaryotes, A handbook on the biology of bacteria: ecophysiology, isolation, identification, applications. Second Edition. (A. Balows, H. G. Truper, M. Dworkin, W. Harder, and Karl-Heinz Schleifer. Eds). Springer-Verlag, New York, Berlin, Heidelberg, London, Paris, Tokyo, Hong Kong, Barcelona, & Budapest.
Lazzarini A, Cavaletti L, Toppo G, Marinelli F. 2000. Rare genera of Actinomycetes as a potential producer of new antibiotics. Anton Leeuw Int J G 78(3-4):399-405.
Madigan MT, Martinko JM, Parker J. 2003. Brock biology of microorganisms. Tent Edition. Prentice-Hall, USA.
Naikpatil SV, Rathod JL. 2011. Antimicrobial and cytotoxic activity of Actinomycetes from Karwar coast, west coast of India. World J Sci Technol 1(1):07-10.
Nedialkova D, Naidenova M. 2005. Screening the antimicrobial activity of Actinomycetes strains isolated from Antarctica. J Cult Collect 4:29-35.
Okami Y, Hotta K. 1988. Search and discovery of new antibiotics. In Actinomycetales in Biotechnology. (M. Goodfellow, S. T. Williams, & M. Mordarski, Eds). Academic Press Limited, London.
Pan Y, Xu L, Cao W, Yin S, Wang Z, Zhou Q. 2009. Actinomycetes and earthy-musty odorous compounds in brackish fishponds in Tianjin, China. Water Sci Technol 59(6):1185-1194.
Patil R, Jeyaskaran G, Shanmugan SA. 2016. Occurrence and antibacterial activity of Actinomycetes isolated from marine samples from Thoothukkudi, Tamil Nadu, on the east coast of India, against fish pathogens. IJABR 6(3):338-347.
Rajan BM, Kannabiran K. 2014. Extraction and Identification of Antibacterial Secondary Metabolites from Marine Streptomyces sp. VITBRK2. Int J Mol Cell Med 3(3):130–137.
Retnowati Y, Moeljopawiro S, Djohan TS, Soetarto ES. 2018. Antimicrobial activities of Actinomycetes isolated from rhizospheric soil on different mangrove forests of Torosiaje, Gorontalo, Indonesia. Biodiversitas 19(6):2196-2203.
Shetty PR, Buddana SK, Tatipamula VB, Naga YVV, Ahmad J. 2014. Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity. Braz J Microbiol 45(1):303-312.
Sunaryanto R, Marwoto R. 2010. Marine Actinomycetes screening of Banten West Coast and their antibiotics purification. Biodiversitas 11(4):2085-4722.
Tangjitjaroenkun, J., 2018. Evaluation of antioxidant, antibacterial, and gas chromatography analysis of ethyl acetate extract of Streptomyces omiyaensis SCH2. Asian J Pharm Clin Re.11(7):271-6.
Ullah I, Arshed M, Noureen U, Chuadhry MJI, Jadoon MA. 2012. Isolation of Streptomyces from the sediments of selected thermal springs of northern Pakistan and its intrinsic susceptibility and resistance. Records: Zoological Survey of Pakistan 21:47-50.
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