Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines

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KHUSNUL FADHILAH
SUBAGUS WAHYUONO
PUJI ASTUTI

Abstract

Abstract. Fadhilah K, Wahyuono S, Astuti P. 2021. Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines. Biodiversitas 22: 3743-3748. Lansium domesticum (Fam. Meliaceae), a tropical fruit (local name, Duku), has been reported to have various biological activities. At the moment we emphasize on searching compounds to have cytotoxic activity from the peel of L. domesticum. The study was initiated by extraction with EtOAc followed by fractionation that was monitored by thin-layer chromatography (TLC) and cytotoxicity against T-47D and HepG2 cells. The EtOAc extract of the sample was triturated with n-hexane to give n-hexane soluble (A) n-hexane insoluble fractions (B). The B fraction was fractionated by vacuum column chromatography using gradient solvent composition of n-hexane: acetone to give 18 fractions. According to TLC similarity pictures, fractions were combined to give 6 fractions (I-VI). Isolation was performed using preparative TLC, and the cytotoxic assay was performed using MTT method. The isolated compound was identified as a sesquiterpene having one aldehyde functional group, based on spectroscopic data.The isolated compound displayed cytotoxic activity on T-47D (IC50, 48.58 + 0.96 µg/mL) and HepG2 (IC50, 127.45 + 25.76 µg/mL).

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References
Ahmed, S., A. J. Al-Rehaily, M. S. Ahmad, M. Yousaf, M. Nur-e-Alam, J. Thomas, S. I. Khan, and I. A. Khan. 2018. “Cytotoxic and Antiinflammatory Activities of the Chemical Constituents Isolated from Baccharoides Schimperi DC.” South African Journal of Botany 114: 9–13. https://doi.org/10.1016/j.sajb.2017.10.007.
Akihisa, Toshihiro, Xin Pan, Yasuhiro Nakamura, Takashi Kikuchi, Nami Takahashi, Masahiro Matsumoto, Eri Ogihara, Makoto Fukatsu, Kazuo Koike, and Harukuni Tokuda. 2013. “Limonoids from the Fruits of Melia Azedarach and Their Cytotoxic Activities.” Phytochemistry 89: 59–70. https://doi.org/10.1016/j.phytochem.2013.01.015.
An, Fa Liang, Xiao Bing Wang, Hui Wang, Zhong Rui Li, Ming Hua Yang, Jun Luo, and Ling Yi Kong. 2016. “Cytotoxic Rocaglate Derivatives from Leaves of Aglaia Perviridis.” Scientific Reports 6 (January): 6–17. https://doi.org/10.1038/srep20045.
Bahuguna, Ashutosh, Imran Khan, Vivek K. Bajpai, and Sun Chul Kang. 2017. “MTT Assay to Evaluate the Cytotoxic Potential of a Drug.” Bangladesh Journal of Pharmacology 12 (2): 115–18. https://doi.org/10.3329/bjp.v12i2.30892.
Bosco, Alessandra, and Roy M. Golsteyn. 2017. “Emerging Anti-Mitotic Activities and Other Bioactivities of Sesquiterpene Compounds upon Human Cells.” Molecules 22 (3). https://doi.org/10.3390/molecules22030459.
El-Desoky, Ahmed H.H., Keisuke Eguchi, Hikaru Kato, Naoki Kishimoto, Shogo Misumi, Takashi Watanabe, and Sachiko Tsukamoto. 2020. “Chamaejasmins, Cytotoxic Guaiane Sesquiterpenes from the Root of Stellera Chamaejasme L.” Fitoterapia 146 (August): 104714. https://doi.org/10.1016/j.fitote.2020.104714.
Fadhilah, Khusnul, Subagus Wahyuono, and Puji Astuti. 2020. “A Bioactive Compound Isolated from Duku (Lansium Domesticum Corr) Fruit Peels Exhibits Cytotoxicity against T47D Cell Line.” F1000Research 9: 3. https://doi.org/10.12688/f1000research.21072.1.
Kee, M. E., H. E. Khoo, C. M. Sia, and H. S. Yim. 2015. “Fractionation of Potent Antioxidative Components from Langsat (Lansium Domesticum) Peel.” Pertanika Journal of Tropical Agricultural Science 38 (1): 103–12.
Kuete, Victor. 2018. “Ardisinol III, a Naturally Occurring Alkenylmethylresorcinol Displayed Cytotoxic Effects in Carcinoma Cells.” Investigational Medicinal Chemistry and Pharmacology 1 (2): 1–6. https://doi.org/10.31183/imcp.2018.00014.
Manosroi, Aranya, Pensak Jantrawut, Mathukorn Sainakham, Worapaka Manosroi, and Jiradej Manosroi. 2012. “Anticancer Activities of the Extract from Longkong (Lansium Domesticum) Young Fruits.” Pharmaceutical Biology 50 (11): 1397–1407. https://doi.org/10.3109/13880209.2012.682116.
Marfori, Eufrocinio C, Shin Ichiro Kajiyama, Ei-Ichiro Fukusaki, and Akio Kobayashi. 2015. “Lansioside D, a New Triterpenoid Glycoside Antibiotic from the Fruit Peel of Lansium Domesticum Correa.” ~ 140 ~ Journal of Pharmacognosy and Phytochemistry 3 (5): 140–43.
Matsumoto, Takahiro, Takahiro Kitagawa, Stephen Teo, Yuuka Anai, Risa Ikeda, Daisuke Imahori, Haji Sapuan Bin Ahmad, and Tetsushi Watanabe. 2018. “Structures and Antimutagenic Effects of Onoceranoid-Type Triterpenoids from the Leaves of Lansium Domesticum.” Journal of Natural Products. https://doi.org/10.1021/acs.jnatprod.8b00341.
Mayanti, Tri. 2009. Kandungan Kimia Dan Bioaktivitas Tanaman Duku. UNPAD Press. UNPAD PRESS.
P., Klungsupya, Suthepakul N., Muangman T., Rerk-Am U., and Thongdon-A. J. 2015. “Determination of Free Radical Scavenging, Antioxidative DNA Damage Activities and Phytochemical Components of Active Fractions from Lansium Domesticum Corr. Fruit.” Nutrients.
Passos, Michel de S., Almir R.de Carvalho, Samyra I. Boeno, Lorena de L.Glória das Virgens, Sanderson Dias Calixto, Thatiana Lopes Biá Ventura, Elena Lassounskaia, Raimundo Braz-Filho, and Ivo J.Curcino Vieira. 2019. “Terpenoids Isolated from Azadirachta Indica Roots and Biological Activities.” Revista Brasileira de Farmacognosia 29 (1): 40–45. https://doi.org/10.1016/j.bjp.2018.12.003.
Rudiyansyah, Andi Hairil Alimuddin, Masriani, Rini Muharini, and Peter Proksch. 2018. “New Tetranortriterpenoids, Langsatides A and B from the Seeds of Lansium Domesticum Corr. (Meliaceae).” Phytochemistry Letters 23 (December 2017): 90–93. https://doi.org/10.1016/j.phytol.2017.11.019.
Susilo, Susilo, and Rizkia Suciati. 2016. “Studies of Morphological and Secondary Metabolites Variaty of Mosses (Bryophyta) in Cibodas, West Java.” International Journal of Advanced Research 4 (12): 1397–1402. https://doi.org/10.21474/ijar01/2536.
Thinh, Ngo Sy, Nguyen Thi Bich Thu, Tran Minh Ngoc, Nguyen Minh Khoi, Bui Huu Tai, Phan Van Kiem, Chau Van Minh, et al. 2020. “Cytotoxic Sesquiterpene Glucosides from Fissistigma Pallens.” Phytochemistry 172 (January): 112255. https://doi.org/10.1016/j.phytochem.2019.112255.
Xu, Wen Jun, Jun He Li, Miao Miao Zhou, Jie Luo, Kai Li Jian, Xiao Meng Tian, Yuan Zheng Xia, Lei Yang, Jun Luo, and Ling Yi Kong. 2020. “Toonasindiynes A-F, New Polyacetylenes from Toona Sinensis with Cytotoxic and Anti-Inflammatory Activities.” Fitoterapia 146 (June): 104667. https://doi.org/10.1016/j.fitote.2020.104667.
Zulfikar, Nurul Kamila Putri, Sofa Fajriah, Muhammad Yusuf, Rani Maharani, Jamaludin Al Anshori, Unang Supratman, and Tri Mayanti. 2020. “New Onoceranoid Triterpenes from Lansium” 1 (Figure 1): 2–6. https://doi.org/10.3390/M1157.

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