Bioassay-guided isolation of the antioxidant constituent from Kaempferia rotunda L.
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Aryantini D, Astuti P, Yuniarti N, Wahyuono S. 2023. Bioassay-guided isolation of the antioxidant constituent from Kaempferia rotunda L. Biodiversitas 24: 3641-3648. Kaempferia rotunda L. contains various phytochemical compounds with various biological activities and has been widely utilized in traditional medicine. This research focused on exploring bioactive compounds in the ethanol extract of Kaempferia rotunda using radical scavenging (1,1-Diphenyl-2-picrylhydrazyl) bioassay-guided isolation. Initially, the powdered material of K. rotunda was macerated with 70% ethanol and filtered, and the filtrate was evaporated in vacuo to make concentrated ethanol extract. The concentrated ethanol extract was then triturated gradually with increasing polarity of solvents (n-hexane, ethyl acetate, ethanol) to give n-hexane (HSF), ethyl acetate (EASF), ethanol (ESF), and residue (ISF) fractions. Each fraction was tested by DPPH bioassay with quercetin as the positive control to determine the active fraction. The active fraction (ESF) was further fractionated by Vacuum Liquid Chromatography (VLC) using a mobile gradient phase starting from ethyl acetate 100%, acetone 100%, and methanol 100% tomake3 sub-fractions (F1, F2, F3),respectively. The DPPH radical scavenging bioassay showed that F1 was the most active, containing bioactive compound detected by TLC visualized by DPPH. Based on spectroscopic and literature data comparison, this compound was isolated, purified, and identified ascrotepoxide. Crotepoxide displayed IC50of 38.91±0.59 (ABTS), 47.45±0.60 (DPPH),and 26.74±1.23 (FRAP) µg/mL.
##plugins.themes.bootstrap3.article.details##
References
Aryantini, Dyah, Puji Astuti, Nunung Yuniarti, and Subagus Wahyuono. 2022. Extraction and Isolation of Phytochemicals from Kaempferia Rotunda Linn. (White Turmeric) for Pharmacological Application: A Review. Tropical Journal of Natural Product Research 6(9):1359–66. doi: 10.26538/TJNPR/V6I9.2.
Atun, Sri. 2014. Phytochemical Of Kaempferia Plant And Bioprospecting For Cancer Treatment. Pp. 1–9 in Proceeding of International Conference On Research, Implementation And Education Of Mathematics And Sciences 2014. Yogyakarta State University.
Atun, Sri, Retno Arianingrum, Eddy Sulistyowati, and Nurfina Aznam. 2013. Isolation and Antimutagenic Activity of Some Flavanone Compounds from Kaempferia Rotunda. International Journal of Chemical and Analytical Science 4(1):3–8. doi: 10.1016/j.ijcas.2013.03.004.
Blois, Marsden S. 1958. Antioxidant Determinations by the Use of a Stable Free Radical. Nature 181:1199–1200. doi: 10.1038/1811199a0.
Cadenas, Enrique. 1997. Basic Mechanisms of Antioxidant Activity. BioFactors 6(4):391–97. doi: 10.1002/biof.5520060404.
Desmiaty, Y., W. Winarti, A. M. Nursih, H. Nisrina, and G. Finotory. 2018. Antioxidant and Antielastase Activity of Kaempferia Rotunda and Curcuma Zedoaria. Research Journal of Chemistry and Environment 22(special I):95–98.
Desta, Zelalem Yibralign, and Norbert Sewald. 2017. Isolation of Non-Cytotoxic Crotepoxide from the Root Bark of Croton Macrostachyus and the Reassignment of Its Structure. Bulletin of the Chemical Society of Ethiopia 31(3):465–69. doi: 10.4314/bcse.v31i3.10.
Diastuti, Hartiwi, Mochammad Chasani, and Suwandri. 2020. Antibacterial Activity of Benzyl Benzoate and Crotepoxide from Kaempferia Rotunda L. Rhizome. Indonesian Journal of Chemistry 20(1):9–15. doi: 10.22146/ijc.37526.
Fadhilah, Khusnul, Subagus Wahyuono, and Puji Astuti. 2021. Fractions and Isolated Compounds from Lansium Domesticum Fruit Peel Exhibited Cytotoxic Activity against T-47D and HepG2 Cell Lines. Biodiversitas Journal of Biological Diversity 22(9):3743–48. doi: 10.13057/BIODIV/D220918.
Gelaw, Habtamu, Legesse Adane*, Yinebeb Tariku, and Asrat Hailu. 2012. Isolation of Crotepoxide From Berries of Croton Macrostachyus and Evaluation of Its Anti-Leishmanial Activity. Journal of Pharmacognosy and Phytochemistry 1(4):15–24.
Halvorsen, Bente L., Kari Holte, Mari C. W. Myhrstad, Ingrid Barikmo, Erlend Hvattum, Siv Fagertun Remberg, Anne Brit Wold, Karin Haffner, Halvard Baugerød, Lene Frost Andersen, O. Moskaug, David R. Jacobs, and Rune Blomhoff. 2002. A Systematic Screening of Total Antioxidants in Dietary Plants. Journal of Nutrition 132(3):461–71. doi: 10.1093/jn/132.3.461.
Hussain, Nisreen, and Anil Kumar. 2012. Reactive Oxygen Species and Natural Antioxidants: A Review. Advances in Bioresearch 3(4):164–75.
Jothy, Subramanion Lachumy, Tamio Saito, Jagat Rakesh Kanwar, Subramaniam Kavitha, Leow Chiuan, Yeng Chen, Leong Yin-hui, and Sreenivasan Sasidharan. 2017. Bioassay-Guided Isolation and Antioxidant Evaluation of Rutin from Leaf of Polyalthia Longifolia. 05(01).
Kupchan, S. M., R. J. Hemingway, P. Coggon, A. T. Mcphail, and G. A. Sim. 1968. Crotepoxide, a Novel Cyclohexane Diepoxide Tumor Inhibitor from Croton Macrostachys. Journal of the American Chemical Society 90(11):2982–83.
Larson, Richard A. 1988. The Antioxidants of Higher Plants. Phytochemistry 27(4):969–78. doi: 10.1016/0031-9422(88)80254-1.
Liang, Ningjian, and David D. Kitts. 2015. Antioxidant Property of Coffee Components: Assessment of Methods That Define Mechanisms of Action. (November 2014). doi: 10.3390/molecules191119180.
Lotulung, Puspa D. N., Minarti, L. B. S. Kardono, and K. Kawanishi. 2008a. Antioxidant Compound from the Rhizomes of Kaempferia Rotunda L. Pakistan Journal of Biological Sciences 11(20):2447–50. doi: 10.3923/pjbs.2008.2447.2450.
Lotulung, Puspa D. N., Minarti, L. B. S. Kardono, and K. Kawanishi. 2008b. Antioxidant Compound from the Rhizomes of Kaempferia Rotunda L. Pakistan Journal of Biological Sciences 11(20):2447–50. doi: 10.3923/pjbs.2008.2447.2450.
Malahayati, Nura, Tri Wardhani Widowati, and Anita Febrianti. 2018. Total Phenolic, Antioxidant and Antibacterial Activities of Curcumin Extract of Kunci Pepet (Kaempferia Rotunda L). Research Journal of Pharmaceutical Biological and Chemical Sciences 9(3):129–35.
Malviya, Neelesh, and Sapna Malviya. 2017. Bioassay Guided Fractionation-an Emerging Technique Influence the Isolation, Identification and Characterization of Lead Phytomolecules. International Journal of Hospital Pharmacy (January 2017). doi: 10.28933/ijhp-2017-07-0901.
Marjoni, M. R., and A. Zulfisa. 2017. Antioxidant Activity of Methanol Extract/Fractions of Senggani Leaves (Melastoma Candidum D. Don). Pharmaceutica Analytica Acta 08(08):1–6. doi: 10.4172/2153-2435.1000557.
Mohanty, J. Priya, L. K. Nath, Bhuyan Nihar, and G. Mariappan. 2008. Evaluation of Antioxidant Potential of Kaempferia Rotunda Linn. Indian Journal of Pharmaceitical Science 70(3):362–64.
Mudjiono, Isni Herawati, Sis Munawaroh, and Sukari. 2014. Kearifan Lokal Orang Madura Tentang Jamu Untuk Kesehatan Ibu Dan Anak. Balai Pelestarian Nilai Budaya Yogyakarta.
Pancharoen, Orasa, Pittaya Tuntiwachwuttikul, and Walter C. Taylor. 1996. Cyclohexane Diepoxides from Kaempferia Rotunda. Phytochemistry 43(1):305–8. doi: 10.1016/0031-9422(96)00218-X.
Panyakaew, Jukreera, Sirinrat Chalom, Siriwoot Sookkhee, Aroonchai Saiai, Nopakarn Chandet, Puttinan Meepowpan, Praput Thavornyutikarn, and Pitchaya Mungkornasawakul. 2020. Kaempferia Sp. Extracts as UV Protecting and Antioxidant Agents in Sunscreen. Journal of Herbs, Spices and Medicinal Plants 27(1):1–20. doi: 10.1080/10496475.2020.1777614.
Pizzino, Gabriele, Natasha Irrera, Mariapaola Cucinotta, Giovanni Pallio, Federica Mannino, Vincenzo Arcoraci, Francesco Squadrito, Domenica Altavilla, and Alessandra Bitto. 2017. Oxidative Stress: Harms and Benefits for Human Health. Oxidative Medicine and Cellular Longevity 2017. doi: 10.1155/2017/8416763.
Prasad, Sahdeo, Vivek R. Yadav, Chitra Sundaram, Simone Reuter, Padmanabhan S. Hema, Mangalam S. Nair, Madan M. Chaturvedi, and Bharat B. Aggarwal. 2010. Crotepoxide Chemosensitizes Tumor Cells through Inhibition of Expression of Proliferation, Invasion, and Angiogenic Proteins Linked to Proinflammatory Pathway. Journal of Biological Chemistry 285(35):26987–97. doi: 10.1074/jbc.M110.121061.
Rafi, Mohamad, Nadya Meitary, Dewi Anggraini Septaningsih, and Maria Bintang. 2020. View of Phytochemical Profile And Antioxidant Activity Of Guazuma Ulmifolia Leaves Extracts Using Different Solvent Extraction. Indonesian Journal of Pharmacy 31(2):171–80.
Ramana, Kota V, Aramati B. M. Reddy, N. V. Ravi Kumar Majeti, and Sharad S. Singhal. 2018. Therapeutic Potential of Natural Antioxidants. Oxidative Medicine and Cellular Longevity 2018:9471051. doi: 10.1155/2018/9471051.
Rotich, Winnie, Eduard Mas-Claret, Nicholas Sadgrove, Anastasia Guantai, Guillermo F. Padilla-González, and Moses K. Langat. 2022. HIV-1 Integrase Inhibitory Effects of Major Compounds Present in CareVidTM: An Anti-HIV Multi-Herbal Remedy. Life 12(3). doi: 10.3390/life12030417.
Rusiliati, Taty, Shinta Njotosiswoyo, and Ratna Djamil. 2003. Isolasi Dan Uji Aktivitas Antibakteri Rimpang Kunyit Putih (Kaempferia Rotunda L). Pp. 335–40 in Prosiding lokakarya dan seminar nasional pengembangan dan pemanfaatan obat dari bahan tumbuhan. Perpustakaan BBTPPI Semarang: Lembaga Penelitian Universitas Diponegoro Semarang.
Shalaby, Emad A., and Sanaa M. M. Shanab. 2013. Comparison of DPPH and ABTS Assays for Determining Antioxidant Potential of Water and Methanol Extracts of Spirulina Platensis. Indian Journal of Marine Sciences 42(5):556–64.
Stevenson, Philip C., Nigel C. Veitch, and Monique S. J. Simmonds. 2007. Polyoxygenated Cyclohexane Derivatives and Other Constituents from Kaempferia Rotunda L. Phytochemistry 68(11):1579–86. doi: 10.1016/j.phytochem.2007.03.015.
Suciyati, S. W., Sukrasno, N. F. Kurniati, and I. K. Adnyana. 2021. Antioxidant and Anti-Inflammatory Activity of Red Ginger (Zingiber Officinale Roscoe Var. Sunti Val) Essential Oil Distillation Residues. Egyptian Journal of Chemistry 64(9):5031–35. doi: 10.21608/EJCHEM.2021.52477.3083.
Tang, Sook Wah, Mohd Aspollah Sukari, Bee Keat Neoh, Yunie Soon Yu Yeap, Ahmad Bustamam Abdul, Nurolaini Kifli, and Gwendoline Cheng Lian Ee. 2014. Phytochemicals from Kaempferia Angustifolia Rosc. and Their Cytotoxic and Antimicrobial Activities. BioMed Research International 2014. doi: 10.1155/2014/417674.
Thi Phuong, Ngo, Do Thi Thanh Huyen, Bui Thi Hai Hoa, Le Ngoc Hung, and Le Minh Ha. 2020. A New Polyoxygenated Cyclohexene From the Rhizomes of Globba Pendula Roxb. Natural Product Communications 15(4):3–6. doi: 10.1177/1934578X20922382.
Utami, Prapti. 2008. Buku Pintar Tanaman Obat: 431 Jenis Tanaman Penggempur Aneka Penyakit - Redaksi AgroMedia - Google Buku. edited by D. damayanti. Tangerang: Agromedia.
Wahyuningsih, Wahyuningsih, Miranti Miranti, Hilwan Yuda Teruna, and Titania Tjandrawati Nugroho. 2021. Isolation of a High Antioxidant Non-Toxic Polar Fraction from Garcinia Mangostana Fruit Pericarp by Reverse Phase Column Chromatography. Jurnal Kimia Sains Dan Aplikasi 24(1):15–21. doi: 10.14710/JKSA.24.1.15-21.
Yeap, Yunie Soon Yu, Nur Kartinee Kassim, Rou Chian Ng, Gwendoline Cheng Lian Ee, Latifah Saiful Yazan, and Khalid Hamid Musa. 2017. Antioxidant Properties of Ginger (Kaempferia Angustifolia Rosc.) and Its Chemical Markers. International Journal of Food Properties 20(1):S1158–72. doi: 10.1080/10942912.2017.1286508.
Most read articles by the same author(s)
- AMBARWATI AMBARWATI, SUBAGUS WAHYUONO, SUKARTI MOELJOPAWIRO, TRIWIBOWO YUWONO, Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 7 (2020)
- AMBARWATI AMBARWATI , SUBAGUS WAHYUONO, SUKARTI MOELJOPAWIRO, LANGKAH SEMBIRING, TRIWIBOWO YUWONO, Antimicrobial activities and phylogenetic study of bacteria associated with Cyperus rotundus rhizosphere from Cemoro Sewu Plateau, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 8 (2019)
- KHUSNUL FADHILAH, SUBAGUS WAHYUONO, PUJI ASTUTI, Fractions and isolated compounds from Lansium domesticum fruit peel exhibited cytotoxic activity against T-47D and HepG2 cell lines , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 9 (2021)
- Andita Eltivitasari, Rahmawati Rahmawati, Baiq Maylinda Gemantari, Fitra Romadhonsyah, Arief Nurrochmad, Subagus Wahyuono, Puji Astuti, Effect of light exposure on secondary metabolites production of an endophytic fungus Arthrinium rasikravindrae and its antioxidant and anticancer activities , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 6 (2021)
- NANANG FAKHRUDIN, FATIYA FARIH MUFINNAH, MUHAMMAD FAISHAL HUSNI, ARIEF EKA WARDANA, ERADHIAN IRMA WULANDARI, ARBIE RISTANTO PUTRA, DJOKO SANTOSA, ARIEF NURROCHMAD, SUBAGUS WAHYUONO, Screening of selected Indonesian plants for antiplatelet activity , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 12 (2021)
- KHUSNUL FADHILAH , SUBAGUS WAHYUONO, PUJI ASTUTI, Short Communication: Terpenoids isolated from Lansium domesticum fruit peel induce apoptosis and cell cycle arrest in T-47D cell lines , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 2 (2022)
- MAE SRI HARTATI WAHYUNINGSIH, SUBAGUS WAHYUONO, DJOKO SANTOSA, JUSAIN SETIADI, SOEKOTJO SOEKOTJO, SITI . MUSLIMAH WIDIASTUTI, RITA RAKHMAWATI, DINAR SARI CAHYANINGRUM WAHYUNI, Exploration of Central Kalimantan’s forest plants as bioactive compound resources , Biodiversitas Journal of Biological Diversity: Vol. 9 No. 3 (2008)
- CATERINA SISKADEWI LAY, IKRA AGUSTINA, PUJI ASTUTI, TRIANA HERTIANI, Cytotoxic screening of endophytic fungi associated with Catharanthus roseus , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 5 (2023)