Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste
##plugins.themes.bootstrap3.article.sidebar##
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Lingga R, Budiarti S, Rusmana I, Wahyu AT. 2020. Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste. Biodiversitas 21: 3234-3241. Escherichia coli is known as a pathogenic contaminant bacteria in hospital wastewater hazardous to humans and the environment. Concerns about the emergence of chlorine- and antibiotic-resistant bacteria increase the urgency to find an alternative strategy to control pathogenic bacteria in hospital wastewater. One of the alternatives is using lytic bacteriophage. This study aimed to isolate, characterize, and examine the efficacy of lytic bacteriophage against pathogenic Escherichia coli from hospital wastewater. It isolated, characterized (plaque morphology, host range, virion electron micrograph, and sensitivity to temperature, pH, and chlorine treatments), and tested the efficacy of lytic bacteriophages in controlling pathogenic E. coli isolated from hospital wastewater. Five phages were successfully obtained, all of which had clear plaques (lytic phage character). Based on host range assay, most of the phages could lyse all tested E. coli strains but not for other species. Electron micrograph photography revealed that the phages belonged to Myoviridae. The phages showed stability in high temperature, broad-ranged pH, and high concentrations of chlorine treatments. Assay on phages efficacy suggested that the phages are capable of significantly reducing the E. coli population both in sterilized and non-sterilized wastewater. The combination of phage treatment and chlorine was more effective than single phage treatment. The efficacy test revealed that phage application in wastewater had the best result seen from cocktail treatment and a combination of phage treatment and chlorine. These results suggested that the phage can be a potential candidate for disinfection purposes.
##plugins.themes.bootstrap3.article.details##
Anastasi EM, Matthews B, Stratton HM, Katouli M. 2012. Pathogenic Escherichia coli Found in Sewage Treatment Plants and Environmental Waters. Appl. Environ. Microbiol. 78 (6): 5536-5541.
Atamer Z, Dietrich J, Neve H, Heller KJ, Hinrichs J. 2010. Influence of the suspension media on the thermal treatment of mesophilic lactococcal bacteriophages. Int. Dairy J. 20: 408-414.
Aslan A, Cole Z, Bhattacharya A, Oyibo O. 2018. Presence of Antibiotic-resistant Escherichia coli in wastewater treatment plant effluents utilized as water reuse for irrigation. Water. 805(10):1-11.
Basdew IH, Laing MD. 2014. Stress sensitivity assays of bacteriophages associated with Staphylococcus aureus, causal organism of bovine mastitis. Afr J Microbiol Res. 8(2): 200-210.
Budiarti S, Pratiwi RH, Rusmana I. 2011. Infectivity of lytic phage to Enteropathogenic Escherichia coli from diarrheal patients in Indonesia. J US-China Med Sci. 8(5): 273-282.
Budiarti S, Lingga R, Rusmana I, Wahyudi AT. 2018. Antibiotics resistant Escherichia coli from hospital liquid waste. JABS. 21(1): 36-40.
Budiarti S, Sunarti RN, Listya SAP, Rusmana I. 2019. Biological characteristics and lytic effectiveness of phages isolated from domestic wastewater against indigenous Salmonella spp. J Environ Biol. 40: 53-60.
Chowdhury S. 2012. Heterotrophic bacteria in drinking water distribution system review. Environ Monit Assess. 184: 6087-6137.
Diwan V, Tamhankar AJ, Khandal RK. 2010. Antibiotics and antibiotic-resistant bacteria in waters associated with hospital in Ujjain, India. BMC Public Health. 10: 414.
Fernández L, Gutiérrez D, García P, Rodríguez A. 2019. The perfect bacteriophage for therapeutic applications—a quick guide. Antibiotics. 8 (126). doi:10.3390/antibiotics8030126.
Gallet R, Kannoly S, Wang I. 2011. Effects of bacteriophage traits on plaque formation. BMC Microbiol. 11(181): 1-16.
Gu J, Liu X, Li Y, Han W, Lei L, Yang Y, Zhao H, Gao Y, Song J, Lu R, Sun C, Feng X. 2012. A method for generation phage cocktail with great therapeutic potential. Plos One. 7(3): 31698-31706.
Herawati D, Yuntarso A. 2017. Penentuan dosis kaporit sebagai desinfektan dalam menyisihkan konsentrasi ammonium pada air kolam renang. J Sain Health. 1(2): 13-22.
Hu F, Chen S, Xu X, Gou Y, Liu Y, Zhu D, Zhang Y. 2011. Emergence of Carbapenem-resistant clinical Enterobacteriaceae isolates from a teaching hospital in Shanghai, China. J Med Microbiol. 61: 132-136.
Kanna RJ. 2015. Inactivation of viruses in water by chlorination using bacteriophages as model organisms. [Thesis]. Swedish University of Agricultural Sciences, Uppsala. [Sweden] www. stud.epsilon.slu.se.
León M, Bastías R. 2015. Virulence reduction in bacteriophage resistant bacteria. Front. Microbiol. 6: 343.
Rattanachaikunsopon P, Phumkhachorn P. 2012. Bacteriophage PPST1 isolated from hospital wastewater, a potential therapeutic agent against drug resistant Salmonella enterica subsp. enterica serovar Typhi. Intechopen. DOI: 10.5772/30446.
Skurnik D, Clermont O, Guillard T, Launay A, Danilchanka O, Pons S, Diancourt L, Lebreton F, Kadlec K, Roux D, Jiang D, Dion S, Aschard H, Denamur M, Cywes-Bentley C, Schwarz S, Tenaillon O, Andremont A, Picard B, Mekalanos J, Brisse S, Denamur E. 2015. Emergence of Antimicrobial-Resistant Escherichia coli of Animal Origin Spreading in Humans. Mol. Biol. Evol. 33(4):898-914.
Taj MK, Ling JX, Bing LL, Qi Z, Taj I, Hassani TM, Samreen Z, Yunlin W. 2014. Effect of dilution, temperature and ph on the lysis activity of T4 phage against E. coli BL21. J Animal Plant Sci. 24(4): 1252-1255.
Tenaillon O, Skurnik D, Picard B, Denamur E. 2010. The population genetics of commensal Escherichia coli. Nat Rev Microbiol. 8:207-217.
Van den Beld MJ, Reubsaet FA. 2012. Differentiation between Shigella, Enteroinvasive Escherichia coli (EIEC) and noninvasive Escherichia coli. Eur. J. Clin. Microbiol. Infect. Dis. 31: 899–904.
Woodford N, Turton JF, Livermore DM. 2011. Multiresistant Gramnegative bacteria: the role of high-risk clones in the dissemination of antibiotic resistance. FEMS Microbiol Rev. 35:736-755.
Zhong Q, Carratalà A, Ossola R, Bachmann V, Kohn T. 2017. Cross-resistance of uv- or chlorine dioxide-resistant echovirus 11 to other disinfectants. Front Microbiol. 8: 1-12.
Most read articles by the same author(s)
- RINI SUSILOWATI, DIETRIECH G. BENGEN, MAJARIANA KRISANTI, HEDI INDRA JANUAR, IMAN RUSMANA, Temporal and spatial distribution of plankton community in three Indonesian salt pond environments , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 3 (2023)
- DEMMA ZILBA BUDIMAN, WIDYA ESTI PURWANINGTYAS, JEPRI AGUNG PRIYANTO, IVAN PERMANA PUTRA, ABDJAD ASIH NAWANGSIH, ARIS TRI WAHYUDI, Glucanase activity produced by rhizospheric Streptomyces tritolerans ARJ 32 and Streptomyces collinus ARJ 38 and the analysis of their encoding genes , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 10 (2023)
- DELFIANI ANGGIAS PUTRI, RIKA INDRI ASTUTI, ARIS TRI WAHYUDI, The potency of yellow pigment extract from the marine bacterium Pseudomonas oryzihabitans SAB E-3 as an antioxidant agent , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 6 (2024)
- IDA AYU PUTU SURYANTI, MARIA INDAH PURNAMASARI, CAHYA PRIHATNA, IMAN RUSMANA, ARIS TRI WAHYUDI, ANTONIUS SUWANTO, Characterization of endophytic bacterial isolates from oil palm (Elaeis guineensis) seedlings and ramets for their plant growth promoting potential , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 10 (2024)
- DOVA KELVIN MESRIAN, RIKA INDRI ASTUTI, MUHAMMAD EKA PRASTYA, ARIS TRI WAHYUDI, Pyocyanin derived from the marine sponge-associated bacterium, Pseudomonas aeruginosa P1.S9, has the potential as antibacterial , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 11 (2024)