Short Communication: Acute toxicity study of plantaricin from Lactobacillus plantarum S34 and its antibacterial activity
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Abstract. Ahaddin AY, Budiarti S, Mustopa AZ, Darusman HS, Triratna L. 2021. Short Communication: Acute toxicity study of plantaricin from Lactobacillus plantarum S34 and its antibacterial activity. Biodiversitas 22: 227-232. Lactobacillus plantarum S34 belongs to the Lactobacilli class produces a bacteriocin called plantaricin. Bacteriocins are well known as active compounds that inhibit bacterial growth. This study was conducted to determine the antimicrobial activity of plantaricin S34 and its safety profile in the ddY mouse animal models. Plantaricin 34 from the crude extract was identified using Tricine SDS-PAGE. Antimicrobial activity was observed using disk diffusion against EPEC K1.1, S. aureus, S. typhosa, S typhimurium, and Proteus sp. The safety assessment showed that crude extract of plantaricin S34 did not cause any abnormalities to experimental mice even after being administrated with 5000 mg/kg BB. The identification of plantaricin S34 showed an active molecule at 7.34 kDa and had an activity to inhibit the pathogens used in this study. The blood analysis showed that the hematological and biochemical blood properties were in the normal range. Histopathological examination showed no damage to the intestine, liver, and kidneys. Thus, the crude extract of plantaricin S34 is active as an antimicrobial agent without any toxicity effects.
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References
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Zhang X, Yang W, Lei L, Yunlu W, Nan S, Xiangmei Z, Pinglan L. 2015. Twopeptide bacteriocin PlnEF causes cell membrane damage to Lactobacillus plantarum. Biochim et Biophys Acta 1858 (2016) 274–280.
Arief II, BSL Jenie, T Suryati, G Ayuningtyas, A Fuziawan. 2015. Plantaricin IIA-1A5 from Lactobacillus plantarum IIA-1A5 displays bactericidal activity against Staphylococcus aureus. Beneficial Microbes 6(4): 603–613.
Budiarti S. 2011. Antibiotic resistance Escherichia coli isolated from faecal of healthy human. Journal of International Environmental Application and Science 6(3): 359–364.
Budiarti S, Pratiwi RH, Rusmana I. 2011. Inefectivity of lytic phage to enteropathogenic Escherichia coli from diarrheal patients in Indoneisa. J US-China Med Sci 8(5): 273–282.
Dosler S, Mataraci E. 2013. In vitro pharmacokinetics of antimicrobial cationic peptides alone and combination with antibiotics against maethicillin resistant Staphylococcus aureus biofilm. Peptides 49(2013): 53–58.
Hanny ELL, Mustopa AZ, Bdiarti S, Darusman HS, Ningrum RA, Fatimah. 2019. Efficacy, toxicity study, and antioxidant properties of plantaricin E and F recombinants against enteropathogenic Escherichia coli K1.1 (EPEC K1.1). Mol Biol Rep 46(2019): 6501–6512.
Hata T, Tanaka R, Ohmomo S. 2010. Isolation and characterization of plantaricin ASM1: a new bacteriocin produced by Lactobacillus plantarum A-1. Int J Food Microbiol 137(2010):94-99.
Jozala AF, de Andrade MS, de Arauz LJ, Pessoa A, Penna TCV. 2007. Nisin production utilizing skimmed milk aiming to reduce process cost. Applied Biochemistry and Biotechnology 515–528.
Karaoglu A, Faruk A, Kilic SS, Kilic AO. 2003. Antimicrobial activity and characteristics of bacteriocins produced by vaginal lactobacilli. Turk J Med Sci 33: 7-13.
Martono M, Satino S. 2014. Severity renal function detection through critical changes glomerulular filtration rate in hemodialysis patients. Jurnal Ners 9(1): 43–48.
Mustopa AZ. 2013. Isolation and characterization of Lactobacillus plantarum S34 from Indonesian traditional food. [Disertation]. Dankook University,Yongin. [South Korea]
Mustopa AZ, Kusdianawati, Fatimah, Umami RN, Budiarto RB, Danuri H. 2016. Cloning and expression of plantaricin E and F genes of Lactobacillus plantarum S34 isolated from indonesian tradisional-fermented meat (Bekasam). IFJR 23: 762–769.
Nur HS. 2005. Pembentukan senyawa asam organik oleh isolat bakteri asam laktat pada media ekstrak daging buah durian (Durio zibethinus Murr). Bioscientiae 2(1): 15–24.
Omar R, Yadav A. 2018.The remarkable cationic peptides: a boon to pharmaceuical science. JPPS 21(1): 60-72.
Pal G, Sheela S. 2014b. Cloning and heterologous expression of plnE, -F, -J and – K genes derived from soil metagenome and purification of active plantaricin peptides. Appl Microbiol Biotechnol 98(3): 1441–1447.
Prophet ED, Mills B, Arrington BJ, Sobin LH. 1992. Armed Force Institute of Pathology–Laboratory Methods in Histotechnology. American Registry of Pathology, Washington DC.
Rogne P, Haugen C, Fimland G, Nissen-Meyer J, Kristiansen PE. 2009. Three-dimensional structure of the two-peptide bacteriocin plantaricin JK. Peptides 30: 1613–1621.
Santos EW, Dalila CO, DC de Oliveira, Araceli H, GB da Silva, JS de Oliveira Beltran, Maristela T, Amanda RC, Silvania MPN, Ricardo AF, Primavera B. 2016. Hematological and biochemical reference value for C57BL/6 Swiss Webster and balb/c mice. Braz. J. Vet. Res. Anim. Sci 53: 138-145.
Schagger H. 2006. Tricine SDS-PAGE. Nature Protocols 1(1): 16-22
Sharma A, Srivastava S. 2014. Anti-Candida activity of two-peptide bacteriocins, plantaricins (Pln E/F and J/K) and their mode of action. Fungal Biology. 118(2014) 264e275.
Umami RN, Mustopa AZ, Sukmarini L, Danuri H, Putri AS, Wibowo KDA. 2017. Cloning, exprerssion, and partial purification of plantaricin W locus produced by Lactobacillus plantarum S34. Berita Biologi. 16: 59–67.
Utami ER. 2011. Antibiotika, Resistensi, dan Rasionalitas Terapi. Fakultas Sains dan Tekhnologi UIN Maliki, Malang.
World Health Organization. 2017. Glonal priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics. WHO PPL Short Summary. https://www.who.int/
Xie Y, An H, Hao Y, Qin Q, Huang Y, Luo Y, Zhang L. 2011. Characterization of an anti-Listeria bacteriocin produced by Lactobacillus plantarum LB-B1 isolated fdrom koumiss, a traditionally fermented dairy product from China. J Food Control 22: 1027-1031.
Zhang X, Yang W, Lei L, Yunlu W, Nan S, Xiangmei Z, Pinglan L. 2015. Twopeptide bacteriocin PlnEF causes cell membrane damage to Lactobacillus plantarum. Biochim et Biophys Acta 1858 (2016) 274–280.
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