Short Communication: Histopathology of red chilli fruit (Capsicum annuum) infected with Colletotrichum acutatum of Java, Indonesia isolates
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
##plugins.themes.bootstrap3.article.main##
JUNI SAFITRI MULJOWATI
♥
LOEKAS SOESANTO
https://orcid.org/0000-0002-6501-4177
LAURENTIUS HARTANTO NUGROHO
https://orcid.org/0000-0001-7887-6860
Abstract
Abstract. Muljowati JS, Soesanto L, Nugroho LH. 2021. Short Communication: Histopathology of red chilli fruit (Capsicum annuum) infected with Colletotrichum acutatum of Java, Indonesia isolates. Biodiversitas 22: 874-880. Colletotrichum acutatum isolates from Malang, Temanggung, Kulonprogo, Brebes, Garut, and Pandeglang in Indonesia varied in their ability to produce pigments. In the present study, the histopathological status of red chilli was investigated during the early phase of infection by C. acutatum Java isolate. The results included a description of the histopathological features of red chillies (Capsicum annuum) in the early phase of infection by C. acutatum isolates and the relationship between the origin of the isolates and the time of onset of infection. The red chilli fruits were inoculated with fungal conidia suspension and then the histopathology of chilli fruits was observed at 0 hours (uninfected control), 8 hours, 16 hours, 24 hours, and 32 hours after inoculation. The results showed that C. acutatum isolates from Kulonprogo caused host damage within 8 hours, while the other isolates (from Malang, Brebes, Garut and Pandeglang) began to produce similar symptoms at 16 hours or 24 hours. This study revealed for the first time that pathogenic activity begins at the onset of infection, and the resulting anatomical damage to red chillies begins at different times.
##plugins.themes.bootstrap3.article.details##
References
Ajmal M, Abida A, Anum A, Shaista A, Brian GN. 2016. Stem histopathology of sesame seedlings infected with Alternaria alternata. Microscopy Research 4:11–19.
Anand T, Ramanujam B, Thiruvengadam R, Gandhi K, Manikam R, Govindasamy S. 2008. Production of cell wall degrading enzymes and toxins by Colletotrichum capsici and Alternaria alternata causing fruit rot of chillies. J of Plant Protection Research 48:437–51.
Arroyo FT, Javier M, Gregorio G, Berta DS, Carmen B, Maria P, Cesar B, Fernando R. 2005. Ultrastructure of the early stages of Colletotrichum acutatum infection of strawberry tissue. Canadian J of Botany 83:491-500.
Bellincampi D, Felice C, Vincenzo L. 2014. Plant cell wall dynamics and wall-related susceptibility in planta-pathogen interactions. Frontiers in Plant Science 5:1–9.
Curry KJ, Maritza A, Jana BA, Barbara JS. 2002. Strawberry anthracnose:Histopathology of Colletotrichum acutatum and C. fragariae. Phytopathology 92:1055–63.
Desire MH, Fotso B, Muyang RF, Chiatoh TA, Ndoumou D. 2014. Enzymes and qualitative phytochemical screening of endophytic fungi isolated from Lantana camara Linn. J of Appl Bio and Biotech 2:1–6.
Devi PA, Prakasam V. 2014. Histopathology studies of snthracnose and powdery mildew diseases in chillies. World J of Biology and Biological Sci 2:98–101.
Elsenman HC, Arturo C. 2012. Synthesis and assembly of fungal melanin. Appl.Microbiol Biotechnol 93:931–40.
Gautam AK. 2014. The Genera Colletotrichum: An incitant of numerous new plant diseases in India. J on New Biological Reports 3:9–21.
Geetha S, Kokkaiah I, Sinthia G, Palanichamy M. 2016. Preliminary phytochemical screening of different solvent extracts and stem of Crataeva religiosa Hook Frost. Int J of Current Microbiol and Appl Sci 5:116–22.
González E, Turner BS, James CC. 2006. Clarification of the etiology of Glomerella leaf spot and bitter rot of apple caused by Colletotrichum spp. based on morphology and genetic, molecular, and pathogenicity tests. Phytopathology 96(9):982–92.
Guidarelli M, Carbone F, Mourgues F, Perrotta G, Rosati C, Bertolini P, Baraldi E. 2011. Colletotrichum acutatum interactions with unripe and ripe strawberry fruits and differential responses at histological and transcriptional levels. Plant Pathology 60:685–97.
Hakim A, Muhamad S, Widodo. 2014. Ketahanan penyakit antraknosa terhadap cabai lokal dan cabai introduksi. Bul. Agrohorti 2(1):31–36.
Liu GY, Victor N. 2009. Color me bad: Microbial pigments as virulence factors. Trends in Microbiology 17:406–13.
Muljowati JS, Loekas S, Laurentius HN. 2020. Production of pectinase enzymes by Colletotrichum acutatum Simmonds causing anthracnose in red chilli ( Capsicum annuum, L).P. 012036 in IOP Conference Series: Earth and Enviromnental Science, volume 593.
Pandey A, Pandey BK, Muthukumar M, Yadava LP, Chauhan UK. 2012. Histopathological study of infection process of Colletotrichum gloeosporioides Prnz and Sacc. on Mangifera indica L. Plant Pathology Journal 11:18–24.
Pardo EM, Grellet CF, Salazar SM, Castagnaro AP, Ricci JCD, Arias ME. 2012. Histopathology of the resistance to Colletotrichum gloeosporioides of wild strawberries and species related to commercial strawberry. Australian J of Crop Sci 6:1147–53.
Pusztahelyi T, Imre JH, István P. 2015. Secondary metabolites in fungus-plant interactions. Frontier in Plant Sci 6:1–23.
Saxena A, Richa R, Vijai KG, Harikesh BS. 2016. Chilli anthracnose: The epidemiology and management. Frontiers in Microbiology 7:1–18.
Silva SAM, Rosana R, Leandro SAGs, Cláudia PS, Cíntia SB, Margarida GFC, Artur MM. 2014. Resistance in Capsicum spp. to anthracnose affected by different stages of fruit development during pre- and post-harvest. Tropical Plant Pathology 39:335–41.
Than PP, Jeewon R, Hyde KD, Pongsupasamit S, Mongkolporn O, Taylor PWJ. 2008. Characterization and pathogenicity of Colletotrichum species associated with anthracnose on chilli (Capsicum spp.) in Thailand. Plant Pathology 57:562–72.
Weryszko-Chmielewska E, Zenia M. 2012. Anatomical traits of sweet pepper (Capsicum annuum L.) fruit. Acta Agrobotanica 64:181–88.
Wharton PS, Schilder AC. 2008. Novel infection strategies of Colletotrichum acutatum on ripe blueberry fruit. Plant Pathology 57:122–34.
Wharton PS, Diéguez-Uribeondo J. 2004. The biology of Colletotrichum acutatum. Anales Del Jardin Bontanico de Madrid 61:3–22.
Yakoby N, Stanley F, Amos D, Noel TK, Dov P. 2000. Expression of pectate lyase from Colletotrichum gloeosporioides in C. magna promotes pathogenicity. Molecular Plant-Microbe Interactions 13:887–91.
Anand T, Ramanujam B, Thiruvengadam R, Gandhi K, Manikam R, Govindasamy S. 2008. Production of cell wall degrading enzymes and toxins by Colletotrichum capsici and Alternaria alternata causing fruit rot of chillies. J of Plant Protection Research 48:437–51.
Arroyo FT, Javier M, Gregorio G, Berta DS, Carmen B, Maria P, Cesar B, Fernando R. 2005. Ultrastructure of the early stages of Colletotrichum acutatum infection of strawberry tissue. Canadian J of Botany 83:491-500.
Bellincampi D, Felice C, Vincenzo L. 2014. Plant cell wall dynamics and wall-related susceptibility in planta-pathogen interactions. Frontiers in Plant Science 5:1–9.
Curry KJ, Maritza A, Jana BA, Barbara JS. 2002. Strawberry anthracnose:Histopathology of Colletotrichum acutatum and C. fragariae. Phytopathology 92:1055–63.
Desire MH, Fotso B, Muyang RF, Chiatoh TA, Ndoumou D. 2014. Enzymes and qualitative phytochemical screening of endophytic fungi isolated from Lantana camara Linn. J of Appl Bio and Biotech 2:1–6.
Devi PA, Prakasam V. 2014. Histopathology studies of snthracnose and powdery mildew diseases in chillies. World J of Biology and Biological Sci 2:98–101.
Elsenman HC, Arturo C. 2012. Synthesis and assembly of fungal melanin. Appl.Microbiol Biotechnol 93:931–40.
Gautam AK. 2014. The Genera Colletotrichum: An incitant of numerous new plant diseases in India. J on New Biological Reports 3:9–21.
Geetha S, Kokkaiah I, Sinthia G, Palanichamy M. 2016. Preliminary phytochemical screening of different solvent extracts and stem of Crataeva religiosa Hook Frost. Int J of Current Microbiol and Appl Sci 5:116–22.
González E, Turner BS, James CC. 2006. Clarification of the etiology of Glomerella leaf spot and bitter rot of apple caused by Colletotrichum spp. based on morphology and genetic, molecular, and pathogenicity tests. Phytopathology 96(9):982–92.
Guidarelli M, Carbone F, Mourgues F, Perrotta G, Rosati C, Bertolini P, Baraldi E. 2011. Colletotrichum acutatum interactions with unripe and ripe strawberry fruits and differential responses at histological and transcriptional levels. Plant Pathology 60:685–97.
Hakim A, Muhamad S, Widodo. 2014. Ketahanan penyakit antraknosa terhadap cabai lokal dan cabai introduksi. Bul. Agrohorti 2(1):31–36.
Liu GY, Victor N. 2009. Color me bad: Microbial pigments as virulence factors. Trends in Microbiology 17:406–13.
Muljowati JS, Loekas S, Laurentius HN. 2020. Production of pectinase enzymes by Colletotrichum acutatum Simmonds causing anthracnose in red chilli ( Capsicum annuum, L).P. 012036 in IOP Conference Series: Earth and Enviromnental Science, volume 593.
Pandey A, Pandey BK, Muthukumar M, Yadava LP, Chauhan UK. 2012. Histopathological study of infection process of Colletotrichum gloeosporioides Prnz and Sacc. on Mangifera indica L. Plant Pathology Journal 11:18–24.
Pardo EM, Grellet CF, Salazar SM, Castagnaro AP, Ricci JCD, Arias ME. 2012. Histopathology of the resistance to Colletotrichum gloeosporioides of wild strawberries and species related to commercial strawberry. Australian J of Crop Sci 6:1147–53.
Pusztahelyi T, Imre JH, István P. 2015. Secondary metabolites in fungus-plant interactions. Frontier in Plant Sci 6:1–23.
Saxena A, Richa R, Vijai KG, Harikesh BS. 2016. Chilli anthracnose: The epidemiology and management. Frontiers in Microbiology 7:1–18.
Silva SAM, Rosana R, Leandro SAGs, Cláudia PS, Cíntia SB, Margarida GFC, Artur MM. 2014. Resistance in Capsicum spp. to anthracnose affected by different stages of fruit development during pre- and post-harvest. Tropical Plant Pathology 39:335–41.
Than PP, Jeewon R, Hyde KD, Pongsupasamit S, Mongkolporn O, Taylor PWJ. 2008. Characterization and pathogenicity of Colletotrichum species associated with anthracnose on chilli (Capsicum spp.) in Thailand. Plant Pathology 57:562–72.
Weryszko-Chmielewska E, Zenia M. 2012. Anatomical traits of sweet pepper (Capsicum annuum L.) fruit. Acta Agrobotanica 64:181–88.
Wharton PS, Schilder AC. 2008. Novel infection strategies of Colletotrichum acutatum on ripe blueberry fruit. Plant Pathology 57:122–34.
Wharton PS, Diéguez-Uribeondo J. 2004. The biology of Colletotrichum acutatum. Anales Del Jardin Bontanico de Madrid 61:3–22.
Yakoby N, Stanley F, Amos D, Noel TK, Dov P. 2000. Expression of pectate lyase from Colletotrichum gloeosporioides in C. magna promotes pathogenicity. Molecular Plant-Microbe Interactions 13:887–91.
Most read articles by the same author(s)
- VINA MAULIDIA, LOEKAS SOESANTO, SYAMSUDDIN, KHAIRAN KHAIRAN, TAKAHIRO HAMAGUCHI, KOICHI HASEGAWA, RINA SRIWATI, Secondary metabolites produced by endophytic bacteria against the Root-Knot Nematode (Meloidogyne sp.) , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 11 (2020)
- Diego Mauricio Cano Reinoso, Loekas Soesanto, Kharisun Kharisun, Condro Wibowo, Review: Fruit collapse and heart rot disease in pineapple: Pathogen characterization, ultrastructure infections of plant and cell mechanism resistance , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 5 (2021)
- LOEKAS SOESANTO, ANNISA RAHMADDILA RIZKY HARTONO, ENDANG MUGIASTUTI, HERI WIDARTA, Seed-borne pathogenic fungi on some soybean varieties , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 9 (2020)
- LOEKAS SOESANTO, BISIRIL FATIHAH, ABDUL MANAN, ENDANG MUGIASTUTI, NUR PRIHATININGSIH , Organic control of Bemisia tabaci Genn. on Capsicum annuum with entomopathogenic fungi raw secondary metabolites , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 12 (2020)
- LOEKAS SOESANTO, ENDANG MUGIASTUTI, ABDUL MANAN, The use of alternative liquid media for propagation of pathogenic fungi and their effect on weeds , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 2 (2021)
- LOEKAS SOESANTO, RISMA ARSI SUSANTI, ENDANG MUGIASTUTI, ABDUL MANAN, MURTI WISNU RAGIL SASTYAWAN, JOKO MARYANTO, Remediation of chlorpyrifos-contaminated soils by crude secondary metabolites of Trichoderma harzianum T213 and its effect on maize growth , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 5 (2022)
- ENDANG MUGIASTUTI, ABDUL MANAN, LOEKAS SOESANTO, Biological control of maize downy mildew with the antagonistic bacterial consortium , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 9 (2023)
- LOEKAS SOESANTO, DHIMAS AULI SAPUTRA, MURTI WISNU RAGIL SASTYAWAN, ENDANG MUGIASTUTI, AGUS SUPRAPTO, RUTH FETI RAHAYUNIATI, Secondary metabolites of the granular form of Pseudomonas fluorescens P60 and its applications to control tomato bacterial wilt , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 4 (2023)
- WITIYASTI IMANINGSIH, NURAENI EKOWATI, SALAMIAH, NUNIEK INA RATNANINGTYAS, LOEKAS SOESANTO, Isolation of endophytic fungi from Hiyung chili peppers of local South Kalimantan (Indonesia) varieties and in vitro tolerance to acidic environment , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 7 (2023)