Molecular identification of coprophilous microfungi from Banyumas District, Central Java, Indonesia
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Abstract. Mumpuni A, Amurwanto A, Wahyono DJ. 2021. Molecular identification of coprophilous microfungi from Banyumas District, Central Java, Indonesia. Biodiversitas 22: 1550-1557. Coprophilous microfungi are a group of fungi that are ecologically interesting in relation to herbivores. These fungi play a predominant role in the decomposition of organic matter, in which the organic matter passes through a series of events involving mechanical degradation, as well as physical and biological processes. The role of coprophilous fungi as the main decomposers of the lignocellulosic material of herbivorous animal waste, which is widespread in nature, is very important. Previous research on the inventory and identification of coprophilous fungi in the Banyumas district has been limited to macroscopic genera, so the results have not been able to provide a comprehensive picture of the presence of coprophilous fungi in the region. Identification of the types of microscopic coprophilous fungi that live in herbivorous animal waste, such as lignocellulosic material, is necessary to understand the taxonomy of these fungi. This study aimed to investigate and identify microscopic coprophilous fungi obtained in the Banyumas district of Central Java, Indonesia. Based on the purposive random sampling method, the obtained fungi were analyzed using the molecular methods of DNA isolation, gene amplification, DNA sequencing and phylogenetic analysis of fungal cultures. The following species and genera were identified: Ceriporia lacerata, Trichosporon insectorum, Lentinus squarrosulus, Fusarium sp., Aspergillus sp., and Trichosporon sp.
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References
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Stackebrandt E, Goebel BM, 1994. Taxonomic Note: A Place for DNA-DNA Reassociation and 16S rRNA Sequence Analysis in the Present Species Definition in Bacteriology. International Journal of Systematic and Evolutionary Microbiology 44: 846–849.
Swofford DL. 2000. PAUP*: Phylogenetic Analysis Using Parsimony and Other Methods, Version 4.0b10, Sinauer Associates, Sunderland, MA (Mac version).
White TJ, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds. PCR Protocols: a Guide to Methods and Applications.
Wulandari R, Lotrakul P, Amirta R, Kim SW, Punnapayak H, Prasongsuk S. 2018. First record of Ceriporia inflata and Ceriporia lacerate (Phanerochaetaceae, Basidiomycota) from Indonesian tropical forest. Agriculture and Natural Resources 52: 412-418.
Zuber A, Kowalczyk M, Sekula A, Mleczko P, Kupiec T. 2011. Methods in species identification of hallucinogenic and other poisonous mushrooms in forensic investigation. Problems of Forensic Sciences 1036: 151-161.
Badotti F, de Oliveira FS, Garcia CF, Vaz ABM, Fonseca PLC, Nahum LA, Oliveira G, Góes-Neto A. 2017. Effectiveness of ITS and sub-regions as DNA barcode markers for the identification of Basidiomycota (Fungi). BMC Microbiology 17(42): 1-12
Bruce A, Johnson A, Lewis J, Raff M, Roberts K, Walter P. 2002. Molecular Biology of The Cell. 4th ed. New York, USA : Galand Science
Bruns TD, White TJ, Taylor JW. 1991. Fungal molecular systematics. Annu Rev col Syst. 22: 525-564.
Cen, YK, Lin JG, Wang YL, Wang JY, Liu ZQ, Zheng YG. 2020. The Gibberellin Producer Fusarium fujikuroi: Methods and Technologies in the Current Toolkit. Front. Bioeng. Biotechnol. 8: 232.
Hebert PDN, Stoeckle MY, Zemlak TS, Francis CM. 2004. Identification of Birds through DNA Barcodes. PLOS Biology 2(10): 1657-1663.
Hollingsworth PM. 2007. DNA barcoding: potential users. Genom Soc Pol. 3: 44-47.
Hu H, Ding S, Katayama Y, Kusumic A, Li SX, de Vries RP, Wang J, Yu XZ, Gu JD. 2013. Occurrence of Aspergillus allahabadii on sandstone at Bayon temple, Angkor Thom, Cambodia. International Biodeterioration & Biodegradation 76: 112-117
Krug JC, Benny GL, Keller HW. 2004. Coprophilous fungi. In: Mueller G.M., Bills G.F., Foster M.S., editors. Biodiversity of Fungi. Elsevier, Amsterdam.
Lee JS, Ko KS, Jung HK. 2000. Phylogenetic analysis of Xylaria based on nuclear ribosomal ITS1-5.8S-ITS2 sequences. FEMS Microbiology Letters 187: 89-93.
Lorliam W, Akaracharanya A, Suzuki M, Ohkuma M, Tanasupawati S. 2013. Diversity and fermentation products of xylose-utilizing yeasts isolated from buffalo feces in Thailand. Microbes Environ. 28 (3): 354–360.
Makhuvele R, Ncube I, van Rensburg ELJ, La Grange DC. 2017. Isolation of fungi from dung of wild herbivores for application in bioethanol production. Brazilian Journal of Microbiology 48: 646-655.
Masunga GS, Andresen O, Taylor JE, Dhillion SS. 2006. Elephant dung decomposition and coprophilous fungi in two habitats of semi-arid Botswana. Mycological Research 110(10): 1214-1226.
Melo RFR, Miller AN, Santiago ALCMA, Maia LC. 2014. The genera Ascobolus and Saccobolus (Ascobolaceae, Pezizales) in Brazil. Mycosphere 5(6): 790-804.
Melsasail L, Warouw VRC, Kamagi YEB. 2019. Analisis kandungan unsur hara pada kotoran sapi di daerah dataran tinggi dan dataran rendah. Cocos 2
Morais MH, Ramos AC, Matou N, Santous M, Oliveira EJ. 2000. Note: production of shitake mushroom (Lentinus edodes) on lignocellulosic residues. Food Sci. Technol. Int. 6:123–128.
Mumpuni A, Wahyono DJ. 2016. Inventarisasi Dan Identifikasi Secara Morfologis Jamur Koprofil Di Kawasan Wisata Pantai Parangtritis Yogyakarta. Makalah Seminar Nasional Biologi V UNNES Hilirisasi Hasil Penelitian Biologi dan pendidikan BiologiMelalui Akselerasi Inovasi Berwawawasan Konservasi. 29 Oktober 2016. Universitas Negeri Semarang.
Mumpuni A, Ekowati N, Wahyono D J. 2020. The existence of coprophilous macrofungi in Banyumas Central Java. Biodiversitas 21: 282-289.
Nilson RH, Kristiansson E, Ryberg M. 2008, Intraspecific ITS variability in the kingdom fungi as expressed in the international sequence databases and its implications for molecular species identification. Evolutionary Bioinformatics 4:193–201.
Obire O, Anyanwu EC, Okigbo RN. 2008. Saprophytic and crude oil degrading fungi from cow dung and poultry droppings as bioremediating agents. Journal of Agricultural Technology 4(2): 81-89.
Philippousis A, Zervakis G, Diamantopoulou P. 2001. Bioconversion of agricultural lignocellulosic wastes through cultivation of the edible mushrooms Agrocybe aegerita, Volvariella volvocea and Pleurotus spp. World J. Microbiol. Biotechnol. 17:191–200.
Sambrook J, Russel DW. 2001. Molecular Cloning: A Laboratory Manual (Third Edition). New York : Cold Spring Harbor LaboratoryPress.
Sinsabaugh RL, Benfield EF, Linkins AE, 1981. Cellulase activity associated with the decomposition of leaf litter in a woodland stream. Oikos 36: 184-190
Stackebrandt E, Goebel BM, 1994. Taxonomic Note: A Place for DNA-DNA Reassociation and 16S rRNA Sequence Analysis in the Present Species Definition in Bacteriology. International Journal of Systematic and Evolutionary Microbiology 44: 846–849.
Swofford DL. 2000. PAUP*: Phylogenetic Analysis Using Parsimony and Other Methods, Version 4.0b10, Sinauer Associates, Sunderland, MA (Mac version).
White TJ, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds. PCR Protocols: a Guide to Methods and Applications.
Wulandari R, Lotrakul P, Amirta R, Kim SW, Punnapayak H, Prasongsuk S. 2018. First record of Ceriporia inflata and Ceriporia lacerate (Phanerochaetaceae, Basidiomycota) from Indonesian tropical forest. Agriculture and Natural Resources 52: 412-418.
Zuber A, Kowalczyk M, Sekula A, Mleczko P, Kupiec T. 2011. Methods in species identification of hallucinogenic and other poisonous mushrooms in forensic investigation. Problems of Forensic Sciences 1036: 151-161.
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