CRISPR/Cas9-mediated genome editing in rice cv. IPB3S results in a semi-dwarf phenotypic mutant

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DOI https://doi.org/10.13057/biodiv/d220924
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Vol. 22 No. 9 (2021)
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Articles

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NURHAYATI
Program of Plant Breeding and Biotechnology, Graduate School, Institut Pertanian Bogor. Jl. Raya Dramaga, IPB Dramaga Campus, Bogor, West Java 16680, Indonesia
SINTHO WAHYUNING ARDIE
Plant Molecular Biotechnology Laboratory, Department of Agronomy and Horticulture, Faculty of Agriculture, Institut Pertanian Bogor. Jl. Meranti, IPB Dramaga Campus, Bogor 16680, West Java, Indonesia
TRI JOKO SANTOSO
Research and Development Center for Biotechnology and Genetic Resources. Jl. Tentara Pelajar No. 3A, Cimanggu, Bogor 16111, West Java, Indonesia
SUDARSONO ♥
Plant Molecular Biotechnology Laboratory, Department of Agronomy and Horticulture, Faculty of Agriculture, Institut Pertanian Bogor. Jl. Meranti, IPB Dramaga Campus, Bogor 16680, West Java, Indonesia

Abstract

Abstract. Nurhayati, Ardie SW, Santoso TJ, Sudarsono. 2021. CRISPR/Cas9-mediated genome editing in rice cv. IPB3S results in a semi-dwarf phenotypic mutant. Biodiversitas 22: 3792-3800. IPB3S is Indonesian lowland rice and high-yielding cultivar. However, plant height posture makes it prone to lodging which could reduce the yield. This study aimed to edit the GA20Ox2 gene by introducing CRISPR/Cas9 GA20Ox2 construct into IPB3S and developing the semidwarf rice mutants. Immature embryo explants of IPB3S were used for the transformation process mediated by EHA105 strain of Agrobacterium tumefaciens carrying pC1300-Cas9/GA20Ox2 and by changing the regeneration medium composition. PCR analysis showed that rice cv. IPB3S genetic transformation gained putative mutant T0 lines carrying the hpt gene (growth efficiency was 47.9%, while transformation efficiency was 19.3%). Using the developed regeneration medium, we have obtained 24 putative rice cv. IPB3S T0 mutant lines carrying hpt. The best medium for regenerating IPB3S was the A medium (regeneration efficiency 73.3%). IPB 8 and IPB 14 were potential to evaluate in the next generation. The shortest plant height for the T1 generation was observed in the IPB 8-3 mutant.

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References
Ashikari M, Sasaki A, Ueguchi-Tanaka M, Itoh H, Nishimura A, Datta S, Ishiyama K, Saito T, Kobayashi M, Khush GS. 2002. Loss-of-function of a rice gibberellin biosynthetic gene, GA20 oxidase (GA20ox-2), led to the rice “green revolution.” Breeding Science 52(2), 143–150. https://doi.org/10.1270/jsbbs.52.143
Chen S, Wang X, Zhang L, Lin S, Liu D, Wang Q, Cai S, El-Tanbouly R, Gan L, Wu H. 2016. Identification and characterization of tomato gibberellin 2-oxidases (GA2oxs) and effects of fruit-specific SlGA2ox1 overexpression on fruit and seed growth and development. Horticulture Research 3:1–9. https://doi.org/10.1038/hortres.2016.59
Chhun T, Aya K, Asano K, Yamamoto E, Morinaka Y, Watanabe M, Kitano H, Ashikari M, Matsuoka M, Ueguchi-Tanaka M. 2007. Gibberellin regulates pollen viability and pollen tube growth in rice. Plant Cell 19(12):3876–3888. https://doi.org/10.1105/tpc.107.054759
Cong L, Ran FA, Cox D, Lin S, Barreto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA. 2013. Multiplex Genome Engineering Using CRISPR/Cas Systems. Science Reports 339;819–824.
Davière JM, Achard P. 2013. Gibberellin signaling in plants. Development (Cambridge) 140(6):1147–1151. https://doi.org/10.1242/dev.087650
Davies PJ. 2004. Plant Hormones: Biosynthesis, Signal Transduction, Action. Journal of Chemical Information and Modeling Vol. 53. Kluwer Academic Publishers, Dordrecht. https://doi.org/10.1017/CBO9781107415324.004
Dello-Ioio R, Linhares FS, Scacchi E, Casamitjana-Martinez E, Heidstra R, Costantino P, Sabatini S. 2007. Cytokinins Determine Arabidopsis Root-Meristem Size by Controlling Cell Differentiation. Current Biology 17(8):678–682. https://doi.org/10.1016/j.cub.2007.02.047
Ge X, Chu Z, Lin Y, Wang S. 2006. A tissue culture system for different germplasms of indica rice. Plant Cell Reports 25(5):392–402. https://doi.org/10.1007/s00299-005-0100-7
Han Y, Teng K, Nawaz G, Feng X, Usman B, Wang X, Luo L, Zhao N, Liu Y, Li R. 2019. Generation of semi?dwarf rice (Oryza sativa L.) lines by CRISPR. Biotech 9:387. https://doi.org/10.1007/s1320 5-019-1919-x
Hiei Y, Komari T. 2006. Improved protocols for transformation of indica rice mediated by Agrobacterium tumefaciens. Plant Cell, Tissue and Organ Culture 85(3):271–283. https://doi.org/10.1007/s11240-005-9069-8
Hiei Y, Komari T. 2008. Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed. Nature Protocols 3(5):824–834. https://doi.org/10.1038/nprot.2008.46
Itoh H, Ueguchi-Tanaka M, Sakamoto T, Kayano T, Tanaka H, Ashikari M, Matsuoka M. 2002. Modification of rice plant height by suppressing the height-controlling gene D18 in rice. Breeding Science 52(3):215–218. https://doi.org/10.1270/jsbbs.52.215
Jiang W, Zhou H, Bi H, Fromm M, Yang B, Weeks DP. 2013. Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice. Nucleic Acids Research 41(20):1–12. https://doi.org/10.1093/nar/gkt780
Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. 2012. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337:816–821. https://doi.org/10.1126/science.1225829
Khush GS. 2005. What it will take to Feed 5.0 Billion Rice consumers in 2030. Plant Molecular Biology 59(1):1–6. https://doi.org/10.1007/s11103-005-2159-5
Lin YJ, Zhang Q. 2005. Optimising the tissue culture conditions for high efficiency transformation of indica rice. Plant Cell Reports 23(8):540–547. https://doi.org/10.1007/s00299-004-0843-6
Liu C, Zheng S, Gui J, Fu C, Yu H, Song D, Shen J, Qin P, Liu X, Han B. 2018. Shortened Basal Internodes Encodes a Gibberellin 2-Oxidase and Contributes to Lodging Resistance in Rice. Molecular Plant 11(2):288–299. https://doi.org/10.1016/j.molp.2017.12.004
Miki B, McHugh S. 2004. Selectable marker genes in transgenic plants: Applications, alternatives and biosafety. Journal of Biotechnology 107(3):193–232. https://doi.org/10.1016/j.jbiotec.2003.10.011
Ogawa Y, Mii M. 2007. Meropenem and moxalactam: Novel ?-lactam antibiotics for efficient Agrobacterium-mediated transformation. Plant Science 172(3):564–572. https://doi.org/10.1016/j.plantsci.2006.11.003
Ramesh M, Murugiah V, Gupta AK. 2009. Efficient in vitro plant regeneration via leaf base segments of indica rice (Oryza sativa L.). Indian Journal of Experimental Biology 47(1):68–74.
Sahoo RK, Tuteja N. 2012. Development of Agrobacterium-mediated transformation technology for mature seed-derived callus tissues of indica rice cultivar IR64. GM Crops and Food 3(2):123–128. https://doi.org/10.4161/gmcr.20032
Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, Swapan D, Ishiyama K, Saito T, Kobayashi M, Khush GS. 2002. A mutant gibberellin-synthesis gene in rice: New insight into the rice variant that helped to avert famine over thirty years ago. Nature 416:701–702. https://doi.org/10.1038/416701a
Slamet-Loedin IH, Chadha-Mohanty P, Torrizo L. 2014. Agrobacterium-Mediated Transformation: Rice Transformation Methods in, Vol. 1099, 97–115. Humana Press Springer, London. https://doi.org/10.1007/978-1-62703-715-0
Spielmeyer W, Ellis MH, Chandler PM. 2002. Semidwarf (sd-1), “green revolution” rice, contains a defective gibberellin 20-oxidase gene. Proceedings of the National Academy of Sciences of the United States of America 99(13):9043–9048. https://doi.org/10.1073/pnas.132266399
Thomas SG, Hedden P. 2018. Gibberellin Metabolism and Signal Transduction. Annual Plant Reviews Online 24(15):147–184. https://doi.org/10.1002/9781119312994.apr0247
Vennapusa AR, Vemanna RS, Reddy BH, Babitha KC, Kiranmai K, Nareshkumar A, Sudhakar C. 2015. An Efficient Callus Induction and Regeneration Protocol for a Drought Tolerant Rice Indica Genotype AC39020. Journal of Plant Sciences 3(5):248. https://doi.org/10.11648/j.jps.20150305.11
Wang E, Wang J, Zhu X, Hao W, Wang L, Li Q, Zhang L, He W, Lu B, Lin H. 2008. Control of rice grain-filling and yield by a gene with a potential signature of domestication. Nature Genetics 40(11):1370–1374. https://doi.org/10.1038/ng.22.
Wang Y, Li J. 2011. Branching in rice. Current Opinion in Plant Biology 14(1):94–99. https://doi.org/10.1016/j.pbi.2010.11.002
Wiedenheft B, Sternberg SH, Doudna JA. 2012. RNA-guided genetic silencing systems in bacteria and archaea. Nature 482(7385):331–338. https://doi.org/10.1038/nature10886
Xing Y, Zhang Q. 2010. Genetic and molecular bases of rice yield. Annual Review of Plant Biology 61:421–442. https://doi.org/10.1146/annurev-arplant-042809-112209
Zeng Y, Wen J, Zhao W, Wang Q, Huang W. 2020. Rational Improvement of Rice Yield and Cold Tolerance by Editing the Three Genes OsPIN5b, GS3, and OsMYB30 With the CRISPR–Cas9 System. Frontiers in Plant Science 10:1–13. https://doi.org/10.3389/fpls.2019.01663

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