Honey sugars profile of stingless bee Tetragonula laeviceps (Hymenoptera: Meliponinae)




Abstract. Agus A, Agussalim, Sahlan M, Sabir A. 2021. Honey sugars profile of stingless bee Tetragonula laeviceps (Hymenoptera: Meliponinae). Biodiversitas 22: 5205-5210. Honey was a functional food to improve human health, but irresponsible people used this circumstance to make fake honey. This study aimed to evaluate the profile of the sugar of stingless bee honey [Tetragonula laeviceps (Smith, 1857)] from different geographical origins in Indonesia. Honey, three samples were directly collected from three other sources for meliponiculture of T. laeviceps in Indonesia: Sleman, Klaten, and Gunungkidul. The honey sugars profile was analyzed: glucose, fructose, sucrose, reducing sugar, the sum of fructose and glucose, glucose to moisture ratio, fructose to glucose ratio, and honey pH. Glucose and fructose were analyzed by HPLC, sucrose by Luff Schoorl, reducing the sugar by Layne-Enyon, and pH by a pH meter. The current findings revealed that the geographical origins had a highly significant effect on glucose, fructose, the sum of fructose and glucose, glucose to moisture ratio, fructose to glucose ratio, and honey pH (P<0.01) and significant effect on reducing sugar (P<0.05), but not on sucrose content. Thus, it can be concluded that the origins were affecting the honey sugars profile and honey from Sleman has the highest sugars content, followed by honey from Klaten and the lower was honey from Gunungkidul.


Agus A, Agussalim, Nurliyani, Umami N, Budisatria IGS. 2019. Evaluation of antioxidant activity, phenolic, flavonoid and Vitamin C content of several honeys produced by the Indonesian stingless bee: Tetragonula laeviceps. Livest Res Rural Dev 31: 152.
Agussalim. 2020. Domestication and Meliponiculture of Stingless Bee Tetragonula laeviceps: Evaluation of Production Potency and Honey Quality as an Immunomodulator. [Dissertation]. Universitas Gadjah Mada, Yogyakarta. [Indonesian].
Agussalim, Agus A, Nurliyani, Umami N. 2019a. The sugar content profile of honey produced by the Indonesian Stingless bee, Tetragonula laeviceps, from different regions. Livest Res Rural Dev 31: 91.
Agussalim, Agus A, Nurliyani, Umami N. 2019b. Free amino acids profile of honey produced by the Indonesian stingless bee: Tetragonula laeviceps. In: The 8th International Seminar on Tropical Animal Production. pp. 149-152. Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, September 23-25, 2019.
Agussalim, Agus A, Nurliyani, Umami N, Budisatria IGS. 2019c. Physicochemical properties of honey produced by the Indonesian stingless bee: Tetragonula laeviceps. IOP Conf Ser Earth Environ Sci 387. https://doi.org/10.1088/1755-1315/387/1/012084.
Agussalim, Nurliyani, Umami N, Agus A. 2020. The honey and propolis production from Indonesian stingless bee: Tetragonula laeviceps. Livest Res Rural Dev 32: 121.
Agussalim, Umami N, Nurliyani, Agus A. 2021. The physicochemical composition of honey from Indonesian stingless bee (Tetragonula laeviceps). Biodiversitas 22: 3257-3263. https://doi.org/10.13057/biodiv/d220820.
Alvarez-Suarez JM, Giampieri F, Brenciani A, Mazzoni L, Gasparrini M, González-Paramás AM, Santos-Buelga C, Morroni G, Simoni S, Forbes-Hernández TY, Afrin S, Giovanetti E, Battino M. 2018. Apis mellifera vs Melipona beecheii Cuban polifloral honeys: A comparison based on their physicochemical parameters, chemical composition and biological properties. LWT - Food Sci Technol 87: 272-279. https://doi.org/10.1016/j.lwt.2017.08.079.
AOAC. 2005. Official Method of Association of Official Analytical Chemist. 18th Edition. Association of Official Analytical Chemist. Benjamin Franklin Station, Washington DC.
Biluca FC, Braghini F, Gonzaga LV, Costa ACO, Fett R. 2016. Physicochemical profiles, minerals and bioactive compounds of stingless bee honey (Meliponinae). J Food Compos Anal 50: 61-69. https://doi.org/10.1016/j.jfca.2016.05.007.
Bogdanov S, Lüllmann C, Martin P, von der Ohe W, Russmann H, Vorwohl G, Oddo LP, Sabatini AG, Marcazzan GL, Piro R, Flamini C, Morlot M, Lhéritier J, Borneck R, Marioleas P, Tsigouri A, Kerkvliet J, Ortiz A, Ivanov T, D’Arcy B, Mossel B, Vit P. 1999. Honey quality and international regulatory standards: Review by the international honey commission. Bee World 80: 61-69. https://doi.org/10.1080/0005772x.1999.11099428.
Chanchao C. 2013. Bioactivity of Honey and Propolis of Tetragonula laeviceps in Thailand. In: Vit P, Pedro S, Roubik D (eds.). Pot-Honey: A Legacy of Stingless Bees. Springer, New York, pp. 495-505.
Chuttong B, Chanbang Y, Sringarm K, Burgett M. 2016. Physicochemical profiles of stingless bee (Apidae: Meliponini) honey from South East Asia (Thailand). Food Chem 192: 149-155. https://doi.org/10.1016/j.foodchem.2015.06.089.
Codex Alimentarius. 2001. Draft revised standard for standard for honey (at step 10 of the Codex procedure). Alinorm 01: 19-26.
Da Silva PM, Gauche C, Gonzaga LV, Costa ACO, Fett R. 2016. Honey: Chemical composition, stability and authenticity. Food Chem 196: 309-323. https://doi.org/10.1016/j.foodchem.2015.09.051.
De La Fuente E, Ruiz-Matute AI, Valencia-Barrera RM, Sanz J, Martínez Castro I. 2011. Carbohydrate composition of Spanish unifloral honeys. Food Chem 129: 1483-1489. https://doi.org/10.1016/j.foodchem.2011.05.121.
Dobre I, Georgescu LA, Alexe P, Escuredo O, Seijo MC. 2012. Rheological behavior of different honey types from Romania. Food Res Int 49: 126-132. https://doi.org/10.1016/j.foodres.2012.08.009.
Erwan, Astuti M, Syamsuhaidi Muhsinin M, Agussalim. 2020. The effect of different beehives on the activity of foragers, honey pots number and honey production from stingless bee Tetragonula sp. Livest Res Rural Dev 32: 158.
Erwan, Suhardin, Syamsuhaidi, Purnamasari DK, Muhsinin M, Agussalim. 2021. Propolis mixture production and foragers daily activity of stingless bee Tetragonula sp . in bamboo and box hives. Livest Res Rural Dev 33: 82.
Escuredo O, Dobre I, Fernández-González M, Seijo MC. 2014. Contribution of botanical origin and sugar composition of honeys on the crystallization phenomenon. Food Chem 149: 84-90. https://doi.org/10.1016/j.foodchem.2013.10.097.
Escuredo O, Míguez M, Fernández-González M, Seijo MC. 2013. Nutritional value and antioxidant activity of honeys produced in a European Atlantic area. Food Chem 138: 851-856. https://doi.org/10.1016/j.foodchem.2012.11.015.
Fletcher MT, Hungerford NL, Webber D, Carpinelli de Jesus M, Zhang J, Stone ISJ, Blanchfield JT, Zawawi N. 2020. Stingless bee honey, a novel source of trehalulose: a biologically active disaccharide with health benefits. Sci Rep 10: 1-8. https://doi.org/10.1038/s41598-020-68940-0.
Guerrini A, Bruni R, Maietti S, Poli F, Rossi D, Paganetto G, Muzzoli M, Scalvenzi L, Sacchetti G. 2009. Ecuadorian stingless bee (Meliponinae) honey: A chemical and functional profile of an ancient health product. Food Chem 114: 1413-1420. https://doi.org/10.1016/j.foodchem.2008.11.023.
Juan-Borrás M, Domenech E, Hellebrandova M, Escriche I. 2014. Effect of country origin on physicochemical, sugar and volatile composition of acacia, sunflower and tilia honeys. Food Res Int 60: 86-94. https://doi.org/10.1016/j.foodres.2013.11.045.
Kahono S, Chantawannakul P, Engel MS. 2018. Social Bees and the Current Status of Beekeeping in Indonesia. In: Chantawannakul P, Williams G, Neumann P (eds.). Asian Beekeeping in the 21st Century. Springer, Singapore, pp. 287–306.
Kamal MA, Klein P. 2011. Determination of sugars in honey by liquid chromatography. Saudi J Biol Sci 18: 17-21. https://doi.org/10.1016/j.sjbs.2010.09.003.
Michener CD. 2013. The Meliponini. In: Patricia DV, Pedro SRM, Roubik DW (ed.). Pot - Honey: A Legacy of Stingless Bees. Springer, New York, pp. 3-17.
Nordin A, Sainik NQAV, Chowdhury SR, Saim AB, Idrus RBH. 2018. Physicochemical properties of stingless bee honey from around the globe: A comprehensive review. J Food Compos Anal 73: 91-102. https://doi.org/10.1016/j.jfca.2018.06.002.
Puscas A, Hosu A, Cimpoiu C. 2013. Application of a newly developed and validated high-performance thin-layer chromatographic method to control honey adulteration. J Chromatogr A 1272: 132-135. https://doi.org/10.1016/j.chroma.2012.11.064.
Ranneh Y, Ali F, Zarei M, Akim AM, Hamid HA, Khazaai H. 2018. Malaysian stingless bee and Tualang honeys: A comparative characterization of total antioxidant capacity and phenolic profile using liquid chromatography-mass spectrometry. LWT - Food Sci Technol 89: 1-9. https://doi.org/10.1016/j.lwt.2017.10.020.
Ribeiro RDOR, Mársico ET, Carneiro CDS, Monteiro MLG, Júnior CC, De Jesus EFO. 2014. Detection of honey adulteration of high fructose corn syrup by Low Field Nuclear Magnetic Resonance (LF 1H NMR). J Food Eng 135: 39-43. https://doi.org/10.1016/j.jfoodeng.2014.03.009.
Sabir A, Agus A, Sahlan M, Agussalim. 2021. The minerals content of honey from stingless bee Tetragonula laeviceps from different regions in Indonesia. Livest Res Rural Dev 33: 22.
SNI. 2018. Indonesian National Standard for Honey. Badan Standarisasi Nasional, Jakarta. [Indonesian]
Souza B, Roubik D, Barth O, Heard T, Enriquez E, Carvalho C, Villas-Boas J, Marchini L, Locatelli J, Persano-Oddo L, Almeida-Muradian L, Bogdanov S, Vit P. 2006. Composition of stingless bee honey: setting quality standards. Interciencia 31: 867-875.
Suntiparapop K, Prapaipong P, Chantawannakul P. 2012. Chemical and biological properties of honey from Thai stingless bee (Tetragonula leaviceps). J Apic Res 51: 45-52. https://doi.org/10.3896/IBRA.
Thrasyvoulou A, Tananaki C, Goras G, Karazafiris E, Dimou M, Liolios V, Kanelis D, Gounari S. 2018. Legislation of honey criteria and standards. J Apic Res 57: 88-96. https://doi.org/10.1080/00218839.2017.1411181.
Tornuk F, Karaman S, Ozturk I, Toker OS, Tastemur B, Sagdic O, Dogan M, Kayacier A. 2013. Quality characterization of artisanal and retail Turkish blossom honeys: Determination of physicochemical, microbiological, bioactive properties and aroma profile. Ind Crops Prod 46: 124-131. https://doi.org/10.1016/j.indcrop.2012.12.042.
Trianto M, Purwanto H. 2020. Morphological characteristics and morphometrics of stingless bees (Hymenoptera: Meliponini) in Yogyakarta, Indonesia. Biodiversitas 21: 2619-2628. https://doi.org/10.13057/biodiv/d210633.
Villacrés-Granda I, Coello D, Proaño A, Ballesteros I, Roubik DW, Jijón G, Granda-Albuja G, Granda-Albuja S, Abreu-Naranjo R, Maza F, Tejera E, González-Paramás AM, Bullón P, Alvarez-Suarez JM. 2021. Honey quality parameters, chemical composition and antimicrobial activity in twelve Ecuadorian stingless bees (Apidae: Apinae: Meliponini) tested against multiresistant human pathogens. LWT - Food Sci Technol 140: 110737. https://doi.org/10.1016/j.lwt.2020.110737.