Increasing the diversity of marigold (Tagetes sp.) by acute and chronic chemical induced mutation of EMS (Ethyl Methane Sulfonate)

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DEWI YANNY LENAWATY
DEWI SUKMA
MUHAMAD SYUKUR
DEWA NGURAH SUPRAPTA
WARAS NURCHOLIS
SYARIFAH IIS AISYAH

Abstract


Abstract. Lenawaty DY, Sukma D, Syukur M, Suprapta DN, Nurcholis W, Aisyah SI. 2022. Increasing the diversity of marigold (Tagetes sp.) by acute and chronic chemical induced mutation of EMS (Ethyl Methane Sulfonate)Biodiversitas 231399-1407. Increasing the diversity of marigold (Tagetes sp.) can be carried out through chemical induction mutations with Ethyl Methane Sulfonate (EMS).This study aims to obtain the LC50value, determine the sensitivity level of plants, and obtain plant diversity of Tagetes sp.with EMS both acutely and chronically. The genetic material used in this study was derived from T. erecta genotype MG04 and T. patula genotype MG21. The acute mutation technique was performed by soaking the seeds in EMS concentration for 4 hours under 10 different concentration levels (0%, 0.2%, 0.4%, 0.6%, 0.8%, 1.0%, 1.5%, 2.0%, and 3.0%). The chronic mutation technique was carried out by diluting the concentration of EMS LC501/10x, 1/100x, 1/1000x and immersion time of 6 hours, 24 hours and 48 hours. The study used a Completely Randomized Block Design. Observationswere made on the percentage of surviving plants, quantitative and qualitative characters. The data obtained were analyzed using Boxplot and ANOVA. The results showed that the LC50 value of T. erecta is 0.82% and LC50of T. patula is 1.87%, which means that the sensitivity level of T. erecta to EMS treatment is quite higher than T. patula. The acute application method shows a relatively low mutation rate and limited diversity of flower types. The chronic application method produced various flower shapes and whiter flower color in T. erectaand T. patula resulting in a redder flower color than the acute application method.


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References
Aisyah S. 2013. Mutasi Induksi. Di dalam: Syukur M, S. Sastrosumardjo. Sitogenetika Tanaman. Bogor (ID): IPB Press.
Aisyah SI, Marthin Y, Damanik MRM. 2015. Improving Performance of Coleus through Mutation Induction by Gamma Ray Irradiation. J Trop Crop Sci. 2(1):26–32. doi:10.29244/jtcs.2.1.26-32.
Bhusari A, Deshmukh M, Bhagat S. 2017. World journal of biology and biotechnology. World J Biol Biotechnol. 2(3):165–167.
Cveji? S, Afza R, Joci? S, Prodanovi? S, Mikli? V, Škori? D, Dragin S. 2011. Radiosensitivity of sunflower inbred lines to mutagenesis. Helia. 34(54):99–106. doi:10.2298/HEL1154099C.
Dewi IAP, Pharmawati M. 2018. Penggandaan Kromosom Marigold ( Tagetes erecta L .) dengan Perlakuan Kolkisin. J Ilm Biol Biosf. 35(3):153–157. doi:10.20884/1.mib.2018.35.3.773.
Fauziana M, Susandarini R. 2019. Species Diversity and Potential Use of Asteraceae in Tawangmangu, Karanganyar Regency, Central Java. J Trop Biodivers Biotechnol. 4(1):18. doi:10.22146/jtbb.36652.
Handayati W. 2013. Perkembangan Pemuliaan Mutasi Tanaman Hias di Indonesia Advancement of Mutation Breeding on Ornamental Plants in Indonesia. J Ilm Apl Isot dan Radiasi. 9(1):67–80.
Hase Y, Satoh K, Seito H, Oono Y. 2020. Genetic consequences of acute/chronic gamma and carbon ion irradiation of Arabidopsis thaliana. Front Plant Sci. 11 March:1–12. doi:10.3389/fpls.2020.00336.
Introduction G, The TO, Of E, Development THE, Harmonized OF, New OF, Of V. 2007. International union for the protection of new varieties of plants. Variety. 21:1–26.
Kangarasu S, Ganeshram S, Joel AJ. 2014. Determination of Lethal Dose For Gamma Rays and Ethyl Methane Sulphonate Induced Mutagenesis In Cassava (Manihot Esculenta Crantz.). Int J Sci Res. 3(1):3–6.
Keadtidumrongkul P, Chirarat N, Somran S. 2018. Determination of LD50 of Ethidium Bromide for Induction of Mutation in Marigolds. Sci Technol.(26):9.
Mangaiyarkarasi R, Girija M, Gnanamurthy S. 2014. Mutagenic effectiveness and efficiency of gamma rays and ethyl methane sulphonate in Catharanthus roseus. Int J Curr Microbiol Appl Sci. 3(5):881–889. https://www.researchgate.net/publication/293333872_Mutagenic_effectiveness_and_efficiency_of_gamma_rays_and_ethyl_methane_sulphonate_in_Catharanthus_roseus.
Pratiwi N made D, Pharmawati M, Astarini IA. 2013. Pengaruh Ethyl Methane Sulphonate ( EMS ) Terhadap Pertumbuhan dan Variasi Tanaman Marigold ( Tagetes sp . ). Agrotrop. 3(1):23–28.
Purente N, Chen B, Liu X, Zhou Y, He M. 2020. Effect of ethyl methanesulfonate on induced morphological variation in M3 generation of chrysanthemum indicum var. Aromaticum. HortScience. 55(7):1099–1104. doi:10.21273/HORTSCI15068-20.
Rahmah S. 2011. Induksi Keragaman Dua Varietas Krisan ( Dendranthema grandiflora Tzvelev ) dengan Etil Metana Sulfonat (EMS) Secara In Vitro.
Roychowdhury R, Tah J. 2011. Chemical mutagenic action on seed germination and related agro-metrical traits in M1 Dianthus generation. Curr Bot. 2(8):19–23. http://currentbotany.org/article/view/9242/4692.
Singh P, Krishna A, Kumar V, Krishna S, Singh K, Gupta M, Singh S. 2016. Chemistry and biology of industrial crop Tagetes Species: A review. J Essent Oil Res. 28(1):1–14. doi:10.1080/10412905.2015.1076740.
Suteja HN, Rostini N, Amien S. 2019. Pengaruh perlakuan ethyl methanesulphonate terhadap perkecambahan dan pertumbuhan kentang granola (biji). Kultivasi. 18(1):5–6. doi:10.24198/kultivasi.v18i1.19110.
Talebi A, Talebi A, Shahrokhifar B. 2012. Ethyl methane sulphonate (EMS) induced mutagenesis in malaysian rice (cv. MR219) for lethal dose determination. Am J Plant Sci. 3(12):1661–1665. doi:10.4236/ajps.2012.312202.
Yamaguchi H. 2018. Mutation breeding of ornamental plants using ion beams. Breed Sci. 68(1):71–78. doi:10.1270/jsbbs.17086.
Zulfahmi, David, Irfan M, Rosmaina, Ediyono S. 2018. Pengaruh Mutagen EMS Terhadap Perkecambahan dan Pertumbuhan Anakan Cabai Merah Keriting (Capsicum Annuum L.). Semin Nas Pembang Pertan Indones dalam Memperkuat Lumbung Pangan, Fundam Ekon dan Daya Saing Glob Univ Pembang Nas Veteran Yogyakarta, 16-17 Novemb 2018., siap terbit.

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