Possibility of glyphosate-resistant goosegrass (Eleusine indica) in citrus fields in Rejang Lebong Districts, Bengkulu, Indonesia

##plugins.themes.bootstrap3.article.main##

MARULAK SIMARMATA
REKO APRIANTONEDI
NANIK SETYOWATI
https://orcid.org/0000-0002-0768-9125
HESTI PUJIWATI

Abstract


Abstract. Simarmata M, Apriantonedi R, Setyowati N, Pujiwati H. 2023. Possibility of glyphosate-resistant goosegrass (Eleusine indica) in citrus fields in Rejang Lebong Districts, Bengkulu, IndonesiaBiodiversitas 243633-3638.Weed abundances dominated by goosegrass (Eleusine indica L.) are a major constraint in the citrus fields of Rejang Lebong District, Province of Bengkulu, Indonesia. The objectives of this research were to investigate weed control methods applied by citrus farmers, analyze the dominant weed, and study the possibility of glyphosate resistance in goosegrass. A survey was conducted by interviewing three citrus farmers regarding weed control measures, followed by weed assessment in 10 sampling plots of each location. Then, field trials were conducted to evaluate glyphosate efficacy on weeds in the three locations of citrus fields. The survey found that glyphosate has been applied for weed control up to 4 L ha-1( 1.92 kg a.i. ha-1 of isopropyl amine salt) 2 to 3 times a year, and the farmers found that some weeds were resistant to glyphosate. Weed assessment showed that goosegrass is a dominant species with a summed dominance ratio (SDR) of 67.1, 70.8, and 79.0% at each location, respectively. The field trials observed that the levels of survival weeds in the experimental plots treated with glyphosate at 0.5, 1.0, 2.0, 4.0, and 8.0 L ha-1were 94-95%, 83-85.3%, 70-73.3%, 20-29%, and 10-12.3%, respectively. Goosegrass was identified as the survivor's weeds. The glyphosate doses (LD50) required to eradicate 50% of the weed populations ranged between 2 and 4 L ha-1 at the three sites. This study proved that goosegrass in the citrus groves of Rejang Lebong District, Bengkulu, is resistant to glyphosate.


##plugins.themes.bootstrap3.article.details##

References
Baerson SR, Rodriguez DJ, Tran M, Feng Y, Biest NA, Dill GM. 2002. Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol 129:1265–1275. Doi:10.1104/pp.001560.
Beckie HJ. 2011. Herbicide-resistant weed management: focus on glyphosate. Pest Manag Sci 67(9):1037-1048. doi: 10.1002/ps.2195.
Chauhan BS and Johnson DE. 2008. Germination Ecology of Goosegrass (Eleusine indica): An Important Grass Weed of Rainfed Rice. Weed Sci 56:699-706. DOI: https://doi.org/10.1614/WS-08-048.1.
Chen J, Huang H, Wei S, Zhang C, Huang Z. 2015. Characterization of glyphosate-resistant goosegrass (Eleusine indica) populations in China. J Integr Agric 14:919–925. https://doi.org/10.1016/S2095-3119(14)60910-2.
Chen J, Huang H, Wei S, Cui H, Li X, Zhang C. 2020. Glyphosate resistance in Eleusine indica: EPSPS overexpression and P106A mutation evolved in the same individuals. Pestic Biochem Physiol 164:203–208. https://doi.org/10.1016/j.pestbp.2020.01.014.
Chen J, Huang H, Wei S, Huang Z, Wang H, Zhao D, Zhang C. 2017. Characterization of Eleusine indica with gene mutation or amplification in EPSPS to glyphosate. Pestic Biochem Physiol 143:201–206. https://doi.org/10.1016/j.pestbp.2017.09.012.
Fernández-Moreno PT, Travlos I, Brants I. 2017. Different levels of glyphosate-resistant Lolium rigidum L. among major crops in southern Spain and France. Sci Rep 7:13116. https://doi.org/10.1038/s41598-017-13384-2
Gaines TA, Duke SO, Morran S, Rigon CAG, Tranel PJ, Küpper A, and Dayan FE. 2020. Mechanisms of evolved herbicide resistance. J Biol Chem 295(30):10307-10330. DOI: 10.1074/jbc.REV120.013572.
Heap I. 2014. Herbicide Resistant Weeds. Integrated Pest Manag 3:281–301. doi:10.1007/978-94-007-7796-5_12
Heap I. 2022. International Survey of Herbicide Resistant Weed. http://wwwscience.org. (Retrieved on September 8, 2022).
Heap I, and Duke SO. 2018. Overview of glyphosate-resistant weeds worldwide. Pest Manag Sci 74:1040–1049. DOI: 10.1002/ps.4760.
Jalaludin A, Yu Q, Powles SB. 2015. Multiple resistance across glufosinate, glyphosate, paraquat and ACCase-inhibiting herbicides in an Eleusine indica population. Weed Res 55:82–89. DOI:10.1111/WRE.12118.
Julianto. 2015. Hunting Gerga Oranges to Bengkulu. Tabloid Sinar Tani. https://tabloidsinartani.com/detail/indeks/klinik-teknologi/2300-berburu-jeruk-gerga-hingga-ke-bengkulu- (Retrieved on October 12, 2022 (Indonesian).
Lee LJ and Ngim J. 2000. A first report of glyphosate-resistant goosegrass (Eleusine indica L. (Gaertn) in Malaysia. Pest Manag Sci 56(4):336–339. DOI: 1002/(SICI)1526-4998(200004)56:4<336::AID-PS123>3.0.CO;2-8.
Loddo D, Imperatore G, Milani A, Panozzo S, Farinati S, Sattin M and Zanin G. 2020. First Report of Glyphosate-Resistant Biotype of Eleusine indica (L.) Gaertn. in Europe. Agronomy 10(1692):11 pp. doi:10.3390/agronomy10111692.
Manik, SE. 2019. Resistance Test of Eleusine indica against Glyphosate Herbicide (Indonesian). AGRILAND Jurnal Ilmu Pertanian, 7(1):33-38. (Indonesian).
Pavlovic D, Reinhardt C, Elezovi? I and Vrbni?anin S. 2011, Identification of Glyphosate Resistance in Lolium rigidum Gaudin. Pestic Phytomed, 26(4):393-399. DOI: 10.2298/PIF1104393P.
Peterson MA, Collavo A, Ovejero R, Shivraind V and Walshe MJ. 2018. The challenge of herbicide resistance around the world: a current summary. Pest Manag Sci 74(10):2246-2259. DOI 10.1002/ps.4821.
Powles SB, Debrah FLC, James JD, Preston C. 1998. Evolved Resistance to Glyphosate in rigid ryegrass (Lolium rigidum) in Australia. Weed Sci 46:604–607. 10.1017/S0043174500091165.
Preston C, Wakelin AM, Dolman FC, Bostamam Y and Boutsalis P. 2009. A decade of glyphosate-resistant Lolium around the world: mechanisms, genes, fitness, and agronomic management. Weed Sci 57:435–441. DOI: 10.1614/WS-08-181.1.
Purba E and Sipayung R. 2021. Confirmation and control of glyphosate-resistant biotype of goosegrass (Eleusine indica L.). In Sumatran oil palm. JSSAS 21(7):318-323. DOI: 10.1016/j.jssas.2021.10.010.
Ouyang C, Liu W, Chen S, Zhao H, Chen X, Jin X, Li X, Wu Y, Zeng X, Huang P, He X, An B. 2021. The Naturally Evolved EPSPS from Goosegrass Confers High Glyphosate Resistance to Rice. Front Plant Sci 12(756116):12 pp. doi: 10.3389/fpls.2021.756116.
Rambe SSMR, Supriyanto A, Afrizon, Calista I, Ifanti L, Dinata K, Honorita B dan Robiyanto. 2012. Final Report on the Technology of Flowering and Fruiting of Gerga Citrus in Lebong. Bengkulu Agricultural Technology Study Center. Center for the Assessment and Development of Agricultural Technology. Agricultural Research and Development Agency. Ministry of Agriculture. (Indonesian).
Rosalina Y, Susanti L, Karo N. 2017. Study of Pectin Extraction from Waste of Rimau Gerga Lebong Oranges (RGL Oranges) and Kalamansi Oranges. Agrointek, 12(2):68-74. DOI: 10.21107/agrointek.v11i2.3174.
Saidi N, Kadir J and Hong LW. 2017. Genetic Diversity and Morphological Variations of Goosegrass [Eleusine indica (L.) Gaertn] Ecotypes in Malaysia. Weed Turf Sci 5(3):144~154. http://dx.doi.org/10.5660/WTS.2016.5.3.144.
Sammons RD and Gaines TA. 2017. Glyphosate resistance: state of knowledge. Pest Manag Sci 70(9):1367-77. doi: 10.1002/ps.3743.
Simarmata M and Penner D. 2008. The Basis for Glyphosate Resistance in Rigid Ryegrass (Lolium rigidum) from California. Weed Sci 56:181-188. http://dx.doi.org/10.1614/WS-07-057.1.
Simarmata M, Sitanggang CD and Djamilah D. 2015. Shifting weed compositions and biomass production in sweet corn field treated with organic composts and chemical weed controls. AGRIVITA J Agric Sci 37:226-236. DOI: http://doi.org/10.17503/agrivita.v37i3.582.
Simarmata M, Kaufmann JE and Penner D. 2003. Potential Basis of Glyphosate Resistance in California Rigid Ryegrass (Lolium rigidum). Weed Sci 51:678-682. DOI:10.1614/P2002-124.
Simarmata M, Taufik M and Peranginangin ZZA. 2017. Efficacy of paraquat and glyphosate applied in water solvents from different sources to control weeds in oil palm plantation. ARPN J Agric Biol Sci 12:58-64.
Singh UP, Kamboj A, Sharma M. 2020. Herbicide Resistance in Weed and Its Management-A Review. IJARESM 8(12): 123-223.
Suryani S, Rambe M and IvantiL. 2015. Effect of Fertilization and Pruning on the Quality of Gerga Lebong Citrus Fruits. https://adoc.pub/pengaruh-pemupukan-dan-pemangkasan-terhadap-kualitas-buah-je.html. (Retrieved on July 15, 2022).
Takano HK, Mendes RR, Scoz LB, Lopez-Ovejero RF, Constantin J, Gaines TA, Westra P, Dayan FE and Oliveira RS. 2019. Proline-106 EPSPS mutation imparting glyphosate resistance in goosegrass (Eleusine indica) Emerges in South America. Weed Sci 67:48–56. https://doi.org/10.1017/wsc.2018.71.
Takano HK, deOliveira Júnior RS, Konstantin J, Braz GBP and Gheno EA. 2017. Goosegrass resistant to glyphosate in Brazil. Planta Daninha 35: 10 pp. DOI:10.1590/s0100-83582017350100013.
Tampubolon K, Purba P, Basyuni M and Hanafiah DS. 2019. Glyphosate resistance of Eleusine indica populations from North Sumatra, Indonesia. Biodiversitas 20(7):1910–1916. https://doi.org/10.13057/biodiv/d200717
Yanniccari M, Istilart C, Giménez DO and Castro AM. 2012. Glyphosate resistance in perennial ryegrass (Lolium perenne L.) from Argentina. Crop Prot, 32, 12–16. doi: 10.1016/j.cropro.2011.09.021.
Zimdahl RL. 2018. Properties and Uses of Herbicides in: Fundamentals of Weed Science, fifth ed., Elsevier Inc., pp. 463–499. https://doi.org/10.1016/B978-0-12-811143-7.00016-0.