Phylogenetic analysis of Dengue virus and insect-specific Flavivirus in Aedes aegypti from Gresik, Indonesia 2019

##plugins.themes.bootstrap3.article.sidebar##

PDF
DOI https://doi.org/10.13057/biodiv/d250602
Issue
Vol. 25 No. 6 (2024)
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

##plugins.themes.bootstrap3.article.main##

RASYID NOOR HAKIM
Graduate Program, Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia
MAMIK DAMAYANTI
Akademi Farmasi Mitra Sehat Mandiri Sidoarjo. Jl. Ki Hajar Dewantara No. 200, Sidoarjo 61262, East Java, Indonesia
SHIFA FAUZIYAH
Akademi Analisis Kesehatan Delima Husada. Jl. Arif Rahman Hakim No. 2B, Gresik 61111, East Java, Indonesia
TEGUH HARI SUCIPTO ♥
Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia

Abstract

Abstract. Hakim RN, Damayanti M, Fauziyah S, Sucipto TH. 2024. Phylogenetic analysis of Dengue virus and insect-specific Flavivirus in Aedes aegypti from Gresik, Indonesia 2019. Biodiversitas 25: 2340-2347. The spread of several Flaviviruses, such as Dengue virus and insect-specific Flavivirus, is periodically detected in Indonesia, including Surabaya, which is an endemic area. Gresik, as one of Surabaya's satellite cities, has high traffic mobility between regions, increasing the possibility of viral transmission. In this study, we identified Flaviviruses from Aedes aegypti mosquito isolates from Gresik collected in 2019. We also revealed the possibility of virus transmission from Surabaya to Gresik. The mosquito samples were collected using mosquito trap method. The Flavivirus Partial Nonstructural Protein 5 (NS5) -encoding gene region was isolated via the nested reverse transcription-polymerase chain reaction method. Sequence alignment and phylogenetic analysis were carried out to determine the kinship of Gresik strain virus isolates with the GenBank database. The results revealed that six out of 29 isolated samples (Sample from Duduk Sampeyan (47 and 43), Mengare (212 and 31), Kedamean (58), and Manyar (38)) exhibited a 220 bp DNA band, which corresponds to the NS5 gene. Further analysis using basic local alignment search tool - nucleotide and phylogenetic methods revealed that two sample sequences (38 and 212) are closely related to DENV-1 genotype 1, while the other four (31, 43, 47, and 58) are associated with Insect-Spesific Flavivirus (ISF). The similarity in NS5 partial sequence and genotype classifications of the dengue virus suggests the potential transmission of the virus from Surabaya to Gresik. We hypothesize that this phenomenon is closely linked to the population mobility between the two areas.

##plugins.themes.bootstrap3.article.details##

References
Agboli E, Leggewie M, Altinli M, Schnettler E. 2019. Mosquito-specific viruses—transmission and interaction. Viruses 11 (873): 1-26. DOI: 10.3390/v11090873.
Badan Pusat Statistik Kabupaten Gresik. 2023. Kabupaten Gresik dalam angka 2023. Badan Pusat Statistik Kabupaten Gresik, Gresik. www.gresikkab.bps.go.id.
Blitvich BJ, & Firth AE. 2015. Insect-specific flaviviruses: A systematic review of their discovery, host range, mode of transmission, superinfection exclusion potential and genomic organization. Viruses 7 (4): 1927-1959. DOI: 10.3390/v7041927.
Bolling BG, Vasilakis N, Guzman H, Widen SG, Wood TG, Popov VL, Thangamani S, Tesh RB. 2015. Insect-specific viruses detected in laboratory mosquito colonies and their potential implications for experiments evaluating arbovirus vector competence. Am J Trop Med Hyg 92 (2): 422–428. DOI: 10.4269/ajtmh.14-0330.
Damayanti PAA, Astawa INM, Adi AAAM, Sudarmaja IM, Swastika IK, Laksemi DAAS, Diarthini NLPE. 2021. Mosquito-specific viruses (family Flaviviridae, genus Flavivirus) diisolasi pada nyamuk Anopheles vagus di Bali. Jurnal Veteriner 22 (2): 189–197. DOI: 10.19087/jveteriner.2021.22.2.189.
Datu AM, Natzir R, Yustisia I, Wahid I, Soraya GV, Kadir S. 2023. Molecular detection and analysis of Dengue virus genetic diversity in North Sulawesi, Indonesia during 2022. Biodiversitas 24 (6): 3407–3413. DOI: 10.13057/biodiv/d240636
Dinas Kesehatan Kabupaten Gresik. 2019. Laporan Kinerja Instansi Pemerintah: Dinas Kesehatan Kabupaten Gresik Tahun 2018. Dinas Kesehatan Kabupaten Gresik, Gresik. www.gresikkab.go.id.
Dinas Kesehatan Provinsi Jawa Timur. 2022. Profil Kesehatan Provinsi Jawa Timur Tahun 2022. Dinas Kesehatan Provinsi Jawa Timur. Surabaya. www.dinkes.jatimprov.go.id.
Goncalvez AP, Escalante AA, Pujol FH, Ludert JE, Tovar D, Salas RA, Liprandi F. 2002. Diversity and evolution of the envelope gene of Dengue virus type 1. Virol 303 (1): 110–119. DOI: 10.1006/viro.2002.1686
Guzman H, Contreras-Gutierrez MA, Travassos da Rosa APA, Nunes MRT, Cardoso JF, Popov VL, Young KI, Savit C, Wood TG, Widen SG, Watts DM, Hanley KA, Perera D, Fish D, Vasilakis N, Tesh RB. 2018. Characterization of three new Insect-specific flaviviruses: Their relationship to the Mosquito-borne flavivirus pathogens. Am J Trop Med Hyg 98 (2): 410–419. DOI: 10.4269/ajtmh.17-0350.
Hamel R, Surasombatpattana P, Wichit S, Dauvé A, Donato C, Pompon J, Vijaykrishna D, Liegeois F, Vargas R, Luplertlop N, Missé D. 2019. Phylogenetic analysis revealed the co-circulation of four Dengue virus serotypes in Southern Thailand. PLoS ONE 14 (8): 1–16. DOI: 10.1371/journal.pone.0221179.
Herijanto W. 2018. Sustainable Urban Transport Index Surabaya, Indonesia. ESCAP, Bangkok. www.unescap.org
Hoshino K, Isawa H, Tsuda Y, Sawabe K, Kobayashi M. 2009. Isolation and characterization of a new Insect flavivirus from Aedes albopictus and Aedes flavopictus mosquitoes in Japan. Virol 391 (1): 119–129. DOI: 10.1016/j.virol.2009.06.025.
Hoshino K, Takahashi-Nakaguchi A, Isawa H, Sasaki T, Higa Y, Kasai S, Tsuda Y, Sawabe K, Kobayashi M. 2012. Entomological surveillance for Flaviviruses at migratory bird stopover sites in Hokkaido, Japan, and a new Insect flavivirus detected in Aedes galloisi (Diptera: Culicidae). J Med Entomol 49 (1): 175–182. DOI: 10.1603/ME11123.
Islam MT, Quispe C, Herrera-Bravo J, Sarkar C, Sharma R, Garg N, Fredes LI, Martorell M, Alshehri MM, Sharifi-Rad J, Da?tan SD, Calina D, Alsafi R, Alghamdi S, Batiha GES, Cruz-Martins N. 2021. Production, transmission, pathogenesis, and control of dengue virus: a literature-based undivided perspective. BioMed Res Int 2021 (4224816): 1-23. DOI: 10.1155/2021/4224816.
Karyanti MR, Uiterwaal CSPM, Kusriastuti R, Hadinegoro SR, Rovers MM, Heesterbeek H, Hoes AW, Bruijning-Verhagen P. 2014. The changing incidence of Dengue haemorrhagic fever in Indonesia: A 45-year registry-based analysis. BMC Infect Dis 14 (412): 1–7. DOI: 10.1186/1471-2334-14-412.
Kementrian Kesehatan Republik Indonesia. 2022. Profil Kesehatan Indonesia Tahun 2022. Kementrian Kesehatan Republik Indonesia, Jakarta. www.kemkes.go.id
Kotaki T, Yamanaka A, Mulyatno KC, Churrotin S, Sucipto TH, Labiqah A, Ahwanah NLF, Soegijanto S, Kameoka M, Konishi E. 2016. Divergence of the Dengue virus type 2 Cosmopolitan genotype associated with two predominant serotype shifts between 1 and 2 in Surabaya, Indonesia, 2008-2014. Infect Genet Evol 37 (2016): 88–93. DOI: 10.1016/j.meegid.2015.11.002.
Lestari CSW, Yohan B, Yunita A, Meutiawati F, Hayati RF, Trimarsanto H, Sasmono RT. 2017. Phylogenetic and evolutionary analyses of Dengue viruses isolated in Jakarta, Indonesia. Virus Genes 53 (6): 778–788. DOI: 10.1007/s11262-017-1474-7.
Martin E, Chirivella M, Co JKG, Santiago GA, Gubler DJ, Muñoz-Jordán JL, Bennett SN. 2016. Insights into the molecular evolution of Dengue virus type 4 in Puerto Rico over two decades of emergence. Virus Res 213 (2016): 23–31. DOI: 10.1016/j.virusres.2015.11.009.
Mulyatno KC, Kotaki T, Yotopranoto S, Rohmah EA, Churotin S, Sucipto TH, Amarullah IH, Wardhani P, Soegijanto S, Kameoka M. 2018. Detection and serotyping of Dengue viruses in Aedes aegypti and Aedes albopictus (Diptera: Culicidae) collected in Surabaya, Indonesia from 2008 to 2015. Jpn J Infect Dis 71 (1): 58–61. DOI: 10.7883/yoken.JJID.2017.117.
Murugesan A, & Manoharan M. 2019. Dengue virus. In Ennaji MM (eds). Emerging and Reemerging Viral Pathogens: Volume 1: Fundamental and Basic Virology Aspects of Human, Animal and Plant Pathogens. Elsevier, Amsterdam.
Peinado SA, Aliota MT, Blitvich BJ, & Bartholomay LC. 2022. Biology and transmission dynamics of Aedes flavivirus. J Med Entomol 59 (2): 659–666. DOI: 10.1093/jme/tjab197.
Regato M, Recarey R, Moratorio G, de Mora D, Garcia-Aguirre L, Gónzalez M, Mosquera C, Alava A, Fajardo A, Alvarez M, D' Andrea L, Dubra A, Martínez M, Khan B, Cristina J. 008. Phylogenetic analysis of the NS5 gene of Dengue viruses isolated in Ecuador. Virus Res 132 (2008): 197–200. DOI: 10.1016/j.virusres.2007.10.012.
Salih RRM, Karrar AEE, Aradaib IEET. 2020. Nested Polymerase Chain Reaction for Identification of Mitochondrial Cytochrome B Gene of Gazella dorcas. Am J Biomed Sci Res 7 (1): 16-18. DOI: 10.34297/AJBSR.2020.07.001105.
Sasmono RT, Wahid I, Trimarsanto H, Yohan B, Wahyuni S, Hertanto M, Yusuf I, Mubin H, Ganda IJ, Latief R, Bifani PJ, Shi PY, Schreiber MJ. 2015. Genomic analysis and growth characteristic of Dengue viruses from Makassar, Indonesia. Infect Genet Evol 32 (2015): 165–177. DOI: 10.1016/j.meegid.2015.03.006.
Scaramozzino N, Crance JM, Jouan A, DeBriel DA, Stoll F, Garin D. 2001. Comparison of Flavivirus universal primer pairs and development of a rapid, highly sensitive heminested reverse trancription-PCR assay for detection of Flaviviruses targeted to a conserved region of the NS5 gene sequences. J Clin Microbiol 39 (5): 1922–1927. DOI: 10.1128/JCM.39.5.1922-1927.2001.
Singh S, Gupta BP, Manakkadan A, Manandhar KD, Sreekumar E. 2015. Phylogenetic study reveals co-circulation of Asian II and Cosmopolitan genotypes of Dengue virus serotype 2 in Nepal during 2013. Infect Genet Evol 34 (2015): 402–409. DOI: 10.1016/j.meegid.2015.07.006.
Soegijanto S, Churrotin S, Sucipto TH, Aryanti N. 2022. Dengue virus infections in the first six months of 2018 in Surabaya, Indonesia. Gazz Med Ital Arch S 181 (4): 187-190. DOI: 10.23736/S0393-3660.20.04350-8.
Stollar V, Thomas VL. 1975. An agent in the Aedes aegypti cell line (Peleg) which causes fusion of Aedes albopictus cells. Virol 64 (2): 367–377. DOI: 10.1016/0042-6822(75)90113-0
Sumarmo. 1987. Dengue haemorrhagic fever in Indonesia. Southeast Asian J Trop Med Public Health 18 (3): 269-274.
Supriyono, Kuwata R, Torii S, Shimoda H, Ishijima K, Yonemitsu K, Minami S, Kuroda Y, Tatemoto K, Tran NTB, Takano, A, Omatsu T, Mizutani T, Itokawa K, Isawa H, Sawabe K, Takasaki T, Yuliani DM, Abiyoga D, Hadi UK, Setiyono A, Hondo E, Agungpriyono, S, Maeda K. 2020. Mosquito-borne viruses, Insect-specific flaviviruses (Family Flaviviridae, genus Flavivirus), Banna virus (family Reoviridae, genus Seadornavirus), Bogor virus (unassigned member of family Permutotetraviridae), and Alphamesoni viruses 2 and 3 (family Meson). J Vet Med Sci 82 (7): 1030–1041. DOI: 10.1292/JVMS.20-0261.
Wang E, Ni H, Xu R, Barrett ADT, Watowich SJ, Gubler DJ & Weaver SC. 2000. Evolutionary relationships of endemic/epidemic and sylvatic dengue viruses. Virol 74 (7): 3227-3234.
Wardhani P, Aryati A, Yohan B, Trimarsanto H, Setianingsih TY, Puspitasari D, Arfijanto MV, Bramantono B, Suharto S, Sasmono RT. 2017. Clinical and virological characteristics of dengue in Surabaya, Indonesia. PLoS ONE 12 (6): 1-21. DOI: 10.1371/journal.pone.0178443.
Yohan B, Dhenni R, Hayati RF, Yudhaputri FA, Denis D, Pamai YWB, Afida AM, Hutagalung IA, Haryanto S, Trimarsanto H, Myint KSA, Sasmono RT. 2018. Whole genome sequencing of Indonesian Dengue virus isolates using next-generation sequencing. Indones J Biotechnol 23 (2): 74–83. DOI: 10.22146/ijbiotech.38788.

Most read articles by the same author(s)