Peat-derived Streptomyces spp. isolated from edamame rhizosphere with plant growth-promoting properties
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Abstract. Erdiandini I, Tjahjoleksono A, Astuti RI, Husen E, Wahyudi AT. 2025. Peat-derived Streptomyces spp. isolated from edamame rhizosphere with plant growth-promoting properties. Biodiversitas 26: 326-334. Peatlands, which are known for their high organic matter content, are common habitats for actinomycetes. These microorganisms have been recognized for their potential as plant growth promoters. However, there have been limited reports on peat-derived actinomycetes with plant growth-promoting properties, especially in edamame-cultivated peatlands. This study aimed to isolate and investigate plant growth-promoting actinomycetes in the rhizosphere of edamame-cultivated peatlands. The results showed that a total of 46 strains were isolated from the edamame rhizosphere during the anthesis and reproductive phase. Importantly, 36 of these strains were found to be biologically safe, as showed by the negative hemolysis and hypersensitivity test. All 36 strains produced indole-3-acetic acid in the range of 2.42 to 50.07 µg/mL. Based on the in vivo plant growth-promoting activity assay, strains RT34, AR26, AR39, and BT59 promoted the highest primary root growth of edamame sprouts. Interestingly, the acetylene reduction assay revealed that only RT34 and AR39 strains exhibited nitrogenase activity as high as that of Azotobacter sp. as a positive control. The nitrogenase activity of these strains was up to 27.42 nmol C2H4 h-1tube-1 and 28.10 nmol C2H4 h-1tube-1. Furthermore, quantitative phosphate solubilization assay showed phosphate solubilization up to 527.76 µg/mL in the range of 258.31 to 527.76 µg/mL. Based on 16S rRNA sequencing, these strains were closely related to the genus Streptomyces. The finding of present results strongly indicates that these Streptomyces strains have plant growth-promoting properties and can be proposed as biostimulants to enhance edamame growth in peatlands.
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References
Abdelgawad H, Abuelsoud W, Madany MMY, Selim S, Zinta G, Mousa ASM, Hozzein WN. 2020. Actinomycetes enrich soil rhizosphere and improve seed quality as well as productivity of legumes by boosting nitrogen availability and metabolism. Biomolecules 10 (12): 1675. DOI: 10.3390/BIOM10121675.
Abebe TG, Tamtam MR, Abebe AA, Abtemariam KA, Shigut TG, Dejen YA, Haile EG. 2022. Growing use and impacts of chemical fertilizers and assessing alternative organic fertilizer sources in Ethiopia. Appl Environ Soil Sci 2022: 4738416. DOI: 10.1155/2022/4738416.
Adedeji AA, Häggblom MM, Babalola OO. 2020. Sustainable agriculture in Africa: Plant Growth-Promoting Rhizobacteria (PGPR) to the rescue. Sci Afr 9: e00492. DOI: 10.1016/j.sciaf.2020.e00492.
Akshatha SJ, Kalyani M. 2022. Evaluation of mangrove soil Streptomyces spp. exhibiting culture and biochemical variation for determination of antibacterial activity. J Pure Appl Microbiol 16 (4): 2458-2476. DOI: 10.22207/JPAM.16.4.06.
Aly MM, Bahamdain LA, Aba SA. 2019. Unexplored extreme habitats as sources of novel and rare Actinomycetes with enzyme and antimicrobial activities. IOSR-J Pharm Biol Sci 14: 45-54. DOI: 10.9790/3008-1406034554.
Amri MF, Husen E, Tjahjoleksono A, Wahyudi AT. 2022. Alkaline phosphatase activity of plant growth-promoting Actinomycetes and their genetic diversity based on the phoD gene. HAYATI J Biosci 29: 360-369. DOI: 10.4308/HJB.29.3.360-369.
Anda M, Ritung S, Suryani E, Sukarman, Hikmat M, Yatno E, Mulyani A, Subandiono RE, Suratman, Husnain. 2021. Revisiting tropical peatlands in Indonesia: Semi-detailed mapping, extent and depth distribution assessment. Geoderma 402: 115235. DOI: 10.1016/j.geoderma.2021.115235.
Bargaz A, Elhaissoufi W, Khourchi S, Benmrid B, Borden KA, Rchiad Z. 2021. Benefits of phosphate solubilizing bacteria on belowground crop performance for improved crop acquisition of phosphorus. Microbiol Res 252: 126842. DOI: 10.1016/j.micres.2021.126842.
Batish DR, Lavanya K, Singh HP, Kohli RK. 2007. Phenolic allelochemicals released by Chenopodium murale affect the growth, nodulation and macromolecule content in chickpea and pea. Plant Growth Regul 51: 119-128. DOI: 10.1007/s10725-006-9153-z.
Bhat MA, Kumar V, Bhat MA, Wani IA, Dar FL, Farooq I, Bhatti F, Koser R, Rahman S, Jan AT. 2020. Mechanistic insights of the interaction of plant growth-promoting rhizobacteria (PGPR) with plant roots toward enhancing plant productivity by alleviating salinity stress. Front Microbiol 11: 1952. DOI: 10.3389/fmicb.2020.01952.
Chouyia FE, Ventorino V, Pepe O. 2022. Diversity, mechanisms and beneficial features of Streptomyces in sustainable agriculture: A review. Front Plant Sci 13: 1035358. DOI: 10.3389/fpls.2022.1035358.
Dahal B, NandaKafle G, Perkins L, Brözel VS. 2017. Diversity of free-living nitrogen fixing Streptomyces in soils of the badlands of South Dakota. Microbiol Res 195: 31-39. DOI: 10.1016/j.micres.2016.11.004.
Dlamini SP, Akanmu AO, Babalola OO. 2022. Rhizospheric microorganisms: The gateway to a sustainable plant health. Front Sustain Food Syst 6: 925802. DOI: 10.3389/fsufs.2022.925802.
Fatmawati U, Meryandini A, Asih Nawangsih A, Tri Wahyudi A. 2019. Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth. Biodiversitas 20 (10): 2970-2977. DOI: 10.13057/biodiv/d201027.
Heng JLS, Hamzah H, Nejis NA. 2024. Diversity of Actinomycetes isolated from peat soil of undistrubed forest and pineapple plantation in Sessang, Sarawak. J Trop Biodivers Biotechnol 9 (2): 85390. DOI: 10.22146/jtbb.85390.
ISTA. 2018. International rules for seed testing, International Rules for Seed Testing. Zurich, Switzerland. DOI: 10.15258/istarules.2018.F.
Kaur S, Kalia A, Sharma S. 2024. Bioformulation of Azotobacter and Streptomyces for improved growth and yield of wheat (Triticum aestivum L.): A field study. J Plant Growth Regul 43: 2555-2571. DOI: 10.1007/S00344-024-11282-2/METRICS.
Khan S, Nadir S, Iqbal S, Xu J, Gui H, Khan A, Ye L. 2021. Towards a comprehensive understanding of free-living nitrogen fixation. Circ Agric Syst 1 (1): 1-11. DOI: 10.48130/cas-2021-0013.
Labeda DP, Goodfellow M, Brown R, Ward AC, Lanoot B, Vanncanneyt M, Swings J, Kim SB, Liu Z, Chun J, Tamura T, Oguchi A, Kikuchi T, Kikuchi H, Nishii T, Tsuji K, Yamaguchi Y, Tase A, Takahashi M, Sakane T, Suzuki KI, Hatano K. 2012. Phylogenetic study of the species within the family Streptomycetaceae. Antonie van Leeuwenhoek, Intl J Gen Mol Microbiol 101: 73-104. DOI: 10.1007/s10482-011-9656-0.
Li C, Chen X, Jia Z, Zhai L, Zhang B, Grüters U, Ma S, Qian J, Liu X, Zhang J, Müller C. 2024. Meta-analysis reveals the effects of microbial inoculants on the biomass and diversity of soil microbial communities. Nat Ecol Evol 8: 1270-1284. DOI: 10.1038/s41559-024-02437-1.
Majer HM, Ehrlich RL, Ahmed A, Earl JP, Ehrlich GD, Beld J. 2021. Whole genome sequencing of Streptomyces actuosus ISP-5337, Streptomyces sioyaensis B-5408, and Actinospica acidiphila B-2296 reveals secondary metabolomes with antibiotic potential. Biotechnol Rep 29: e00596. DOI: 10.1016/j.btre.2021.e00596.
Masganti M, Anwar K, Susanti MA. 2020. Potensi dan pemanfaatan lahan gambut dangkal untuk pertanian. Jurnal Sumberdaya Lahan 11 (1): 43-52. DOI: 10.21082/jsdl.v11n1.2017.43-52. [Indonesian]
Nair RM, Boddepalli VN, Yan MR, Kumar V, Gill B, Pan RS, Wang C, Hartman GL, Silva e Souza R, Somta P. 2023. Global status of vegetable soybean. Plants 12 (3): 609. DOI: 10.3390/plants12030609.
Pantoja-Guerra M, Valero-Valero N, Ramírez CA. 2023. Total auxin level in the soil-plant system as a modulating factor for the effectiveness of PGPR inocula: A review. Chem Biol Technol Agric 10 (1): 6. DOI: 10.1186/s40538-022-00370-8.
Sari WP, Adriani DE, Nisa C. 2021. Growth response of edamame soybean (Glycine max (L.) Merr.) with application of urea and Rhizobium biofertilizer on peat soil media. Trop Wetl J 7 (1): 17-24. DOI: 10.20527/twj.v7i1.100.
Steinauer K, Thakur MP, Emilia Hannula S, Weinhold A, Uthe H, van Dam NM, Bezemer TM. 2023. Root exudates and rhizosphere microbiomes jointly determine temporal shifts in plant-soil feedbacks. Plant Cell Environ 46 (6): 1885-1899. DOI: 10.1111/pce.14570.
Suárez-Moreno ZR, Vinchira-Villarraga DM, Vergara-Morales DI, Castellanos L, Ramos FA, Guarnaccia C, Degrassi G, Venturi V, Moreno-Sarmiento N. 2019. Plant-growth promotion and biocontrol properties of three Streptomyces spp. isolates to control bacterial rice pathogens. Front Microbiol 10: 209. DOI: 10.3389/fmicb.2019.00290.
Sumbul A, Ansari RA, Rizvi R, Mahmood I. 2020. Azotobacter: A potential bio-fertilizer for soil and plant health management. Saud J Biol Sci 27 (12): 3634-3640. DOI: 10.1016/j.sjbs.2020.08.004.
Tanasupawat S, Phongsopitanun W, Suwanborirux K, Ohkuma M, Kudo T. 2016. Streptomyces actinomycinicus sp. nov., isolated from soil of a peat swamp forest. Intl J Syst Evol Microbiol 66 (1): 290-295. DOI: 10.1099/ijsem.0.000716.
Upadhyay SK, Srivastava AK, Rajput VD, Chauhan PK, Bhojiya AA, Jain D, Chaubey G, Dwivedi P, Sharma B, Minkina T. 2022. Root exudates: Mechanistic insight of plant growth promoting rhizobacteria for sustainable crop production. Front Microbiol 13: 916488. DOI: 10.3389/fmicb.2022.916488.
Wagner MR, Roberts JH, Balint-Kurti P, Holland JB. 2020. Heterosis of leaf and rhizosphere microbiomes in field-grown maize. New Phytol 228 (3): 1055-1069. DOI: 10.1111/nph.16730.
Wahyudi AT, Priyanto JA, Fijrina HN, Mariastuti HD, Nawangsih AA. 2019. Streptomyces spp. from rhizosphere soil of maize with potential as plant growth promoter. Biodiversitas 20 (9): 2547-2553. DOI: 10.13057/biodiv/d200916.
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