Diversity of phyllospheric Actinomycetes in Liliaceae plants and their potential as growth inhibitors of Alternaria porri
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Wati C, Nawangsih AA, Wahyudi AT, Wiyono S, Munif A. 2023. Diversity of phyllospheric Actinomycetes in Liliaceae plants and their potential as growth inhibitors of Alternaria porri. Biodiversitas 24: 5234-5242. Actinomycetes are microorganisms belonging to a group of Gram-positive bacteria, that have the potential to act as biological control agents against various plant pathogens. Phyllospheric Actinomycetes particularly, can inhibit growth of various microorganisms, such as the fungus Alternaria porri Ell. Cif., which causes purple blotch disease in the shallot plants. These microbes thrive on the surface of leaves, particularly in plants belonging to Liliaceae family. Environmental conditions have been proven to greatly affect their diversity. Present investigation aims to determine diversity of phyllospheric Actinomycetes from Liliaceae plants and their potential to inhibit growth of A. porri. The methods used were to investigate, characterize, and quantify isolates from Liliaceae plants. An antagonistic test was conducted on the isolates to examine the proportion of A. porri fungus growth inhibition. The results showed that plants species Allium fistulosum and A. tuberosum were the most common hosts for phyllospheric actinomycetes. Morphological differences were found in shape, color, elevation, edges, surface, and hyphae size, as well as the type of spore violation. The surface texture of colonies was smooth, rough, powdery, and opaque, usually appearing after 7-12 days of isolation. The isolates also inhibited growth of A. porri, causing the mycelium thins, and a clear zone of inhibition between pathogens and Actinomycetes forms. Isolates BCW9 caused the highest suppression of fungus growth 57.78%. This study is the first experiment to evaluate the diversity of phyllosphere Actinomycetes from Liliaceae plants, which are able to control purple blotch disease caused by A. porri in Indonesia. The phyllospheric Actinomycetes were isolated from the same habitat of A. porri. These investigation show the prospect of phyllospheric Actinomycetes as biocontrol agents against another fungal diseases on leaves of another plants.
##plugins.themes.bootstrap3.article.details##
References
Anandan R, Dharumadurai D, Manogaran GP. 2016. An introduction to actinobacteria. In actinobacteria-basics and biotechnological applications; Dhanasekaran D, Jiang Y, Eds.; Intech Open Limited: London, UK. DOI:10.5772/62329.
Astuty E. 2017. Isolasi dan karakterisasi morfologi aktinomiset indigenus asal tanah gambut. Jurnal Ilmu Alam dan Lingkungan. 8 (16):7-15.
Balint P, Kurti. 2019. The plant hypersensitive response: concepts, control and consequences. Molecular Plant Pathology. 20(8): 1163–1178. DOI: 10.1111/mpp.12821.
Barka EA. Vatsa P, Sanchez L, Gaveau VN, Jacquard C, Klenk HP, Clément C, Ouhdouch Y, Van WGP. 2016. Taxonomy, physiology, and natural products of actinobacteria. Microbiol. 80:1–43.
Bernal MG, Córdova AIC, Saucedo PE, González MC, Marrero RM, Suástegui JMM. 2015. Isolation and in vitro selection of actinomycetes strains as potential probiotics for aquaculture. Veterinary World. 8(2):170-176. DOI: 10.14202/vetworld.2015.170-176.
Bonaldi M, Kunova A, Saracchi M, Sardi P, Cortesi P. 2014. Streptomycetes as biological control agents against basal drop. Acta Hort. 1044: 313-318.
Dikin A, Sijam K, Kadir J, Seman IA. 2006. Antagonistic bacteria against Schizophyllum Commune FR. in Peninsular Malaysia. Biotropia. 13(2): 111 – 121.
Djebaili R, Pellegrini M, Bernardi M, Smati M, Kitouni M, Gallo MD. 2021. Biocontrol activity of actinomycetes strains against fungal and bacterial pathogens of Solanum lycopersicum L. and Daucus carota L.: in vitro and in planta antagonistic activity. Biol. Life Sci. Forum 2021. 4(27):1-6. DOI: 10.3390/IECPS2020-08863.
Fadhilah QG, Santoso I, Yasman. 2021. The antagonistic activity of marine actinomycetes from mangrove ecosystem against phytopathogenic fungi Colletotrichum sp. KA. Biodiversitas. 22(2): 640-647. DOI: 10.13057/biodiv/d220216.
Fatimah, Suroiyah F, Solikha N, Rahayuningtyas ND, Surtiningsih T, Nurhariyati T, Ni’matuzahroh, Affandi M, Geraldi A, Thontowi A. 2022. Antimicrobial activity of actinomycetes isolated from mangrove soil in Tuban, Indonesia. Biodiversitas 23: 2957- 2965. DOI: 10.13057/biodiv/d230622.
Fatmawati U, Meryandini A, Nawangsih AA, Wahyudi AT. 2019. Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth. Biodiversitas 20: 2970-2977. DOI: 10.13057/biodiv/d201027.
Gopalakrishnan S, Srinivas V, Vidya MS, Rathore A. 2013. Plant growth-promoting activities of Streptomyces spp. in sorghum and rice. SpringerPlus. 574:1-8. DOI: 10.1186/2193-1801-2-574.
Helal IM. 2017. Control of damping-off disease in some plants using environmentally safe biocide. Pak. J. Bot., 49(1): 361-370.
Hersanti, Sudarjat, Damayanti A. 2019. Kemampuan Bacillus subtilis dan Lysinibacillus sp. dalam silika nano dan serat karbon untuk menginduksi ketahanan bawang merah terhadap penyakit bercak ungu (Alternaria porri (Ell.) Cif)). Jurnal Agrikultura. 30 (1): 8-16.
Kunova A, Bonaldi M, Saracchi M, Pizzatti C, Chen X, Cortesi P. 2016. Selection of Streptomyces against soil borne fungal pathogens by a standardized dual culture assay and evaluation of their effects on seed germination and plant growth. Microbiology. 16(272): 2-11. DOI 10.1186/s12866-016-0886-1.
Li Q, Chen X, Jiang Y, Jiang C. 2016. Morphological identification of actinobacteria. in actinobacteria-basics and biotechnological applications; Dhanasekaran D, Jiang Y, Eds.; IntechOpen Limited: London, UK. DOI: 10.5772/61461.
Nataliya V, Balashova, Juan A, Crosby, Lourdes Al Ghofaily, Scott C. 2006. Leukotoxin confers beta-hemolytic activity to Actinobacillus actinomycetemcomitans. Infect Immun. 74(4):2015–2021. DOI: 10.1128/IAI.74.4.2015- 2021.2006.
Ohike T, Maeda M, Matsukawa T, Okanami M, Kajiyama S, Ano T. 2018. In vitro and in vivo assay for assessment of the biological control potential of Streptomyces sp. KT. Journal of Plant Studies. 7(1):10-18. DOI:10.5539/jps.v7n1p10.
Olanrewaju OS, Babalola OO. 2018. Streptomyces: implications and interactions in plant growth promotion. Applied Microbiology and Biotechnology. DOI:10.1007/s00253-018- 09577-y.
Rante H, Alam G, Pakki E, Usmar U, Ali A. 2020. Identification and antibacterial activity of actinomycetes isolated from medicinal plant Andrographis Paniculata rhizosphere soil. Journal of Medical and Biological Sciences. 7(4): 1-7.
Ratnakomala S, Lisdiyanti P, Prayitno NR, Lestari ETY, Hastuti RD, Otoguro M, Widyastuti Y, Ando K, Sukara E. 2016. Diversity of actinomycetes from eka karya botanical garden, Bali. Biotropia. 23(1): 42 – 51. DOI: 10.11598/btb.2016.23.1.504.
Shannon CE, Weaver W. 1949. The mathematical theory of communication. Illinois (USA): University of Illinois Press.
Sreevidyaa M. Gopalakrishnan, Kudapab H, Varshney RK. 2016. Exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in chickpea. Journal of microbiology. 47(1): 85–95. DOI: 10.1016/j.bjm.2015.11.030.
Sukmawaty E, Sari SR, Masri M. 2020. Characterization of soil actinomycetes from malino pine forest rhizosphere of South Sulawesi. Elkawnie. 6(2): 315- 328. DOI:10.22373/ekw.v6i2.5383.
Sulistyani N, Akbar AN. 2014. Aktivitas isolat actinomycetes dari rumput laut (Eucheuma cottonii) sebagai penghasil antibiotik terhadap Staphylococcus aureus dan Escherichia coli. Jurnal Ilmu Kefarmasian Indonesia. 12(1): 1-9.
Utarti E, Suwanto A, Maggy T. Suhartono, Meryandini A. 2020. Identifikasi aktinomiset selulolitik dan xilanolitik indigenous. Berkala Sainstek. 7(1): 1-5.
Wahyudi AT, Priyanto JA, Fijrina HN, Mariastuti HD, Nawangsih AA. 2019. Streptomyces spp. from rhizosphere soil of maize with potential as plant growth promoter. Biodiversitas. 20: 2547-2553. DOI: 10.13057/biodiv/d200916.
Wijayanti E, Nawangsih AA, Tondok ET. 2021. Penapisan Aktinomiset Rizosfer Tanaman Liliaceae sebagai Agens Pengendali Hayati Fusarium oxysporum f. sp. Cepae. Jurnal Fitopatologi Indonesia. 17(6):225-232. DOI: 10.14692/jfi.17.6.225–232.
Wiraswati SM, Rusmana I, Nawangsih AA, Wahyudi AT. 2019. Antifungal activities of bacteria producing bioactive compounds isolated from rice phyllosphere against Pyricularia oryzae. Journal of Plant Protection Research. 59(1): 86–94. DOI: 10.24425/jppr.2019.126047.
Vorholt JA. 2012. Microbial life in the phyllosphere. Nature. 10:828-840. DOI: 10.1038/nrmicro2910.
Zhu W, MaGbanua MM, White FF. 2000. Identification of two novel hrp-associated genes in the hrp gene cluster of Xanthomonas oryzae pv. oryzae. J Bacteriol. 182 (7): 1844– 1853. DOI: 10.1128/jb.182.7.1844-1853.2000.
Most read articles by the same author(s)
- DOVA KELVIN MESRIAN, WIDYA ESTI PURWANINGTYAS, RIKA INDRI ASTUTI, AKHMAD ENDANG ZAINAL HASAN, ARIS TRI WAHYUDI, Methanol pigment extracts derived from two marine actinomycetes exhibit antibacterial and antioxidant activities , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 10 (2021)
- UMI FATMAWATI, ANJA MERYANDINI, ABDJAD ASIH NAWANGSIH, ARIS TRI WAHYUDI, Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 10 (2019)
- HENY HERNAWATI, SURYO WIYONO, SUGENG SANTOSO, Leaf endophytic fungi of chili (Capsicum annuum) and their role in the protection against Aphis gossypii (Homoptera: Aphididae) , Biodiversitas Journal of Biological Diversity: Vol. 12 No. 4 (2011)
- RAHMAD LINGGA, SRI BUDIARTI, IMAN RUSMANA, ARIS TRI WAHYUDI, Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 7 (2020)
- MAYA SARI, ABDJAD ASIH NAWANGSIH, ARIS TRI WAHYUDI, Rhizosphere Streptomyces formulas as the biological control agent of phytopathogenic fungi Fusarium oxysporum and plant growth promoter of soybean , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 6 (2021)
- PRAMESITA PRAWADIKA APSARI, SRI BUDIARTI, ARIS TRI WAHYUDI, Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 5 (2019)
- RURY ERYNA PUTRI, NISA RACHMANIA MUBARIK, LAKSMI AMBARSARI, ARIS TRI WAHYUDI, Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 9 (2021)
- NEPTU ISLAMY RAHARJA, WIDANARNI, ARIS TRI WAHYUDI, Marine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by Vibrio parahaemolyticus , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 11 (2019)
- ARIS TRI WAHYUDI, JEPRI AGUNG PRIYANTO, HANIFA NUR FIJRINA, HIMA DEWI MARIASTUTI, ABDJAD ASIH NAWANGSIH, Streptomyces spp. from rhizosphere soil of maize with potential as plant growth promoter , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 9 (2019)