Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth
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Abstract. Fatmawati U, Meryandini A, Nawangsih AA, Wahyudi AT. 2019. Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth. Biodiversitas 20: 2970-2977. Actinomycetes which colonized plant rhizosphere has a vital role in improving plant growth by producing Indole-3-Acetic Acid (IAA). The aim of this study was to evaluate the potency of actinomycetes isolated from soybean rhizosphere as promoter agents for plant growth in vitro. Fifty actinomycetes isolates were successfully isolated from soybean rhizosphere. Based on the colorimetric methods, 35 isolates can produce IAA in various concentration, in the range of 0.46-30.6 mg/L. Seed germination assay using Ragdoll methods revealed that 26 isolates significantly promoted germination parameters, including the hypocotyl and the radicular length, the number of the lateral roots, and dry weight of the plant. Also, 14 from 26 isolates showed phosphate solubilizing activity in different phosphate-solubilizing index ranging from 1.25-2.62. Eight isolates were able to grow in N-free medium, indicating that these isolates have the ability in fixing nitrogen. Out of 23 from 26 isolates were detected to produce siderophore. All the tested isolates show chitinase production except ASR 55. Based on the observed parameters, it showed that there are four potential isolates (ASR 46, ASR 58, ASR 75 and ASR 76) as promising plant-growth promoters, phosphate solubilizer, nitrogen fixer, siderophore and chitinase producer. Based on the result of 16S rRNA sequence analysis, four potential isolates were identified as Streptomyces spp. in different taxa of strains and species.
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References
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Sadeghi A, Karimi E, Dahazi PA, Javid MG, Dalvand Y, Askari H. 2012. Plant growth promoting activity of an auxin and siderophore producing isolate of Streptomyces under saline soil condition. World Journal of Microbiology Biotechnology 28:1503–9.
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Sari WE, Solihin DD, Lestari Y. 2014. Identification of endophytic actinomycetes from Indonesian rice plant based on 16S rRNA and nifH genes analyses. Advances in Environmental Biology 8(7) : 2357-2365
Shimizu M. 2011. Endophytic aktinomisetes: biocontrol agents and growth promoters. Maheshwari DK [editorial]. Bacteria in Agrobiology: Plant Growth Responses. Springer Berlin Heidelberg. 201-220.
Shreevidya M, Gopalakrishnan S, Kudapa H, VASRhney NK. 2016. Exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in chickpea. Brazilian Journal of Microbiology 47: 85-95
Wahyudi AT, Priyanto JA, Fijrina HN, Mariastuti HD, Nawangsih AA. 2019. Streptomyces spp. from rhizosphere soil of maize with potential as plant growth promoter. Biodiversitas 20 (9): 2547-2553
Wiraswati SM, Rusmana I, Nawangsih AA, Wahyudi AT. 2019. Antifungal activity of bacteria producing bioactive compound isoated from rice phyllosphere against Pyricularia oryzae. Journal of Plant Protection Research 59 (1): 86-94.
Yandegeri MS, Malviya N, Solanki MK, Shrivastava P, Sivakumar N. 2015. Chitinolytic Streptomyces vinaceusdrappus S5MW2 isolated from Chilika lake, India enhances plant growth and biocontrol efficacy through chitin supplementation against Rhizoctonia solani. World Journal of Microbiology and Biotechnology 31 (8) : 1217-1225
Aldillah R. 2015. Soybean Production and Consumption Prediction in Indonesia. Jurnal Ekonomi Kuantitatif Terapan 8(1): 9-23
Amini J, Agapoor Z, Ashengroph M. 2016. Evaluation of Streptomyces spp. against Fusarium oxysporum f. sp. Ciceris for the management of chickpea wilt. Journal of Plant Protection Research 56 (3): 257-264.
Anwar S, Ali B, Sajid I. 2016. Screening of rhizospheric actinomycetes for various in-vitro and in-vivo plant growth promoting (PGP) traits and for agroactive compound. Fontiers in Microbiology 7: 1334
Balitkabi.2016. Balitbangtan gelar teknologi budidaya kedelai Grobogan 3 ton per hektar [internet]. Malang (ID): Balitkabi [downloaded at 2016 Sept 27]. Available at: http://balitkabi.litbang.pertanian.go.id/kilas-litbang/2166-balitbangtan-gelar-teknologi-budidaya-kedelai-grobogan-3-ton-per-hektar.html
Chen YP, Rekha PD, Arun AB, Shen FT, Lai WA, Young CC. 2006. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Applied Soil Ecology 34: 33-41
Barucha UD, Patel KC, Trivedi UB. 2013. Antifungal activity of chatecholate type siderophore produce by Bacillus sp. International Journal Research of Pharmaceutical Science 4 (4): 528
Franco-Correa MF, Quintana A, Duque C, Suarez C, Rodríguez M X, Barea JM. 2010. Evaluation of actinomycete strains for key traits related with plant growth promotion and mycorrhiza helping activities. Applied Soil Ecology 45:209-217.
De Fretes CE, Sembiring L, Purwestri YA. 2013. Characterization of Streptomyces spp. producing indole-3-acetic acid as biostimulant agents. Indonesian Journal of Biotechnology 18 (2): 83-91
El-Tarabily KA. 2008. Promotion of tomato (Lycopersicon esculentum Mill.) plant growth by rhizosphere competent 1-amino cyclo propane-1-carboxylic acid deaminase– producing Streptomycete aktinomisetes. Plant Soil 308: 161–74
Gopalakrishnan S, Vadlamudi S, Vidya MS, Rathore A. 2013. Plant growth-promoting activities of Streptomyces spp. in sorghum and rice. SpringerPlus. 2(574): 1-8.
Gopalakrishnan S, Pande S, Sharma M, Humayun P, Kiran BK, Sandeep D. 2011. Evaluation of aktinomisete isolates obtained from herbal vermicompost for biologicalcontrol of Fusarium wilt of chickpea. Crop Protection 30:1070–8.
Gtari M, Gtari FG, Nouioui I, Beauchemin N, Tisa LS. 2012. Phylogenetic perspectives of nitrogen-fixing actinobacteria. Archieve Microbiology 194 (1): 3-11
Hamdali H, Bouizgarne B, Hafidi M, Lebrihi A, Virolle MJ, Ouhdouch Y. 2008. Screening for rock phosphate solubilizing Actinomycetes from Moroccan phosphate mines. Applied Soil Ecology 38:12–19
Hayakawa M, Nonomura H. 1987. Humic Acid-Vitamin Agar, a new medium for the selective isolation of soil actinomycet. Journal of Fermentation Technology 65 (5): 501-509
Khamna S, Yokota A, Peberdy JF, Lumyong S. 2010. Indole-3-acetic acid production by Streptomyces sp. isolated from some Thai medicinal plant rhizosphere soils. EurAsian Journal of Biology Science 4: 23-32
Louden BC, Haarmann D, Lynne AM. 2011. Use of Blue Agar CAS Assay for Siderophore Detection. Journal of Microbiology and Biology Education 12 (1): 51-53.
Martina K, Jan K, Tamas F, Ladislav C, Marek O, Genevieve LG, Yvan ML, MASReta SM. 2008. Development of a 16S rRNA gene-based prototype microarray for the detection of selected actinomycetes genus. Antonio Van Leeuwenhoek 94:439-453.
Mohite B. 2013. Isolation and Characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth. Journal of Soil Science and Plant Nutrition 13(3): 638-649
Palaniyandi SA, Yang SH, Zhang L, Suh JW. 2016. Effects of actinobacteria on disease suppression and growth promotion. Applied Microbiology and Biotechnology 97 (22) : 9621-36.
Patten CL, Glick BR. 2002. Role of Pseudomonas putida indole acetic acid in development of the host plant root system. Applied and Environmental Microbiology 68 (8): 3795-3801
Poovarasan S, Mohandas S, Paneerselvam P, Sharita B, Ajay KM. 2013. Mycorrhizae colonizing aktinomisetes promote plant growth and control bacterial blight disease of pomegranate (Punica granatum L.cv Bhagwa). Crop Protection 53: 175-181
Rahman A, Sitepu IR, Tang SY, Hashidoko Y. 2010. Salkowski’s reagent test as a primary screening index for functionalities of rhizobacteria isolates from wild dipterocarp saplings growing naturally on medium-strongly acidic tropical peat soil. Bioscience, Biotechnology, Biochemistry 74 (11) : 2202-2208
Sàez-Plaza P, Navaz JM, Wybraniec S, Michalowski T, Asuero AG. 2013. An overview of the Kjeldahl method of nitrogen determination. Part II. Sample Preparation, Working Scale, Instrumental Finish, and Quality Control. Critical Reviews in Analytical Chemistry.43: 224–272
Sadeghi A, Karimi E, Dahazi PA, Javid MG, Dalvand Y, Askari H. 2012. Plant growth promoting activity of an auxin and siderophore producing isolate of Streptomyces under saline soil condition. World Journal of Microbiology Biotechnology 28:1503–9.
Saif S, Khan MS, Zaidi A, Ahmad E. 2014. Role of phosphate-solubilizing actinomycetes in plant growth promotion: current perspective. In: Khan MS, Zaidi A, Musarrat J (eds) Phosphate Solubilizing Microorganisms Principle and Application of Microphos Technology. Springer International Publishing. Switzerland
Sari WE, Solihin DD, Lestari Y. 2014. Identification of endophytic actinomycetes from Indonesian rice plant based on 16S rRNA and nifH genes analyses. Advances in Environmental Biology 8(7) : 2357-2365
Shimizu M. 2011. Endophytic aktinomisetes: biocontrol agents and growth promoters. Maheshwari DK [editorial]. Bacteria in Agrobiology: Plant Growth Responses. Springer Berlin Heidelberg. 201-220.
Shreevidya M, Gopalakrishnan S, Kudapa H, VASRhney NK. 2016. Exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in chickpea. Brazilian Journal of Microbiology 47: 85-95
Wahyudi AT, Priyanto JA, Fijrina HN, Mariastuti HD, Nawangsih AA. 2019. Streptomyces spp. from rhizosphere soil of maize with potential as plant growth promoter. Biodiversitas 20 (9): 2547-2553
Wiraswati SM, Rusmana I, Nawangsih AA, Wahyudi AT. 2019. Antifungal activity of bacteria producing bioactive compound isoated from rice phyllosphere against Pyricularia oryzae. Journal of Plant Protection Research 59 (1): 86-94.
Yandegeri MS, Malviya N, Solanki MK, Shrivastava P, Sivakumar N. 2015. Chitinolytic Streptomyces vinaceusdrappus S5MW2 isolated from Chilika lake, India enhances plant growth and biocontrol efficacy through chitin supplementation against Rhizoctonia solani. World Journal of Microbiology and Biotechnology 31 (8) : 1217-1225
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