Marine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by Vibrio parahaemolyticus
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Abstract. Raharja NI, Widanarni, Wahyudi AT. 2019. Marine sponge-associated bacteria as biocontrol agents of vibriosis on whiteleg shrimp caused by Vibrio parahaemolyticus. Biodiversitas 20: 3164-3169. Sponge-associated bacteria are commonly known as an excellent source of microbial's bioactive compounds attributed to biological properties, including antibacterial activity. In our previous study, 12 isolates from sponge Aaptos sp. and Hyrtios have been screened to have anti-Vibrio spp. activities in vitro. The objective of this study was to evaluate these bacterial isolates for controlling Vibrio parahaemolyticus infection in vivo, identify the most potential isolate, and analyze chemical composition of bacterial crude extract. As tested by pathogenicity assay on post-larvae of whiteleg shrimp stadia PL 10, all 12 isolates were not pathogenic bacteria as indicated by the survival rate of shrimps ranging from 80 ± 8.1 - 93 ± 4.7%. Conversely, more than 50% died after inoculated with pathogenic V. parahaemolyticus. Interestingly, all isolates could increase the survival rate of shrimps infected with V. parahaemolyticus (105 cells/mL) reach of 80 ± 0.8 - 91 ± 0.4%, nearly 40% higher than the positive control, as evaluated by challenge test. The best anti-Vibrio activity showed by D2.Z13 isolate which could improve the survival rate up to 91.11 ± 0.4%. 16S-rRNA-based identification showed that this isolate was closely related to Pseudomonas aeruginosa 1-4-b-9 strain. The extract derived from this isolate contained some major compounds such as 4,8-dihydroxy-2-2 (1’-hydroxyheptyl) and 1,2,4-oxadiazol which have been reported as antibacterial compounds. Our study indicates that these bacterial isolates could be developed as biocontrol agents of vibriosis in whiteleg shrimp.
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References
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Wahyudi AT, Priyanto JA, Maharsiwi W, Astuti RI. 2018. Screening and characterization of sponge-associated bacteria producing bioactive compounds anti-Vibrio sp. American J of Biochemistry and Biotechnology 14(3): 221-229.
Wang L, Chen Y, Huang H, Huang Z, Chen H, Shao Z. 2015. Isolation and identi?cation of Vibrio campbellii as a bacterial pathogen for luminous vibriosis of Litopenaeus vannamei. Aquaculture Research 46: 395–404.
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Aguirre-Guzmán G, López-Acevedo EA, Vázquez-Sauceda MDL. 2013. Vibrio harveyi effect under survival of Litopenaeus vannamei larvae. Scientia Agropecuaria 4: 121-127.
Azman A, Othman L, Fang C, Chan K, Goh B, Lee. 2017. Antibacterial, anticancer and neuroprotective activities of rare actinobacteria from mangrove forest soils. Indian J Microbiol 57(2): 177–187.
Chatterjee S, Haldar S. 2012. Vibrio related diseases in aquaculture and development of rapid and accurate identification methods. J Marine Sci Res Dev 002: 1-7.
Cobo ML, Sonnenholzner S, Wille M, Sorgeloos P. 2012. Ammonia tolerance of Litopenaeus vannamei (Boone) larvae. Aquac Res 1–6.
Cunha FS. Nogueira JM, Aguiar AP. 2018. Synthesis and antibacterial evaluation of 3,5-Diaryl-1,2,4 oxadiazole Derivatives. J Braz Chem Soc 29(11): 2405-2416.
Drais AA, Ahmad A, Alwan MG, Sahrani FK. 2018. Antimicrobial resistance and Plasmid profile of Vibrio alginolyticus isolated from Malaysian seawater. International J of ChemTech Res 11(10): 375-83.
Ghenem L, Elhadi N. 2018. Isolation, molecular characterization, and antibiotic resistance patterns of Vibrio parahaemolyticus isolated from coastal water in the Eastern Province of Saudi Arabia. J of Water and Health 16(1): 57-69.
Gode-Potraz C, McCarter LL. 2011.Qourum sensing and silencing in Vibrio parahaemolyticus. J of Bacteriol 193(16): 4224-4237.
Gohar YM, El-Naggar MMA, Barakat KM. 2010. Characterization of marine Burkholderia cepacia antibacterial agents. J Nat Prod 3: 86-94.
Ireri NF, Hamadi BI, Wanyoike, Kachiru R. 2015. Characterization, enzymatic activity, and secondary metabolites of fungal isolates from lake sonachi in Kenya. IOSR J of Pharm and Biological Sciences 10(2): 65-76.
Joshi J, Srisala J, Truong VH, Chen IT, Nuangsaeng B, Suthienkul O, Lo CF, Flegel TW, Sritunyalucksana K, Thitamadee S. 2014. Variation in Vibrio parahaemolyticus isolates from a single Thai shrimp farm experiencing an outbreak of acute hepatopancreatic necrosis disease (AHPND). Aquaculture 428-429: 297-302.
Le HP, Corteel M, Nguyen CT, Nauwynck H, Pensaert M, Alday-Sanz V, et al. 2009. Effect of dose and challenge routes of Vibrio spp. on co-infection with white spot syndrome virus in Penaeus vannamei. J Aquaculture 290(1-2):61–8.
Li H, Lee BC, Kim TS, Bae KS, Hong J, Choi SH, et al. 2008. Bioactive Cyclic Dipeptides from a Marine Sponge-Associated Bacterium, Psychrobacter sp. Biomolecules and Therapeutics 16: 356-363.
Li M, Li G, Zhang K. 2019. Non-volatile metabolites from Trichoderma spp. Metabolites 9(58): 1-24.
Managamuri U, Vijayalakshmi M, Ganduri VSRK, Rajulapati SB, Bonigala B, Kalyani BS, et al. 2017. Isolation, identification, optimization, and metabolite profiling of Streptomyces sparsus VSM-30. 3 Biotech 7(217): 1-19.
Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC, Hiom SJ, et al. 1998. Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 64: 795–799.
Nazim K, Sherwani SK, Khan MU, Kausar R, Rizvi G. 2014. Antibacterial activity of marine sponge collected from Sunhari beach. Fuuast J Biol 4(2): 233-236.
Prastya ME, Astuti RI, Batubara I, Wahyudi AT. Bacillus sp. 2019. SAB E-41-derived extract shows antiaging properties via ctt1-mediated oxidative stress toleranc respone in yeast Schizosaccharomyces pombe. Asian Pac J of Tropical Biomedicine 8(11): 533-539.
Prastya ME, Astuti RI, Batubara I, Wahyudi AT. 2019. Antioxidant, Antiglycation and in vivo Antiaging Effects of Metabolite Extracts from Marine Sponge-associated Bacteria. Indian J Pharm Sci 81(2):344-353.
Priyanto JA, Astuti RI, Nomura J, Wahyudi AT. 2017. Bioactive Compounds from Sponge Associated Bacteria: Anticancer Activity and NRPS-PKS Gene Expression in Different Carbon Sources. American J of Biochemistry and Biotechnology 13(4): 148-156.
Rini AF, Yuhana M, Wahyudi AT. 2017. Potency of sponge-associated bacteria producing bioactive compounds as biological control of vibriosis on shrimp. JAkuakultur Indonesia 16(1): 41–50.
Saha S, Thavasi R, Jayalakshmi S. 2008. Phenazine pigments from Pseudomonas aeruginosa and their application as antibacterial agent and food colourants. Res J Microbiol 3:122–128.
Santos OCS, Pontes PVML, Santos JFM, Muricy G, Giambiagi-deMarval M, Laport MS. 2010. Isolation, characterization and phylogeny of sponge associated bacteria with antimicrobial activities from Brazil. J Res Microbiol 161: 604–612.
Song S, Fu S, Sun X, Li P, Wu J, Dong T, et al. 2018. Identification of cyclic dipeptides from Escherichia coli as new antimicrobial agents against Ralstonia Solanacearum. Molecules 23(214): 1-14.
Soto-Rodriguez SA , Olvera RL, Gomez-Gil B, Betancourt-Lozano M, Morales-Covarrubias MS. 2015. Field and Experimental Evidence of Vibrio parahaemolyticus as the Causative Agent of Acute Hepatopancreatic Necrosis Disease of Cultured Shrimp (Litopenaeus vannamei) in Northwestern Mexico. Appl Environ Microbiol 81(5): 1689–1699.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: Molecular Evolutionary Genatics Analysis Version 6.0. Mol Biol Evol 30(12): 2725–2729.
Tangjitjaroenkun J. 2018. Evaluation of antioxidant, antibacterial, and gas chromatography-mass spectrometry analysis of ethyl acetate extract of Streptomyces omiyaensis SCH2. Asian J Pharm Clin Res 11(7): 271-276.
Thomas T, Moitinho-Silva L, Lurgi M, Björk JR, Easson C, Astudillo-García C, et al. 2016. Diversity, structure and convergent evolution of the global sponge microbiome. Nat Commun 7:11870.
Vidal MAV, Pessôa MN, Santos FL, Mendes P, Mendes ES. 2018. Probiotic potential of Bacillus cereus against vibrio spp. in post-larvae shrimps. Mossoró 31(2): 495 –503.
Wahyudi AT, Priyanto JA, Maharsiwi W, Astuti RI. 2018. Screening and characterization of sponge-associated bacteria producing bioactive compounds anti-Vibrio sp. American J of Biochemistry and Biotechnology 14(3): 221-229.
Wang L, Chen Y, Huang H, Huang Z, Chen H, Shao Z. 2015. Isolation and identi?cation of Vibrio campbellii as a bacterial pathogen for luminous vibriosis of Litopenaeus vannamei. Aquaculture Research 46: 395–404.
Zheng Y, Yu M, Liu J, Qiao Y, Wang L, Li Z, Zhang X ,Yu M. 2017. Bacterial community associated with healthy and diseased pacific white shrimp (Litopenaeus vannamei) larvae and rearing water across different growth stages. Frontiers in Microbiol 8: 1362.
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