Diversity of capsaicin content, quantitative, and yield components in chili (Capsicum annuum) genotypes and their F1 hybrid




Abstract. Sahid ZD, Syukur M, Maharijaya A. 2020. Genetic diversity of capsaicin content, quantitative, and yield component in chili (Capsicum annuum) and their F1 hybrid. Biodiversitas 21: 2251-2257. Chili (Capsicum annuum L.) is one of the horticultural plants that have many benefits. The benefit of chili was determined by pungency level of its fruit. Pungency level of the chili is due to the capsaicin content in fruit. Information about the genetic diversity of capsaicin is still rarely available. The aims of this study were to obtain diversity information on quantitative, yield component, and capsaicin content, and to analyze the correlation among chili genotypes based on their morphological characters. This study used Randomized Complete Block Design with three replications. The genetic material used in this study consisted of 21 genotypes consisting of 6 genotypes of chili elders and 15 hybrid F1 genotypes resulting from their crossing. Six genotypes of the chili parents are C5, F6074, F9160291, Yuni, Bara, and Giant. 15 hybrid F1 genotypes used in this study are C5 x Bara, C5 x F6074, C5 x Yuni, C5 x Giant, C5 x F9160291, Bara x F6074, Bara x Yuni, Bara x Giant, Bara x F9160291, F6074 x Yuni, F6074 x Giant, F6074 x F9160291, Yuni x Giant, Yuni x F9160291, and Giant x F9160291. The observation was made on the variables of quantitative, yield, and capsaicin components on chili. The results showed that the highest capsaicin content only was found in Bara x F9160291. The results of scatterplot analysis showed that the highest capsaicin and yield component was found in BaraxF6074 and C5 x Yuni genotype. The results of cluster analysis showed that chili was clustered into three color groups. The character of capsaicin content is negatively correlated and very different from fruit weight, fruit diameter, fruit length, thick fruit flesh, total amount of fruit per plant, and fruit weight per plant.


[IPGRI] International Plant Genetic Resources Institute. 1995. Descriptors for Capsicum (Capsicum spp.). Italia. 51p.
Claver AG, Ramiro GO, Ana AF, Maria SAA. 2007. Inheritance of capsaicin and dihydrocapsaicin, determined by HPLC-ESI/MS, in an intraspesific cross of Capsicum annuum L. J Agric Food Chem 55: 6951 – 6957.
Guzman I, Bosland PW, O’Connell MA. 2011. Heat, color, and flavor compounds in Capsicum fruit. In: Gang, D.R. (Ed.), The Biological Activity of Phytochemicals, Recent Advances in Phytochemistry 41. Springer: 109 – 126.
Istiqlal MRA, Syukur M, Wahyu Y. 2018. Inheritance and combining ability studies for yield and yield attributing traits of crossing big and curly fruit lines in chilli (Capsicum annuum L.). IOP Conf. Series: Earth and Environmental Science 196. doi :10.1088/1755-1315/196/1/012012.
Kumar BK, Munshi AD, Joshi S, Kaur C. 2003. Correlation and path coefficient analysis for yield and biochemical characters in chilli (Capsicum annuum L.). Capsicum and Eggplant newsletter: 12-23.
Lee J, Park SJ, Hong SC, Han JH, Choi D, Yoon JB, Havey M. 2016. QTL mapping for capsaicin and dihydrocapsaicin content in a population of Capsicum annuum ‘NB1’ 9 Capsicum chinense ‘Bhut Jolokia’. Plant Breed. 135: 376–383.
Lingga L. 2012. Health secret of pepper. Jakarta (ID): Elex Media Komputindo.
Orobiyi A, Dansi A, Assogba P, Loko LY, Dansi M, Vodouhe R, Akouegninou A, Sanni A. 2013. Chilli (Capsicum annuum L.) in southern Benin: production constraints, varietal diversity, preference criteria and participatory evaluation. Int Res J Agric Soi Sci 3 (4): 107 - 120.
Othman ZA, Hadj Ahmed YB, Habila MA, Ghafar AA. 2011. Determination of capsaicin and dihydrocapsaicin in capsicum fruit samples using high performance liquid chromatography. Molecules 16: 8919 - 8929.
Ritonga AW, Chozin MA, Syukur M, Maharijaya A, Sobir. 2018. Short Communication: Genetic variability, heritability, correlation, and path analysis in tomato (Solanum lycopersicum) under shading condition. Biodiversitas 19 (4): 1527 - 1531.
Ritonga AW, Syukur M, Chozin MA, Maharijaya A, Sobir. 2019. Different of selection response, genetic advance, and number of transgressive segregants resulting of crossed between shade-loving tomato x shade-sensitive tomato. Comm Hort J. 3 (1): 32 - 38.
Saing B and Reni M. 2018. Pemanfaatan capsaicin dari cabai dalam menghadapi fluktuasi harga cabai di pasaran. J Abdimas UBJ: 40 - 46.
Stewart C Jr, Kang BC, Liu K, Mazourek M, Moore SL, Yoo EY, Kim BD. 2005. The Pun1 gene for pungency in pepper encodes a putative acyltransferase. Plant J. 42: 675 - 688.
Stewart C Jr, Mazourek M, Stellari GM, O’Connell M, Jahn M. 2007. Genetic control of pungency in C. chinense via the Pun1 locus. J Exp Bot 58: 979 – 991.
Sukrasmo, Kusmardiyani S, Tarini S, Sugiarso NC. 1997. Kandungan Kapsaisin dan dihidrokapsaisin pada berbagai buah Capsicum. Bandung (ID): FMIPA ITB. J MS (2): 28 - 34.
Syukur M, Sujiprihati S, Yunianti R, Kusumah DA. 2010. Yield evaluation of pepper hybrids and their adaption at four location in two years. J Agron Indonesia. 38 (1): 43 – 51.
Todd PH, Besinger MG, Biftu T. 1977. Determination of pungency due to Capsicum by gas-liquid chromatography. J Food Sci. 42: 660 – 665.
Yarnes SC, Ashrafi H, Reyes-Chin-Wo S, Hill TA, Stoffel KM, Van Deynze A. 2013. Identification of QTLs for capsaicinoids, fruit quality, and plant architecture-related traits in an interspecific Capsicum RIL population. Genome 56: 61–74.
Yora M, Syukur M, Sobir. 2018. Characterization of phytochemicals and yield components in various okra (Abelmoschus esculentus) genotypes. Biodiversitas 19 (6): 2323-2328.
Zewdie Y, Bosland PW. 2000. Capsaicinoid inheritance in an interspecific hybridization of Capsicum annuum x C. chinense. J Am Soc Hort Sci 125: 448 - 453.