Genetic diversity of eucalypts for germplasm conservation in Forest Area with the Special Purpose of Mount Bromo, Karanganyar, Indonesia




Abstract. Rahayu, Fatimah, Wiwoho J, Firdaus SU, Pujiyono, Marimin, Arianto DP, Pramono A. 2021. Genetic diversity of eucalypts for germplasm conservation in Forest Area with the Special Purpose of Mount Bromo, Karanganyar, Indonesia. Biodiversitas 22: 4223-4235. As a repository of a gene pool, eucalypts germplasm enriches biodiversity, maintains ecosystem sustainability, and aids in conservation. Therefore, this study aims to analyze the genetic diversity of eucalypts (Corymbia and Eucalyptus) for the development of germplasm conservation in Forest Area with the Special Purpose (KHDTK) Bromo Forest, Karanganyar, Indonesia. In this study, 14 simple sequence repeat (SSR) markers were used to assess the genetic diversity among 20 accessions (Corymbia and 5 Eucalyptus species) from Central and West Java. Subsequently, the genetic parameters were measured and a phylogenetic tree was constructed. The result showed that the SSR markers have high variability, although they belong to different genera. Furthermore, the genetic diversity showed 49 alleles with an average of 3 alleles per locus, while the polymorphism information content (PIC) values were 0.55. There were 4 SSR markers (EMBRA13, EMBRA8, EMCRC11, and EMBRA2) with high PIC value, while the gene diversity (He) of Corymbia and 5 Eucalyptus showed a low level of genetic diversity. The genetic relationship and population structure were divided into genera Corymbia and Eucalyptus. For further application, the eucalypt cultivated in the KHDTK Bromo Forest can contribute as a reference set and 14 SSR markers as a potential marker in combination with morphological characterization to generate a database for germplasm management and conservation.


Apriyanto D, Kusnandar. 2020. Kajian potensi dan strategi pengembangan wisata alam di kawasan hutan dengan tujuan khusus (KHDTK) Gunung Bromo. Jurnal Belantara. 3(1):80-88. DOI:
Brondani RPV, Brondani C, Tarchini R, Grattapaglia D. 1998. Development, characterization and mapping of microsatellite markers in Eucalyptus grandis and E. urophylla. Theoretical and Applied Genetics. 97: 816-827.
Burke MK, Long AD. 2012. Perspective: What paths do advantageous alleles take during short-term evolutionary change? Molecular Ecology. 21: 4913-4916.
Costa J, Vaillancourt RE, Dorothy AS, Rebecca CJ, Cristina M. 2017. Microsatellite analysis of population structure in Eucalyptus globulus. Genome 60: 770–777.
Earl DA, vonHoldt BM. 2012. STRUCTURE HARVESTER: A website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv Genet Resour 4:359–361.
Goodine T, Oelgemoller M. 2020. Corymbia citriodora: A Valuable Resource from Australian Flora for the Production of Fragrances, Repellents, and Bioactive Compounds.
Grattapaglia D, Mamani EMC, Silva-Junior OB, Faria DA. 2015. A novel genome-wide microsatellite resource for species of Eucalyptus with linkage-to-physical correspondence on the reference genome sequence. Molecular Ecology Resources. 15:437–448. doi: 10.1111/1755-0998.12317.

Most read articles by the same author(s)

1 2 > >>