Antimicrobial activity of actinomycetes isolated from mangrove soil in Tuban, East Java, Indonesia
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Fatimah, Suroiyah F, Solikha N, Rahayuningtyas ND, Surtiningsih T, Nurhariyati T, Ni’matuzahroh, Affandi M, Geraldi A, Thontowi A. 2022. Antimicrobial activity of actinomycetes isolated from mangrove soil in Tuban, Indonesia. Biodiversitas 23: 2957-2965. This study aims to isolate, identify, and investigate the antimicrobial activity of actinomycetes isolated from mangrove soil of the Mangrove Center Tuban in Jenu Village, Tuban District, Indonesia. Soil sampling was carried out with a purposive sampling technique. The dry-heating and pour plate methods were used to isolate actinomycetes. Four isolates (LIB.2; LIA.4; LIA.9; and LIA.10) were selected and tested for their antimicrobial activities against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Candida albicans ATCC 10231 using disc diffusion method. Colony morphology characterization and Gram staining were then conducted on the 4 isolates. The identification of LIB.2 was performed by biochemical assay and 16S rRNA gene sequence analysis. The results showed that crude antimicrobial extracts (CACs) of all isolates inhibited the growth of E. coli but not S. aureus and C. albicans. Isolate LIB.2 showed the highest inhibition zone with a diameter of 15,65 ± 2,33 mm, followed by LIA.9 (10,19 ± 2,78 mm), LIA.4 (9,80 ± 0,47), and LIA.10 (8,75 ± 0,42 mm). The most potential isolate was identified as a member of Streptomyces genera. Based on biochemical assay and 16S rRNA gene sequence analysis, LIB.2 was identified as Streptomyces vellosus strain LIB.2.
##plugins.themes.bootstrap3.article.details##
References
Bertrand JC, Caumette P, Lebaron P, Matheron R, Normand P, Ngando TS. 2015. Environmental Microbiology: Fundamentals and Applications Springer London 187. DOI: 10.1007/978-94-017-9118-2
Fatiqin A. 2011. Uji Potensi Actinomycetes Sebagai Penghasil Antibiotik yang Diisolasi dari Tanah Rhizosfer Mangrove Wonorejo Surabaya. [Tesis]. Universitas Airlangga, Surabaya. [Indonesia].
Hong K, Gao A, Xie Q, Gao H, Zhuang L, Lin H, Hai P Y, Jia L, Yao X, Goodfellow M, Ruan J. 2009. Actinomycetes for marine drug discovery isolated from mangrove soils and plantsin china. Marine Drugs. 7: 24-44. DOI: 10.3390/md7010024
Katili AS, Retnowati Y. 2017. Isolation of actinomycetes from mangrove ecosystem in torosiaje gorontalo Indonesia. Biodiversitas. 18(2): 826-833. DOI: https://doi.org/10.13057/biodiv/d180259
Khanna MR, Solanski, Lal R. 2011. Selective Isolation of rare Actinomycetes Producing Novel Antimicrobial Compound. International Journal of Advance Biothecnology and Research. 2(3): 357-375. DOI: http://www.bipublication.com
Krieg NR, Holt. 1994. Bergey’s Manual of Determinative Bacteriology. William and Wilkins. London.
Krisnawati H, Sembiring L, Wahyuono, S. 2015. Streptomyces penghasil antibiotik yang berasosiasi dengan rhizosfer beberapa spesies mangrove. Plasma. 1(2): 59-70. DOI: 10.22435/plasma.v1i2.4534.59-70
Li F, Liu S, Lu Q, Zheng H, Osterman IA, Lukyanov DA, Sergiev PV, Dontsova OA, Liu S, Ye J, Huang D, Sun C. 2019. Studies on antibacterial activity and diversity of cultivable actinobacteria isolated from mangrove soil in futian and maoweihai of china. Evidence-Based Complementary and Alternative Medicine. 2019. DOI: https://doi.org/10.1155/2019/3476567
Madigan MT, Martiko JM, Parker J. 2003. Biology of Microorganisms 10th Ed. Pearson Education Inc. USA.
Nurkanto A, Rahmansyah M, Kanti A. 2008. Seri Panduan: Teknik Isolasi Aktinomycetes, Pusat Penelitian Biologi Cibinong Science Center LIPI Bogor
Onu DW, Retnowati Y, Kandowangko N. 2012. Biodiversitas Actinomycetes pada Kawasan Mangrove Desa Bulalo Kecamatan Kwandang dan Uji Potensi Sebagai Penghasil Antibotika. Laporan Penelitian Universitas Negeri Gorontalo.
Prasad M, Kumar S, Kumar U, Anupama. 2015. Screening of endophytic actinomycetes from different indigenous medicinal plants. European Journal of Experimental Biology. 5(4): 7-14.
Pratiwi ST. 2008. Mikrobiologi Farmasi. Erlangga Medical Series. Jakarta.
Purnobasuki H. 2005. Tinjauan Perspektif Hutan Mangrove. AUP. Surabaya.
Ratna KR, Chandrika V. 1999. Effect of different media for isolation growth and maintenance actinomycetes from mangrove sediments. India Journal of Marine Science. 22: 297-299. DOI: http://nopr.niscair.res.in/handle/123456789/37934
Retnowati Y, Sembiring L, Moeljopawiro, Djohan S, Soetarto S, Endang. 2016 Isolasi dan uji aktivitas antibakteri actinomycetes dari rhizosfer bakau di hutan bakau torosiaje gorontalo. Journal of Biological Diversity. 19(6): 293-294. DOI: https://doi.org/10.13057/biodiv/d180259
Sengupta S, Pramanik A, Ghosh A, Bhattacharyya M. 2015. Antimicrobial activities of actinomycetes isolated from unexplored regions of Sundarbans mangrove ecosystem. BMC Microbiology. 15(170): 1-16. DOI: 10.1186/s12866-015-0495-4
Sukma NR. 2017. Kajian pengelolaan mangrove di desa jenu kecamatan jenu kabupaten tuban jawa timur. PENA Akuantika. 15(1): 1-18. DOI: http://dx.doi.org/10.31941/penaakuatika.v15i1.508
Sweetline C, Usha R, Palaniswany M. 2012. Antibacterial activity of actinomycetes from pichavaram mangrove of tamil nadu. Applied Jurnal of Hygiene. 1(2): 15-16. DOI: 10.5829/idosi.ajh.2012.1.2.7183
Wahab A, Shumaila, Subhan AS, Ali TS, Mujahid YT. 2015. Isolation and identification of actinomycetes isolated from karachi soil and screening of antimicrobial compounds. International Jurnal of Current Research. 7: 12761-12762.
Most read articles by the same author(s)
- MUSTOFA HELMI EFFENDI, HAYYUN DURROTUL FARIDAH, FRESHINDY MARISSA WIBISONO, FRESHINTA JELLIA WIBISONO, NABILATUN NISA, FATIMAH, EMMANUEL NNABUIKE UGBO, Detection of virulence factor encoding genes on Escherichia coli isolated from broiler chicken in Blitar District, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 7 (2022)
- FARAH AISYAH NAFIDIASTRI, RIZKY DANANG SUSETYO, TRI NURHARIYATI, AGUS SUPRIYANTO, ALMANDO GERALDI, NI’MATUZAHROH, FATIMAH, SALAMUN, Biosurfactant activity of indigenous Bacillus sp. ES4.3 isolated from endemic breeding sites of dengue hemorrhagic fever vector in Surabaya, East Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 12 (2021)
- APON ZAENAL MUSTOPA, INES FERDIANA PUSPITASARI, FATIMAH FATIMAH, LITA TRIRATNA, GINA KARTINA, Genetic diversity of mastitis cow’s milk bacteria based on RAPD-PCR , Biodiversitas Journal of Biological Diversity: Vol. 19 No. 5 (2018)
- FATIMAH, RAHAYU, JAMAL WIWOHO, SUNNY UMMUL FIRDAUS, PUJIYONO, MARIMIN, DWI PRIYO ARIANTO, AHMAD PRAMONO, Genetic diversity of eucalypts for germplasm conservation in Forest Area with the Special Purpose of Mount Bromo, Karanganyar, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 10 (2021)
- AGMAL QODRI, ENCILIA, YULIZAH, DEDEN GIRMANSYAH, SUNARDI, WAHYUDI SANTOSO, MEGAWATI, RINA RACHMATIYAH, FATIMAH, DARMAWAN, SARINO, DJUNIJANTI PEGGIE, Recorded and predicted butterflies in the Padang Bindu Karst, South Sumatra, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 2 (2023)
- FATIMAH, LILLAH ASRITAFRIHA, SYARIFAH SALSABILA, ERNA KRISTIANA DEWI, NI’MATUZAHROH, ALMANDO GERALDI, RICO RAMADHAN, HERY SUWITO, ARRIZAL RAHMAN, LUKMAN RIYADI, Potential of antimicrobial-producing endophytic bacteria from Balikpapan endemic ginger (Etlingera balikpapanensis A.D. Poulsen) , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 9 (2024)
- ALMANDO GERALDI, CHRISTOPHER CLEMENT, MAYA DEWI PERTIWI, YESYI LESTARI, DHAMMARUCI HEMADHIRA PARINNATA, RIFAID NUR ARSAD, RAMA ALI SADIKIN, ARIF LUQMAN, HERI SANTOSO, ALFINDA NOVI KRISTANTI, YOSEPHINE SRI WULAN MANUHARA, ANJAR TRI WIBOWO, Isolation and characterization of plant growth-promoting bacteria from medicinal plants Java cardamom (Amomum compactum) and bitter ginger (Zingiber zerumbet) , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 6 (2024)
- FATIMAH, NAZIL DWI RAHAYUNINGTYAS, ALIFAH NASTITI, DELA DWI ALAWIYAH, RICO RAMADHAN, ALMANDO GERALDI, JUNAIRIAH, Antibacterial and biosurfactant activity of endophytic bacteria isolated from mangrove plant in Lamongan, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 7 (2024)
- SALAMUN, RIZKY DANANG SUSETYO, NI’MATUZAHROH, FATIMAH, ALMANDO GERALDI, AGUS SUPRIYANTO, TRI NURHARIYATI, FARAH AISYAH NAFIDIASTRI, NABILATUN NISA’, ENDARTO, Biosurfactant production of entomopathogenic Bacillus subtilis BK7.1, as potential biocontrol bacteria, isolated from Baluran National Park, East Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 3 (2023)