Antioxidant activity of various solvent extracts from endophytic bacteria isolated from girang (Leea indica) leaves
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Abstract. Arivo D, Mubarik NR, Rusmana I, Batubara I. 2023. Antioxidant activity of various solvent extracts from endophytic bacteria isolated from girang (Leea indica) leaves. Biodiversitas 24: 415-422. Girang (Leea indica) is one of the medicinal plants with various bioactive compounds that act as an antioxidant. However, isolating these bioactive compounds need a significant amount of biomass. Endophytic bacteria can be a good solution to produce the same bioactive compounds as their host. This study determined whether the potential isolate produces high antioxidant activity in various solvents, phytochemical screening, determination of total flavonoid and phenolic contents, and identification of the isolate. The BT4 isolate was isolated from L. indica leaves. The supernatant of BT4 was extracted in fractionated extraction. The antioxidant activity was determined using a DPPH assay. Folin-Ciocalteu and aluminum chloride colorimetric methods analyzed the total flavonoid and phenolic content. The isolate was identified using the 16S rRNA method. The best IC50 was obtained from n-butanol extract with a value of 59.021±0.541 ?g/mL. The flavonoid and phenolic contents from n-butanol extract have the value of 4.686±0.476 mgQE/g extract and 31.406±0.716 mgGAE/g extract, respectively. The BT4 isolate contained flavonoids, saponin, tannins, terpenoids, and steroids and was identical to Bacillus tequilensis with 99% similarity. This study revealed that endophytic bacteria from L. indica leaves could be a potential source of the novel natural antioxidant compound.
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References
Arivo D, Mubarik NR, Rusmana I, Batubara I. 2021. Isolation, characterization, and screening of antioxidant Activity of endophytic bacteria from Leea Indica (Burm. F) Merr. The 4th International Conference on Biosciences (ICoBio 2021); IOP Conference Series: Earth and Environmental Science 948. Bogor Agricultural University. Bogor, 8-9 August 2022. [Indonesian].
Banjarnahor SDS, Artanti N. 2014. Antioxidant properties of flavonoids. Med J Indones. 23(4): 239-244.
Batubara I, Mitsunaga T, Ohashi H. 2009. Screening antiacne potency of Indonesian medicinal plants?: antibacterial, lipase inhibition, and antioxidant Activities. J wood Sci. 55,230–235. doi:10.1007/s10086-008-1021-1.
Bhattacharya A, Giri VP, Singh SP, Pandey S, Chauhan P, Soni SK, Srivastava S, Singh PC, Mishra A. 2019. Intervention of bio-protective endophyte Bacillus tequilensis. enhance physiological strength of tomato during Fusarium wilt infection. Biol Control. 139: 104074. doi.https://org/10.1016/j.biocontrol.2019.104074.
Bogucka-Kocka A, Zidorn C, Kasprzycka M, Szymczak G, Szewczyk K. 2016. Phenolic acid content, antioxidant and cytotoxic Activities of four Kalanchoë species. Saudi J Biol Sci. 25(4):622–630. doi:10.1016/j.sjbs.2016.01.037.
Dalu D, Duggirala S, Akarapu S. 2014. Anti hyperglicemic and hypolipidemic Activity of Leea Indica. Int J Bioassay. 3:3155–3164. doi:10.13140/RG.2.2.14200.14086.
Devi K, Pandey G, Rawat A, Sharma GD, Pandey P. 2017. The endophytic symbiont-Pseudomonas aeruginosa stimulates the antioxidant Activity and growth of Achyranthes aspera L. Front Microbiol. 8, 1–14. doi:10.3389/fmicb.2017.01897.
Elamin MM. 2020. Solvent extraction and spectroscopy identification of bioactive compounds from medicinal shrub Tamarix gallica. Func. Foods Health Dis.10. 456-464. 10.31989/ffhd.v10i11.744.
Emran TB, Rahman A, Hosen SMZ, Khanam UH, Saha D. 2012. Antioxidant, cytotoxic and phytochemical properties of the ethanol extract of Leea indica leaf. J Pharm Res. 5(5):2938–2941.
Ferrando L and Scavino AF. 2014. Symbiotic endophytes; functional diversity of endophytic bacteria. Vol. 37. Springer: Heidelberg, pp. 1-19.
Ghagane SC, Puranik SI, Kumbar VM, Nerli RB, Jalalpure SS, Hiremath MB, Neelagund S, Aladakatti R. 2017. In vitro antioxidant and anticancer Activity of Leea indica leaf extracts on human prostate cancer cell lines. Integr Med Res. 6, 79–87. doi:10.1016/j.imr.2017.01.004
Harborne JB. 2001. Twenty-five years of chemical ecology. Nat Prod Rep. 18,361–379.
Harun A, Erlena N, Ab A, Azfar M, Jalil A, Daud S, Harith SS, Hassan NM. 2016. Comparative study of antioxidant and antimicrobial Activity of root, stem, and leaves of Leea indica species. Malaysian J Sci. 35,259–274.
Kekuda T, Raghavendra H, Bharadwaj N, Akhilesha S. 2018. Traditional uses, chemistry and pharmacological Activities of Leea indica (Burm. f.) Merr. (Vitaceae): A comprehensive review. Int J Green Pharm. 12, 71–80.
Kumala S, Utji R, Sudarmono P, Kardono LBS. 2007. Cytotoxic Secondary Metabolites from Fermentation Broth of Brucea javanica Endophytic Fungus 1.2.11. Res. J. Microbiol. 2, 625-631.
Kumar J, Singh D, Ghosh P, Kumar A. 2017. Endophytic and Epiphytic Modes of Microbial Interactions and Benefits. In: Kumar J, Singh D, Ghosh P, Kumar A, editor. Endophytic and Epiphytic Modes of Microbial Interactions and Benefits.1st eds Springer Nature Singapore Pte Ltd. 227–253. doi:10.1007/978-981-10-5813-4.
Li H, Guan Y, Dong Y,Zhao L, Rong S, Rong S, Chen W, Miaomiao, Xu H, Gao X, Chen R, Li L, Xu Z. 2018. Isolation and evaluation of endophytic Bacillus tequilensis GYLH001 with potential application for biological control of Magnaporthe oryzae. Plos One. 13, e0203505. doi: 10.1371/journal.pone.0203505.
Maehara S, Ikeda M, Haraguchi H, Kitamura C, Nagoe T, Ohashi K, Shibuya H. 2011. Microbial conversion of curcumin into colorless hydroderivatives by the endophytic fungus Diaporthe sp. associated with Curcuma longa. Chem Pharm Bull (Tokyo). 59, 1042–1044. doi:10.1248/cpb.59.1042
Marchesi JR, Sato T, Weightman AJ, Martin TA. 1998. Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA. Appl Environ Microbiol. 64, 795–799. doi:10.1128/AEM.64.6.2333-2333.1998.
Mishra G, Khosa RI, Singh P, Jha KK. 2014. Hepatoprotective Activity of ethanolic extract of Leea indica (Burm.F.) Merr. (Leeaceae) stem bark against paracetamol induced liver toxicity in rat. Niger J Exp Clin Biosci. 2(1):59-63.
Minsas S, Nurdiansyah SI, Prayitno DI, Juane Sofiana MS, Kalija TA, Fadly D, Warsidah. 2020. Screening of bioactive compounds and antioxidant Activity of ale-ale shellfish (Meretrix meretrix) crude extracts from west kalimantan, Indonesia. Syst Rev Pharm. 11, :222–227.
Molyneux P. 2004. The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant Activity. Songklanakarin J Sci Technol. 26, 211–219. doi:10.1287/isre.6.2.144.
Nisa S, Shoukat M, Bibi Y, Al-Ayoubi S. 2022. Therapeutic Prospects of Endophytic Bacillus species from Berberis lycium against Oxidative Stress and Microbial Pathogens. Saudi J. Biol Sci. 29, 1-9. doi: 10.1016/j.sjbs.2021.08.099.
Orhan N, Orhan I, Ergun F. 2013. Insights into cholinesterase Inhibitory and intioxidant Activities of Five Juniperus Species. Food Chem Toxicol. 49,2305–2312.
Prastya ME, Astuti RI, Batubara I, Wahyudi AT. 2018. Bacillus sp. SAB E-41-derived extract shows antiaging properties via ctt1-mediated oxidative stress tolerance response in yeast Schizosaccharomyces pombe. Asian Pac J Trop Biomed. 8(11):533–539. doi:10.4103/2221-1691.245958.
Pudjas NTG, Mubarik NR, Astuti RI, Sudirman LI. 2022. Antioxidant Activity of endophytic bacteria derived from Hoya multiflora Blume Plant and Their Cellular Activities on Schizosaccharomyces pombe. HAYATI J. Biosci. 29, 214-221. doi: https://doi.org/10.4308/hjb.29.2.214-221
Quettier-Deleu, C. 2000. Phenolic compounds and antioxidant Activities of uckwheat (Fagopyrum esculentum Moench) Hulls and Flour. J of Ethnopharm, 72, 35-42. doi: https://doi.org/10.1016/S0378-8741(00)00196-3.
Rahman MA, Imran T bin, Islam S. 2012. Antioxidative, antimicrobial and cytotoxic effects of the phenolics of Leea indica leaf extract. Saudi J Biol Sci. 20(3):213–225. doi:10.1016/j.sjbs.2012.11.007.
Raihan M, Habib M, Brishti A, Rahman M, Saleheen M, Manna M. 2011. Sedative and anxiolytic effects of the methanolic extract of Leea indica (Burm. f.) Merr. leaf. Drug Discov Ther. 5(4):185–189. doi:10.5582/ddt.2011.v5.4.185.
Ramesh D, Ramesh D, Kekuda PTR, Onkarappa R, Vinayaka KS, Raghavendra HL. 2015. Antifungal and radical scavenging Activity of leaf and bark of Leea indica (Burm. f.) Merr. J Chem Pharm Res. 7(1):105–110.
Ramkissoon JS, Mahomoodally MF, Ahmed N, Subratty AH. 2013. Antioxidant and anti-glycation Activities correlates with phenolic composition of tropical medicinal herbs. Asian Pac J Trop Med. 6 (7): 561-569. doi: https://doi.org/10.1016/S1995-7645(13)60097-8.
Reetz MT, König G. 2021. n-Butanol: An Ecologically and economically viable extraction solvent for isolating polar products from aqueous solutions. EurJOG. 46, 6224–6228.
Reddy NS, Navanesan S, Sinniah SK, Wahab NA, Sim KS. 2012. Phenolic content, antioxidant effect and cytotoxic Activity of Leea indica leaves. BMC Complement Altern Med. 12(128) :1–7. doi: http://www.biomedcentral.com/1472-6882/12/128.
Ridsdale C. 1974. A Revision of The Family Leeaceae. Blumea biodiversity, Evol Biogeogr Plants. 22,57–100.
Road C dan Saraf M. 2020. isolation and characterization of t-resveratrol and ?-viniferin, a bioactive secondary metabolite on an endophytic fungus Aspergillus stellifer AB4, from Vitis vinifera. JMBFS. 9(4) :708-7013. doi: 10.15414/jmbfs.2020.9.4.708-713.
Saha K. 2005. The synthesis and bioactivity study of anthraquinine and the isolation of bioactive compounds from Leea indica (Burm.F.) Merr. [Thesis]. Malaysia: Universiti Putra Malaysia.
Sasidharan S, Chen Y, Saravanan D, Sundram KM, Latha LY, Bedong-semeling J, Nasi BA. 2011. Extraction , isolation and cahracterization of bioactive compounds from plants extract. African J Tradit Complement Altern Med. 8,1–10.
Shabira AP, Tjahjoleksono A, Lestari Y. 2022. Endophytic actinobacteria of Eleutherine palmifolia as antioxidant producer. Biodiversitas. 23(8): 4209-4215. DOI: 10.13057/biodiv/d230844.
Singh M, Kumar A, Singh R, Pandey KD. 2017. Endophytic bacteria: a new source of bioactive compounds. 3 Biotech. 7(5):1-14. doi:10.1007/s13205-017-0942-z.
Singh D, Siew Y, Chong T, Yew H, Ho SS, Lim C sophie E-S, Tan W-S, Neo S-Y, Koh H. 2019. Identification of phytoconstituents in Leea indica. Molecules. 24,714–724.
Stonik VA. 1986. Some terpenoid and steroid derivatives from echinoderms and sponges. Pure Appl Chem. 58,423–436.
Sulmartiwi L, Pujiastuti DY, Tjahjaningsih W, Jariyah. 2017. Potential of mangrove Avicennia rumphiana extract as an antioxidant agent using multilevel extraction. In: proceedings of the ASEAN Fen International Fisheries Symposium, Surabayas, 137, 1-6.
Tiwari, P., Kumar, B., Kaur, M., Kaur, G., & Kaur, H. 2011. Phytochemical screening and extraction: a review. IPS, 1, 98-106.
Sulistyaningsih E, Amalia TY, Kartikasari R. 2017. Antioxidant and antimalarial Activity of Leea indica leaf extract against malaria-mice model. J Appl Pharm Sci. 7,163–168. doi:10.7324/JAPS.2017.71223.
Wu W, Chen W, Liu S, Wu J, Zhu Y, Qin L, Zhu B. 2021. Beneficial relationships between endophytic bacteria and medicinal plants environmental effects on community. Front Plant Sci. 12,1–13. doi: 10.3389/fpls.2021.646146.
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