Abstract. Habisukan UH, Elfita, Widjajanti H, Setiawan A, Kurniawati AR. 2021. Diversity of endophytic fungi in Syzygium aqueum. Biodiversitas 22: 1129-1137. Exploring endophytic fungi may provide alternative, plant-based ethnomedicines. The discovery of endophytic fungi can produce many plant-derived drugs that give new horizons to the pharmaceutical industry for the availability and production of such medicines on a large scale. Extracts from Syzygium aqueum Alston (jambu air), a fruit-bearing plant native to Indonesia, have long been used in traditional medicine. Therefore, the purpose of this study was to identify, and investigate, the antimicrobial activity of the endophytic fungi found in S. aqueum. Results revealed that total16 fungi were isolated . Fungal endophytes were identified through morphological characterization and subsequently compared with key fungal identification books. The ethyl acetate extracts of the isolated endophytic fungi were screened for their antimicrobial activity through paper disc diffusion assay fungi isolated from S. aqueum were identified as: Aspergillus niger (Isolate code R21, R41, T72), Cylindrocarpon sp. (Isolate code R11, R31), Trichoderma aureoviridae (Isolate code T11), Trichoderma harzianum (Isolate code T21), Trichoderma sp. (Isolate code T31), Pestalotia sp. (Isolate code T41and T62), Beltrania sp. (Isolate code T52), Chaetomium sp. (Isolate code L11), Cochliobolus sp. (Isolate code L22), Penicillium sp. (Isolate code L32), Cylindrocladium sp. (Isolate code L42), and an unidentified Ascomycota (T51). The values of Shannon-Weiner and Simpson diversity indexes for the overall fungal community were H?=2.133 and 1-D=0.859 respectively. All isolates showed potential antimicrobial activity against Escherichia coli, Salmonella Typhi, and Staphylococcus aureus, but the lowest activity against Candida albicans. The results indicate that the bioactive compounds and secondary metabolites of these isolates should be further investigated for pharmaceutical applications.
Cock I E and Cheesman M 2018 Plants of the genus Syzygium (Myrtaceae): A review on ethnobotany, medicinal properties and phytochemistry. Bioactive Compounds of Medicinal Plants: Properties and Potential for Human Health; Goyal, MR, Ayeleso, AO, Eds, 35-84.
Daglia M. 2012. Polyphenols as antimicrobial agents. Current Opinion In Biotechnology. 23(2), 174-181.
Elfita E, Mardiyanto M, Fitrya F, Larasati J, Julinar J, Widjajanti H, and Muharni M. 2019. Antibacterial activity of Cordyline fruticosa leaf extracts and its endophytic fungi extracts. Biodiversitas 20(12): 3804-3812.
Elfita E, Munawar M, Muharni M, and Suprayetno S. 2013. New pyran of an endophytic fungus Fusarium sp. isolated from the leaves of brotowali (Tinaspora crispa). Indo J Chem 13 (3): 209 – 215.
Gustianingtyas M, Herlinda S, Suwandi, Suparman, Hamidson H, Hasbi, Setiawan A, Verawaty M, Elfita, Arsi. 2020. Toxicity of entomopathogenic fungal culture filtrate of lowland and highland soil of South Sumatra (Indonesia) against Spodoptera litura larvae. Biodiversitas 21: 1839-1849.
Hammer Ø, Harper DAT, Ryan PD. 2001. PAST:Paleontological statistics software package for education and data analysis. Paleontology Electron 4:4–9
Hariyati T, Soelistya D, Jekti D S D and Andayani Y. 2015. Pengaruh ekstrak etanol daun jambu air (Syzygium aqueum) terhadap bakteri isolat klinis. Journal Penelitian Pendidikan IPA. 1(2), 31–38.
Jumpathong J, Abdalla, M A, Lumyong S and Laatsch H. 2010. Stempholgalactoside, a new stemphol derivative isolated from the tropical endophytic fungus Gaeumannomy cesamomi. Natural Product Communications. 5(4), 1934578X1000500415.
Kumar DSS, Hyde KD .2004. Biodiversity and tissue-recurrence of endophytic fungi in Tripterygium wilfordii. Fungal Diversity 17, 69– 90.
Marinova D, Ribarova F and Atanassova M. 2005. Total phenolics and total flavonoids in Bulgarian fruits and vegetables. Journal of The University of Chemical Technology and Metallurgy. 40(3), 255-260.
Muharni M, Fitrya F, Ruliza MO, Susanti DA, Elfita E. 2014. Di-(2-ethylhexyl)phthalate and pyranone derivated from endophytic fungi Penicillium sp. the leave of Kunyit Putih (Curcuma zedoaria). Indon J Chem 14(3): 290-296.
Nicoletti, R., & Fiorentino, A. 2015. Plant bioactive metabolites and drugs produced by endophytic fungi of Spermatophyta. Agriculture, 5(4), 918-970.
Osman H, Rahim A A, Isa N M and Bakhir N M. 2009. Antioxidant activity and phenolic content of Paederiafoetida and Syzygium aqueum. Molecules. 14(3), 970-978.
Palanisamy U D, Ling L T, Manaharan T and Appleton D. 2011. Rapid isolation of geraniin from Nephelium lappaceum rind waste and its anti-hyperglycemic activity. Food Chemistry. 127, 21–27.
Palanisamy U D, Ling L T, Manaharan T, Sivapalan V, Subramaniam T, Helme M H and Masilamani T. 2011. Standardized extract of Syzygium aqueum: a safe cosmetic ingredient. International Journal of Cosmetic Science. 33(3), 269-275.
Panggabean G. 1992. Syzygium aqueum, Syzygium malaccense and Syzygium samarangense: Edible Fruits and Nuts, 2nd ed. Prosea Foundation Bogor, Indonesia.
Photita W, Lumyong S, Lumyong P, Hyde KD. 2001. Endophytic fungi of wild banana (Musa acuminata) at Doi Suthep Pui National Park, Thailand. Mycology Resource, 105,1508–1513.
Pitt J I and A D Hocking. 2009. Fungi and Food Spoilage. Springer Science + Business Media, New York.
Rivera-Orduña, F. N., Suarez-Sanchez, R. A., Flores-Bustamante, Z. R., Gracida-Rodriguez, J. N., & Flores-Cotera, L. B. (2011). Diversity of endophytic fungi of Taxus globosa (Mexican yew). Fungal Diversity, 47(1), 65-74.
Rodrigues KF.1994. The foliar fungal endophytes of the Amazonian palm Euterpe oleracea. Mycologia, 86:376–385
Schulz, B. Boyle, C. Draeger, S. Römmert, A.K. Krohn, K. 2002. Endophytic fungi: A source of novel biologically active secondary metabolites. Mycolology Resources 106, 996–1004.
Shannon CE and Weaver W. 1949. The mathematical theory of communication. University of Illinois Press, Urbana.
Simpson EH. 1949. Measurement of species diversity. Nature (Lond) 163:688.
Sridhar KR, Raviraja NS. 1995. Endophytes—a crucial issue. Current science 69:570–571.
Strobel, G., & Daisy, B. 2003. Bioprospecting for microbial endophytes and their natural products. Microbiology and molecular biology reviews, 67(4), 491-502.
Tehrani M, Sharif Hossain A B M and Nasrulhaq-Boyce A. 2011. Post harvest physico-chemical and mechanical changes in'Jambu Air (Syzygium aqueum Alston) fruits. Australian Journal of Crop Science. 5(1), 32.
Wang Y, Guo LD, Hyde KD.2005. Taxonomic placement of sterile morphotypes of endophytic fungi from Pinus tabulaeformis (Pinaceae) in northeast China based on rDNA sequences. Fungal Divers 20:235–260.
Watanabe T. 2002. Pictorial Atlas of Soil and Seed Fungi : Morphologies of Cultured Fungi and Key to Species 2nd ed. CRC Press LLC, Florida.
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