Biodiversity and antibacterial activity of endophytic fungi isolated from jambu bol (Syzygium malaccense)




Abstract. Hapida Y, Elfita, Widjajanti H, Salni. 2021. Biodiversity and antibacterial activity of endophytic fungi isolated from jambu bol (Syzygium malaccense). Biodiversitas 22: 5668-5677. Infectious diseases caused by pathogenic bacteria are still high. The existence of bacterial resistance to antibiotics makes it necessary to search for new antibacterial substances from plants and microorganisms. Syzygium malaccense (L.) Merr and Perry (Myrtaceae), known as jambu bol, has been used as a traditional medicine in Indonesia and other countries. It is used to treat pathogenic bacterial infections. Therefore, this study aims to determine the diversity of endophytic fungi and their potential as antibacterial compound sources compared to their host plants. Specifically, screening for endophytic fungi that have antibacterial activities is intended. In this study, endophytic fungi were isolated from the leaves, stem bark, and root bark of the host plants. Each isolate was identified morphologically and cultivated in potato dextrose broth (PDB) medium for four weeks. After the incubation period, the liquid culture was extracted with ethyl acetate and evaporated to obtain a concentrated extract. The Kirby-Bauer method was used to evaluate the antibacterial activity of each endophytic fungal extract and the host plant parts extract. The concentration used was 400µg/mL and the antibiotic was used as a positive control (Tetracycline) is 30µg/mL. Seven endophytic fungi were isolated from the leaves (YD1, YD2, YD3, YD4, YD6, YD7, Y2D1), seven from the stem bark (YB1, YB2, YB3, YB4, YB5, Y2B5, and Y2B6 ), and six from the root bark (YA1, YA2, YA3, YA4, YA5, Y2A2). The endophytic fungi were morphologically identified, and the isolates were shown to be dominated by genera from the Ascomycota (e.g., Poaceascoma, Cladorrhinum, Penicillium, Madurella, Phytophthora, Phialemonium, Monascus, Trichoderma, Ramophialophora, Gliocladium, Wiesneriomyces) and the Zygomycota (e.g., Mucor, Mortierella, and Gongronella) phyla. The fungi isolates Y2D1, YB1, and YA4 were shown to have the highest (strong) antibacterial activity equivalent to their hosts in the performed screening. Endophytic fungi have significant and continued antibacterial activities that have been identified molecularly.


Abdalla, M. A., & McGaw, L. J. (2018). Bioprospecting of South African plants as a unique resource for bioactive endophytic microbes. Frontiers in Pharmacology, 9(MAY).
Ahamed, A., & Ahring, B. K. (2011). Bioresource Technology Production of hydrocarbon compounds by endophytic fungi Gliocladium species grown on cellulose. Bioresource Technology, 102(20), 9718–9722.
Arzamani, K., Vatandoost, H., Rassi, Y., & Akhavan, A. A. (2018). Original Article Richness and Diversity of Phlebotomine Sand Flies ( Diptera?: Psychodidae ) in North Khorasan Province , Northeast of Iran. 12(September), 232–239.
Bairy, K., Sharma, A., Shalini, & Adiga. (2005). Evaluation of the hypoglycemic, hypolipidemic and hepatic glycogen raising effects of Syzygium malaccense upon streptozotocin induced diabetic rats. Journal of Natural Remedies, 5(1), 46–51.
Batista, Â. G., da Silva, J. K., Betim Cazarin, C. B., Biasoto, A. C. T., Sawaya, A. C. H. F., Prado, M. A., & Maróstica Júnior, M. R. (2017). Red-jambo (Syzygium malaccense): Bioactive compounds in fruits and leaves. LWT - Food Science and Technology, 76, 284–291.
Bi, M. A., & Gajalakshmi, P. (2018). Antibacterial and antioxidant activity of the pigment produced by monascus purpureus. Eurasian Journal of Analytical Chemistry, 13(2), 378–384.
Chatterjee, S., Ghosh, R., Chandra, N., & Id, M. (2019). Production of bioactive compounds with bactericidal and antioxidant potential by endophytic fungus Alternaria alternata AE1 isolated from Azadirachta indica A . Juss . 1–18.
Chauhan, N. M., Gutama, A. D., & Aysa, A. (2019). Endophytic fungal diversity isolated from different agro-ecosystem of Enset (Ensete ventericosum) in Gedeo zone, SNNPRS, Ethiopia. BMC Microbiology, 19(1), 1–10.
Chutulo, E. C., & Chalannavar, R. K. (2018). Endophytic mycoflora and their bioactive compounds from azadirachta indica: A comprehensive review. Journal of Fungi, 4(2).
Cruz, T. E. E. dela, Notarte, K. I. R., Carlo Chris S. Apurillo, K. T., Bungihan, & E., M. (2020). Biomining fungal endophytes from tropical plants and seaweeds for drug discovery. Biodiversity and Biomedicine Their, (October).
Cruz, J., da Silva, C., & Hamerski, L. (2020). Natural products from endophytic fungi associated with rubiaceae species. Journal of Fungi, 6(3), 1–26.
da Silva Dantas, C. C., de Souza, E. L., Cardoso, J. D., de Lima, L. A., de Sousa Oliveira, K., Migliolo, L., … Magnani, M. (2014). Identification of a Napin-Like Peptide from Eugenia malaccensis L. Seeds with Inhibitory Activity Toward Staphylococcus aureus and Salmonella Enteritidis. Protein Journal, 33(6), 549–556.
Ding, N., Chang, X., Shi, N., Yin, X., Qi, F., & Sun, Y. (2019). Enhanced inactivation of antibiotic-resistant bacteria isolated from secondary effluents by g-C 3 N 4 photocatalysis. 18730–18738.
El-Bondkly, E. A. M., El-Bondkly, A. A. M., & El-Bondkly, A. A. M. (2021). Marine endophytic fungal metabolites: A whole new world of pharmaceutical therapy exploration. Heliyon, 7(3), e06362.
El-Hawary, S. S., Moawad, A. S., Bahr, H. S., Abdelmohsen, U. R., & Mohammed, R. (2020). Natural product diversity from the endophytic fungi of the genusAspergillus. RSC Advances, 10(37), 22058–22079.
Fadhillah, F., Elfita, E., Muharni, M., & Yohandini, H. (2021). Antioxidant activity of endophytic fungi isolated from the stem bark of Swietenia mahagoni (L.) Jacq. IOP Conference Series: Materials Science and Engineering, 1011(1).
Fadiji, A. E., & Babalola, O. O. (2020). Exploring the potentialities of beneficial endophytes for improved plant growth. Saudi Journal of Biological Sciences, 27(12), 3622–3633.
Ferde?, M., Ungureanu, C., Radu, N., Chirvase, A. A., & Independentei, S. (2004). Antimicrobial effect of Monascus purpureus red rice against some bacterial and fungal strains. 1–6.
Fernandes, F. A. N., & Rodrigues, S. (2018). Jambo — Syzygium malaccense. In Exotic Fruits Reference Guide.
Fitriarni, D., & Kasiamdari, R. S. (2018). Isolation and Identification of Endophytic Fungi from Leave and Stem of Calopogonium mucunoides. Journal of Tropical Biodiversity and Biotechnology, 3(1), 30.
Freitas, T., Pereira, L., & Pereira, C. (2015). Syzygium sp (Myrtaceae): Promising for Diabetes Treating? European Journal of Medicinal Plants, 7(4), 167–176.
Gouda, S., Das, G., Sen, S. K., & Shin, H. (2016). Endophytes?: A Treasure House of Bioactive Compounds of Medicinal Importance. 7(September), 1–8.
Habisukan, U. H., Elfita, E., Widjajanti, H., & Setiawan, A. (2021). Diversity of endophytic fungi in Syzygium aqueum. 22(3), 1129–1137.
Jia, M., Chen, L., Xin, H. L., Zheng, C. J., Rahman, K., Han, T., & Qin, L. P. (2016). A friendly relationship between endophytic fungi and medicinal plants: A systematic review. Frontiers in Microbiology, 7(JUN), 1–14.
John I. Pitt, & Hocking, A. D. (2009). Fungi and food Spoilage. In Angewandte Chemie International Edition, 6(11), 951–952. (Preface to). 10.1007/978-0-387-92207-2_1
Locher, C. P., Burch, M. T., Mower, H. F., Berestecky, J., Davis, H., Van Poel, B., … Vlietinck, A. J. (1995). Anti-microbial activity and anti-complement activity of extracts obtained from selected Hawaiian medicinal plants. Journal of Ethnopharmacology, 49(1), 23–32.
Manganyi, M. C., & Ateba, C. N. (2020). Untapped potentials of endophytic fungi: A review of novel bioactive compounds with biological applications. Microorganisms, 8(12), 1–25.
Oyinlade, O. C. (2014). Phytochemical and Physicochemical Analysis of Three Different Types of Apples. International Journal of Scientific Research and Reviews Phytochemical and Physicochemical Analysis of Three Different Types of Apples, 3(1), 67–78. Retrieved from
Ozimek, E., & Hanaka, A. (2021). Mortierella species as the plant growth-promoting fungi present in the agricultural soils. Agriculture (Switzerland), 11(1), 1–18.
Pelo, S., & Mavumengwana, V. (2020). Diversity and Antimicrobial Activity of Culturable Fungal Endophytes in Solanum mauritianum.
Ruangwong, O., Wonglom, P., Suwannarach, N., Kumla, J., Thaochan, N., Chomnunti, P., … Sunpapao, A. (2021). Volatile Organic Compound from Trichoderma asperelloides TSU1?: Impact on Plant Pathogenic Fungi. 1–13.
Rufin Marie Kouipou Toghueo and, & Boyom, F. F. (2020). Endophytic Penicillium species and their agricultural , biotechnological , and pharmaceutical applications. In 3 Biotech (Vol. 10).
Segaran, G., & Sathiavelu, M. (2019). Fungal endophytes: A potent biocontrol agent and a bioactive metabolites reservoir. Biocatalysis and Agricultural Biotechnology, 21(May), 101284.
Selim, K. A., El-Beih, A. A., AbdEl-Rahman, T. M., & El-Diwany, A. I. (2012). Biology of Endophytic Fungi. Current Research in Environmental & Applied Mycology, 2(1), 31–82.
Singh, A., Singh, D. K., Kharwar, R. N., White, J. F., & Gond, S. K. (2021). Fungal Endophytes as Efficient Sources of Plant-Derived Bioactive Compounds and Their Prospective Applications in Natural Product Drug Discovery?: Insights , Avenues , and Challenges.
Sonavane, P. (2017). First Report of Collectotrichum acutatum J.H. Simmonds Associated with Anthracnose Disease in Malayan Apple from India. International Journal of Pure & Applied Bioscience, 5(5), 465–472.
Stierle, A. A., & Stierle, D. B. (2016). Bioactive Secondary Metabolite by the Fungal Endophytes of Conifers. 10(10), 1671–1682.
Strobel, G., & Daisy, B. (2003). Bioprospecting for Microbial Endophytes and Their Natural Products. Microbiology and Molecular Biology Reviews.
Verma, V. C., Kharwar, R. N., & Strobel, G. A. (2009). Chemical and functional diversity of natural products from plant associated endophytes. (May 2014).
Walsh, T. J., Hayden, R. T., & Larone, D. H. (2018). Larone’s Medically Important Fungi. In Larone’s Medically Important Fungi.
Watanabe, T. (2010). Identification of Fungi. Pictorial Atlas of Soil and Seed Fungi, 9–11.
Zhao, J., Zhou, L., Wang, J., Shan, T., Zhong, L., Liu, X., & Gao, X. (2010). Endophytic fungi for producing bioactive compounds originally from their host plants. (January), 567–576.

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