Characteristics of anti-Vibrio harveyi compounds produced by Bacillus spp. isolated from shrimp ponds




Abstract. Rusmana I, Isramilda, Akhdiya A. 2021. Characteristics of anti-Vibrio harveyi compounds produced by Bacillus spp. isolated from shrimp ponds. Biodiversitas 22: 4872-4879. Pathogenic Vibrio spp. such as Vibrio vulnificus, V. alginolyticus, V. fluvialis, V. anguillarum, and V. harveyi caused shrimp diseases. The application of bacterial probiotics can control the growth of pathogenic Vibrio in shrimp. Bacillus spp. can produce antimicrobial compounds that inhibit the growth of pathogenic Vibrio spp. Isolation of Bacillus from several pond water samples, pond sediment, and shrimp intestines was successfully collected 175 isolates of Bacillus spp. Three isolates, i.e., Ltw54, Lts36, dan Lts40 had a high inhibitory index and stability in inhibiting the growth of Vibrio harveyi. Verification assay showed that Bacillus sp. Lts40 had the highest antimicrobial activity. The competition assay showed that Bacillus sp Lts40 isolates inhibited the growth of V. harveyi up to 81,8%. The antimicrobial compound produced by Bacillus sp. Lts40 was stable at the pH range of 3-11 and remained stable after heating at 100ºC for 20 minutes. The purification results using the chromatographic filtration technique showed that the protein fraction with a molecular weight of 47,38 kDa effectively inhibited V. harveyi growth. Bacillus sp. Lts40 isolate has potential application as a probiotic agent in shrimp ponds to control the growth of V. harveyi that causes luminous vibriosis diseases and the antimicrobial substance is potentially to be developed and produced as an anti-V. harveyi product that can be applied in a shrimp hatchery.


Ahn C and Stiles ME 1990 Plasmid associated bacteriocin production by a strain Carnobacterium piscicola from meat Appl Environ Microbiol 56 2503. DOI:10.1128/aem.56.8.2503-2510.1990
Anastasios A and George K 2003 Purification and characterization of thermophilic ST-1, a novel bacteriocin produced by Streptococcus thermophilus ACA-DC 0001. J Food Sci Technol 83 365. DOI:10.1051/lait:2003024
Benoit V, Mathis R and Lefebure G 1994 Characterization of Breviscin 27, a bacteriocin synthesized by Lactobacillus Brevis SB27. Curr Microbiol 28 53. DOI:10.1007/BF01575986
Bhunia AK, MC Johnson, and Ray B 1988 Purification, characterization and antimicrobial spectrum of a bacteriocin produced by Pediococcus acidilactici J Appl Bacteriol 65 261. DOI:10.1111/j.1365-2672.1988.tb01893.x
Bradford MM 1976 A rapid and sensitive method for quantitating microgram quantities of protein utilizing principles of protein-dye binding. Anal Biochem 72 248. DOI:10.1016/0003-2697(76)90527-3
Chythanya R, Indrani K and Iddya K 2002 Inhibition of shrimp pathogenic vibrios by a marine Pseudomonas 1-2 strain. Aquaculture 208 1. DOI:10.1016/S0044-8486(01)00714-1
Cintas LM et al. 1995 Isolation and characterization of Pediocin L50 a new bacteriocin from Pediococcus acidilactici with a broad inhibitory spectrum Appl Environ Microbiol 61 2643. DOI:10.1128/aem.61.7.2643-2648.1995
Dajani AS and Wannamaker LW 1969 Demonstrated a bactericidal substance against beta-hemolytic streptococci in supernatant fluid of staphylococcal cultures. J Bacteriol 97 985. DOI:10.1128/jb.97.3.985-991.1969
De Souza Valente C and Wa A H L 2021 Vibrio and major commercially important vibriosis diseases in decapod crustaceans. Journal of Invertebrate Pathology 181 107527. DOI:10.1016/j.jip.2020.107527
Denniston et al. 2001 General, Organic and Chemistry Eds-3 (New York: Mc Graw Hill)
Frazier WC and Westhoff DC 1981 Food Microbiology (New Delhi: Tata Mc Graw-Hill)
Harper E, Santoso L, Supono S, Wardiyanto W, Widodo A and Yolanda L 2018 Effects of dietary probiotic Bacillus sp. D2. 2 and prebiotic sweet potato extract on growth performance and resistance to Vibrio harveyi in Pacific white shrimp, Litopenaeus vannamei Aquaculture Indonesiana 18 55. DOI:10.21534/ai.v18i2.107
Hieronymus B, Marrec CL, and Urdaci MC 1998 Coagulin, a bacteriocin-like inhibitory substance produced by Bacillus coagulans I4 J Appl Microbiol 85 42. DOI:10.1046/j.1365-2672.1998.00466.x
Irina VP, Philippe B, Bernard V and Bernard F 2001 In vitro anti-Helicobacter pylori activity of the probiotic strain B. subtilis 3 is due to secretion of antibiotic J Antimicrob Agent Chemother 45 3156. DOI:10.1128/AAC.45.11.3156-3161.2001
Jo YB, Kyung MB, Song-Koo K and Hong-ki J 1996 Evaluation at optimum conditions for bacteriocin production from Lactobacillus sp. JB-42 isolated from kimchi J Microbiol Biotechnol 6 63
Joshi VK, Sharma S and Rana NS 2006 Production, purification, stability and efficacy of bacteriocin from isolates of natural lactic acid fermentation of vegetables. J Food Technol Biotechnol 44 435
Kato F et al. 1991 Purification and characterization of Linecin A, a bacteriocin of Brevibacterium linens. J Agric Biol Chem 55 161. DOI:10.1271/bbb1961.55.161
Kone K and Fung YC. 1992. Understanding bacteriocins and their uses in food J Food Environ Sanit 12 282
Kumar T S, Vidya R, Kumar S, Alavandi SV, and Vijayan KK 2017 Zoea-2 syndrome of Penaeus vannamei in shrimp hatcheries Aquaculture 2017 07. 022 DOI: 10.1016/j.aquaculture.2017.07.022
Lee KH, Jun KD, Kim WS and Paik HO 2001 Partial characterization of polyfermenticin SCD, a newly identified bacteriocin of Bacillus polyfermenticus Appl Microbiol 32 146. DOI:10.1046/j.1472-765x.2001.00876.x
Lisboa M P et al. 2006 Characterization of a bacteriocin-like substance produced by Bacillus amyloliquefaciens isolated from the Brazilian Atlantic Forest J Inten Microbiol 9 111
Martirani LM, Varcamonti, Naclerio G and Felice MD 2002 Purification and partial characterization of bacillon 490 a novel bacteriocin produced by a thermophilic strain of Bacillus licheniformis. J Microbiol Cell Factor 1 1. DOI:10.1186/1475-2859-1-1
Mokoena M P 2017. Lactic acid bacteria and their bacteriocins: classification, biosynthesis, and applications against uropathogens: a mini-review. Molecules 22 1255. DOI:10.3390/molecules22081255
Ogunbanwo ST, Sanni AI and Onilude AA 2003 Characterization of bacteriocin produced by Lactobacillus Plantarum F1 and Lactobacillus Brevis OG1 J Biotechnol 2 219. DOI:10.5897/AJB2003.000-1045
Oh S, Kim SH and Worobo RW 2000 Characterization and purification of a bacteriocin produced by a potential probiotic culture Lactobacillus acidophilus 30SC. J Sci Technol 83 2747. DOI:10.3168/jds.S0022-0302(00)75169-1
Oscariz JC and Pisabarro AG 2000 Characterization and mechanism of action of cerein 7, a bacteriocin produced by Bacillus cereus Bc 7 J Appl Microbiol 89 361. DOI:10.1046/j.1365-2672.2000.01123.x
Parada J L, Caron C R, Medeiros A BP and Soccol C R 2007 Bacteriocins from lactic acid bacteria: purification, properties and use as preservatives. Braz. Arch. Biol. Technol. 50 521. DOI:10.1590/S1516-89132007000300018
Rachmaniar R 1997 Potensi spon asal kepulauan spermonde sebagai antimikroba. Seminar perikanan Indonesia II. Ujung Pandang 2-3 Desember 1997.
Ray B and Daeschel M 1992 Food Biopreservative of Microbiology Origin (London: CRC Press)
Roza D Z 1993 Teknik penanggulangan penyakit udang menyala di hatchery melalui pengendalian populasi bakteri J Penel Budidaya Pantai 9 127
Torkar KG and Matijasic BB 2003 Partial characterization of bacteriocins produced by Bacillus cereus isolates from milk and milk products Food Technol Biotechnol 41 121
Verschuere L, Rombaut G, Sorgeloos P and Verstraete W 2000 Probiotic bacteria as control agent in aquaculture J Mol Biol Biotechnol 64 655. DOI:10.1128/MMBR.64.4.655-671.2000

Most read articles by the same author(s)

1 2 > >>